{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:33:06Z","timestamp":1790735586359,"version":"4.1.0"},"reference-count":24,"publisher":"Society for Neuroscience","issue":"39","license":[{"start":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T00:00:00Z","timestamp":1395705600000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2013,9,25]]},"abstract":"<jats:p>Niemann-Pick type C1 (NPC1) disease is a rare neurovisceral disorder characterized by intracellular accumulation of unesterified cholesterol, sphingolipids, and other lipids in the lysosomal compartment. A deregulation of lysosomal calcium has been identified as one of the earliest steps of the degenerative process. Since adenosine A<jats:sub>2A<\/jats:sub>receptors (A<jats:sub>2A<\/jats:sub>Rs) control lysosome trafficking and pH, which closely regulates lysosomal calcium, we hypothesized a role for these receptors in NPC1. The aim of this study was to evaluate the effects of the A<jats:sub>2A<\/jats:sub>R agonist CGS21680 on human control and NPC1 fibroblasts. We show that CGS21680 raises lysosomal calcium levels and rescues mitochondrial functionality (mitochondrial inner membrane potential and expression of the complex IV of the mitochondrial respiratory chain), which is compromised in NPC1 cells. These effects are prevented by the selective blockade of A<jats:sub>2A<\/jats:sub>Rs by the antagonist ZM241385. The effects of A<jats:sub>2A<\/jats:sub>R activation on lysosomal calcium are not mediated by the cAMP\/PKA pathway but they appear to involve the phosphorylation of ERK1\/2. Finally, CGS21680 reduces cholesterol accumulation (Filipin III staining), which is the main criterion currently used for identification of a compound or pathway that would be beneficial for NPC disease, and such an effect is prevented by the Ca<jats:sup>2+<\/jats:sup>chelator BAPTA-AM. Our findings strongly support the hypothesis that A<jats:sub>2A<\/jats:sub>R agonists may represent a therapeutic option for NPC1 and provide insights on their mechanisms of action.<\/jats:p>","DOI":"10.1523\/jneurosci.0558-13.2013","type":"journal-article","created":{"date-parts":[[2013,9,25]],"date-time":"2013-09-25T16:47:08Z","timestamp":1380127628000},"page":"15388-15393","source":"Crossref","is-referenced-by-count":37,"title":["The Stimulation of Adenosine A<sub>2A<\/sub>Receptors Ameliorates the Pathological Phenotype of Fibroblasts from Niemann-Pick Type C Patients"],"prefix":"10.1523","volume":"33","author":[{"given":"Sergio","family":"Visentin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"De Nuccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonietta","family":"Bernardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Pepponi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Ferrante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Minghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Popoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2013,9,25]]},"reference":[{"key":"2023041304070583000_33.39.15388.1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20896"},{"key":"2023041304070583000_33.39.15388.2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000333215.93440.36"},{"key":"2023041304070583000_33.39.15388.3","doi-asserted-by":"publisher","DOI":"10.3791\/4228"},{"key":"2023041304070583000_33.39.15388.4","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00923"},{"key":"2023041304070583000_33.39.15388.5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R400040200"},{"key":"2023041304070583000_33.39.15388.6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M706275200"},{"key":"2023041304070583000_33.39.15388.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2007.09.002"},{"key":"2023041304070583000_33.39.15388.8","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1242\/jcs.115.3.599","article-title":"pH-dependent regulation of lysosomal calcium in macrophages","volume":"115","author":"Christensen","year":"2002","journal-title":"J Cell Sci"},{"key":"2023041304070583000_33.39.15388.9","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(03)00130-0"},{"key":"2023041304070583000_33.39.15388.10","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.003285"},{"key":"2023041304070583000_33.39.15388.11","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0093"},{"key":"2023041304070583000_33.39.15388.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2009.01.025"},{"key":"2023041304070583000_33.39.15388.13","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(01)00235-2"},{"key":"2023041304070583000_33.39.15388.14","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0675"},{"key":"2023041304070583000_33.39.15388.15","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1876"},{"key":"2023041304070583000_33.39.15388.16","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.90402.2008"},{"key":"2023041304070583000_33.39.15388.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2012.03.012"},{"key":"2023041304070583000_33.39.15388.18","doi-asserted-by":"publisher","DOI":"10.2174\/138161208784480135"},{"key":"2023041304070583000_33.39.15388.19","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1523\/JNEUROSCI.22-05-01967.2002","article-title":"Blockade of striatal adenosine A2A receptor reduces, through a presynaptic mechanism, quinolinic-acid induced excitotoxicity: a possible relevance to neuroprotective interventions in neurodegenerative diseases of the striatum","volume":"22","author":"Popoli","year":"2002","journal-title":"J Neurosci"},{"key":"2023041304070583000_33.39.15388.20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.36.25833"},{"key":"2023041304070583000_33.39.15388.21","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awl260"},{"key":"2023041304070583000_33.39.15388.22","first-page":"2341","article-title":"Lysosomal Ca2+ stores in bovine corneal endothelium","volume":"43","author":"Srinivas","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2023041304070583000_33.39.15388.23","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0004.2003.00147.x"},{"key":"2023041304070583000_33.39.15388.24","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412898200"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.0558-13.2013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T21:50:12Z","timestamp":1681422612000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.0558-13.2013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,25]]},"references-count":24,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2013,9,25]]},"published-print":{"date-parts":[[2013,9,25]]}},"alternative-id":["10.1523\/JNEUROSCI.0558-13.2013"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.0558-13.2013","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,25]]}}}