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Neurosci."],"published-print":{"date-parts":[[2023,1,11]]},"abstract":"<jats:p>Lesion localization is the basis for understanding neurologic disease, which is predicated on neuroanatomical knowledge carefully cataloged from histology and imaging atlases. However, it is often difficult to correlate clinical images of brainstem injury obtained by MRI scans with the details of human brainstem neuroanatomy represented in atlases, which are mostly based on cytoarchitecture using Nissl stain or a single histochemical stain, and usually do not include the cerebellum. Here, we report a high-resolution (200 \u03bcm) 7T MRI of a cadaveric male human brainstem and cerebellum paired with detailed, coregistered histology (at 2 \u03bcm single-cell resolution) of the immunohistochemically stained cholinergic, serotonergic, and catecholaminergic (dopaminergic, noradrenergic, and adrenergic) neurons, in relationship to each other and to the cerebellum. These immunohistochemical findings provide novel insights into the spatial relationships of brainstem cell types and nuclei, including subpopulations of melanin and TH<jats:sup>+<\/jats:sup>neurons, and allows for more informed structural annotation of cell groups. Moreover, the coregistered MRI-paired histology helps validate imaging findings. This is useful for interpreting both scans and histology, and to understand the cell types affected by lesions. Our detailed chemoarchitecture and cytoarchitecture with corresponding high-resolution MRI builds on previous atlases of the human brainstem and cerebellum, and makes precise identification of brainstem and cerebellar cell groups involved in clinical lesions accessible for both laboratory scientists and clinicians alike.<\/jats:p><jats:p><jats:bold>SIGNIFICANCE STATEMENT<\/jats:bold>Clinicians and neuroscientists frequently use cross-sectional anatomy of the human brainstem from MRI scans for both clinical and laboratory investigations, but they must rely on brain atlases to neuroanatomical structures. Such atlases generally lack both detail of brainstem chemical cell types, and the cerebellum, which provides an important spatial reference. Our current atlas maps the distribution of key brainstem cell types (cholinergic, serotonergic, and catecholaminergic neurons) in relationship to each other and the cerebellum, and pairs this histology with 7T MR images from the identical brain. This atlas allows correlation of the chemoarchitecture with corresponding MRI, and makes the identification of cell groups that are often discussed, but rarely identifiable on MRI scan, accessible to clinicians and clinical researchers.<\/jats:p>","DOI":"10.1523\/jneurosci.0587-22.2022","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T18:50:25Z","timestamp":1669661425000},"page":"221-239","source":"Crossref","is-referenced-by-count":18,"title":["Human Brainstem and Cerebellum Atlas: Chemoarchitecture and Cytoarchitecture Paired to MRI"],"prefix":"10.1523","volume":"43","author":[{"given":"Lindsay J.","family":"Agostinelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott C.","family":"Seaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5788-3014","authenticated-orcid":false,"given":"Clifford B.","family":"Saper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dustin P.","family":"Fykstra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco M.","family":"Hefti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R.","family":"Koscik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Dlouhy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander G.","family":"Bassuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2022,11,28]]},"reference":[{"key":"2023041803552086000_43.2.221.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2021.118135"},{"key":"2023041803552086000_43.2.221.2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902730207"},{"key":"2023041803552086000_43.2.221.3","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902160106"},{"key":"2023041803552086000_43.2.221.4","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-53486-6.00069-7"},{"key":"2023041803552086000_43.2.221.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2010.09.025"},{"key":"2023041803552086000_43.2.221.6","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890070407"},{"key":"2023041803552086000_43.2.221.7","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902620408"},{"key":"2023041803552086000_43.2.221.8","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/BF00307185","article-title":"Nuclei of the human brain stem: V. 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