{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:42:23Z","timestamp":1790847743263,"version":"4.1.0"},"reference-count":56,"publisher":"Society for Neuroscience","issue":"21","license":[{"start":{"date-parts":[[2005,11,25]],"date-time":"2005-11-25T00:00:00Z","timestamp":1132876800000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2005,5,25]]},"abstract":"<jats:p>Retinoids participate in many essential processes including the initial event in photoreception. 11-<jats:italic>cis-<\/jats:italic>retinal binds to opsin and undergoes a light-driven isomerization to all-<jats:italic>trans-<\/jats:italic>retinal. In mammals, the all-<jats:italic>trans<\/jats:italic>-retinal is converted to vitamin A (all-<jats:italic>trans<\/jats:italic>-retinol) and is transported to the retinal pigment epithelium (RPE), where along with dietary vitamin A, it is converted into 11-<jats:italic>cis-<\/jats:italic>retinal. Although this cycle has been studied extensively in mammals, many questions remain, including the specific roles of retinoid-binding proteins. Here, we establish the<jats:italic>Drosophila<\/jats:italic>visual system as a genetic model for characterizing retinoid-binding proteins. In a genetic screen for mutations that affect the biosynthesis of rhodopsin, we identified a novel CRAL-TRIO domain protein, prolonged depolarization afterpotential is not apparent (PINTA), which binds to all-<jats:italic>trans<\/jats:italic>-retinol. We demonstrate that PINTA functions subsequent to the production of vitamin A and is expressed and required in the retinal pigment cells. These results represent the first genetic evidence for a role for the retinal pigment cells in the visual response. Moreover, our data implicate<jats:italic>Drosophila<\/jats:italic>retinal pigment cells as functioning in the conversion of dietary all-<jats:italic>trans<\/jats:italic>-retinol to 11-<jats:italic>cis-<\/jats:italic>retinal and suggest that these cells are the closest invertebrate equivalent to the RPE.<\/jats:p>","DOI":"10.1523\/jneurosci.0995-05.2005","type":"journal-article","created":{"date-parts":[[2005,5,25]],"date-time":"2005-05-25T21:23:52Z","timestamp":1117056232000},"page":"5187-5194","source":"Crossref","is-referenced-by-count":49,"title":["Rhodopsin Formation in<i>Drosophila<\/i>Is Dependent on the PINTA Retinoid-Binding Protein"],"prefix":"10.1523","volume":"25","author":[{"given":"Tao","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Montell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2005,5,25]]},"reference":[{"key":"2023041303124704000_25.21.5187.1","first-page":"17662","volume":"266","year":"1991","journal-title":"Retinoic acid-induced expression of CRABP-II but not CRABP-I in adult human skin in vivo and in skin fibroblasts in vitro. 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