{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:54:11Z","timestamp":1789080851039,"version":"build-2803163510"},"reference-count":68,"publisher":"Society for Neuroscience","issue":"4","license":[{"start":{"date-parts":[[2006,7,25]],"date-time":"2006-07-25T00:00:00Z","timestamp":1153785600000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2006,1,25]]},"abstract":"<jats:p>The central histaminergic neuron system inhibits epileptic seizures, which is suggested to occur mainly through histamine 1 (H<jats:sub>1<\/jats:sub>) and histamine 3 (H<jats:sub>3<\/jats:sub>) receptors. However, the importance of histaminergic neurons in seizure-induced cell damage is poorly known. In this study, we used an organotypic coculture system and confocal microscopy to examine whether histaminergic neurons, which were verified by immunohistochemistry, have any protective effect on kainic acid (KA)-induced neuronal damage in the developing hippocampus. Fluoro-Jade B, a specific marker for degenerating neurons, indicated that, after the 12 h KA (5 \u03bc<jats:sc>m<\/jats:sc>) treatment, neuronal damage was significantly attenuated in the hippocampus cultured together with the posterior hypothalamic slice containing histaminergic neurons [HI plus HY (POST)] when compared with the hippocampus cultured alone (HI) or with the anterior hypothalamus devoid of histaminergic neurons. Moreover, \u03b1-fluoromethylhistidine, an inhibitor of histamine synthesis, eliminated the neuroprotective effect in KA-treated HI plus HY (POST), and extracellularly applied histamine (1 n<jats:sc>m<\/jats:sc>to 100 \u03bc<jats:sc>m<\/jats:sc>) significantly attenuated neuronal damage only at 1 n<jats:sc>m<\/jats:sc>concentration in HI. After the 6 h KA treatment, spontaneous electrical activity registered in the CA1 subregion contained significantly less burst activity in HI plus HY (POST) than in HI. Finally, in KA-treated slices, the H<jats:sub>3<\/jats:sub>receptor antagonist thioperamide enhanced the neuroprotective effect of histaminergic neurons, whereas the H<jats:sub>1<\/jats:sub>receptor antagonists triprolidine and mepyramine dose-dependently decreased the neuroprotection in HI plus HY (POST). Our results suggest that histaminergic neurons protect the developing hippocampus from KA-induced neuronal damage, with regulation of neuronal survival being at least partly mediated through H<jats:sub>1<\/jats:sub>and H<jats:sub>3<\/jats:sub>receptors.<\/jats:p>","DOI":"10.1523\/jneurosci.1369-05.2006","type":"journal-article","created":{"date-parts":[[2006,1,25]],"date-time":"2006-01-25T20:28:04Z","timestamp":1138220884000},"page":"1088-1097","source":"Crossref","is-referenced-by-count":39,"title":["Histaminergic Neurons Protect the Developing Hippocampus from Kainic Acid-Induced Neuronal Damage in an Organotypic Coculture System"],"prefix":"10.1523","volume":"26","author":[{"given":"Tiina-Kaisa","family":"Kukko-Lukjanov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanna","family":"Soini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomi","family":"Taira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimmo A.","family":"Michelsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pertti","family":"Panula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irma E.","family":"Holopainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2006,1,25]]},"reference":[{"key":"2023041303163216000_26.4.1088.1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90259-P"},{"key":"2023041303163216000_26.4.1088.2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903230109"},{"key":"2023041303163216000_26.4.1088.3","doi-asserted-by":"publisher","DOI":"10.1038\/302832a0"},{"key":"2023041303163216000_26.4.1088.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(03)00141-6"},{"key":"2023041303163216000_26.4.1088.5","doi-asserted-by":"publisher","DOI":"10.1126\/science.8391168"},{"key":"2023041303163216000_26.4.1088.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01813-0"},{"key":"2023041303163216000_26.4.1088.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01659-3"},{"key":"2023041303163216000_26.4.1088.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.777ab.x"},{"key":"2023041303163216000_26.4.1088.9","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(94)00138-I"},{"key":"2023041303163216000_26.4.1088.10","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(95)90026-8"},{"key":"2023041303163216000_26.4.1088.11","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970901)385:3<385::AID-CNE4>3.0.CO;2-#"},{"key":"2023041303163216000_26.4.1088.12","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.10145"},{"key":"2023041303163216000_26.4.1088.13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb13234.x"},{"key":"2023041303163216000_26.4.1088.14","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00557-X"},{"key":"2023041303163216000_26.4.1088.15","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(99)00032-7"},{"key":"2023041303163216000_26.4.1088.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02627-5"},{"key":"2023041303163216000_26.4.1088.17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1997.tb01657.x"},{"key":"2023041303163216000_26.4.1088.18","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1523\/JNEUROSCI.04-05-01312.1984","volume":"4","year":"(1984)","journal-title":"J Neurosci"},{"key":"2023041303163216000_26.4.1088.19","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(94)00040-O"},{"key":"2023041303163216000_26.4.1088.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2003.08.009"},{"key":"2023041303163216000_26.4.1088.21","doi-asserted-by":"publisher","DOI":"10.1007\/BF00421114"},{"key":"2023041303163216000_26.4.1088.22","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903400408"},{"key":"2023041303163216000_26.4.1088.23","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1034"},{"key":"2023041303163216000_26.4.1088.24","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(03)00046-0"},{"key":"2023041303163216000_26.4.1088.25","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10005"},{"key":"2023041303163216000_26.4.1088.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2004.01.005"},{"key":"2023041303163216000_26.4.1088.27","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(01)00218-2"},{"key":"2023041303163216000_26.4.1088.28","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0006-8993(00)03041-9","volume":"887","year":"(2000)","journal-title":"Brain Res"},{"key":"2023041303163216000_26.4.1088.29","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5290.1133"},{"key":"2023041303163216000_26.4.1088.30","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1111\/j.1528-1157.1995.tb00996.x","volume":"36","year":"(1995)","journal-title":"Epilepsia"},{"key":"2023041303163216000_26.4.1088.31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231496298"},{"key":"2023041303163216000_26.4.1088.32","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)00050-2"},{"key":"2023041303163216000_26.4.1088.33","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-0618(03)00043-7"},{"key":"2023041303163216000_26.4.1088.34","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00168-3"},{"key":"2023041303163216000_26.4.1088.35","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.02.007"},{"key":"2023041303163216000_26.4.1088.36","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)03006-2"},{"key":"2023041303163216000_26.4.1088.37","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1152\/jn.2000.83.2.693","volume":"83","year":"(2000)","journal-title":"J Neurophysiol"},{"key":"2023041303163216000_26.4.1088.38","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0553-05.2005"},{"key":"2023041303163216000_26.4.1088.39","doi-asserted-by":"crossref","first-page":"4575","DOI":"10.1523\/JNEUROSCI.13-11-04575.1993","volume":"13","year":"(1993)","journal-title":"J Neurosci"},{"key":"2023041303163216000_26.4.1088.40","doi-asserted-by":"crossref","first-page":"6276","DOI":"10.1523\/JNEUROSCI.20-16-06276.2000","volume":"20","year":"(2000)","journal-title":"J Neurosci"},{"key":"2023041303163216000_26.4.1088.41","doi-asserted-by":"crossref","first-page":"S49","DOI":"10.1007\/PL00022442","volume":"51","year":"(2002)","journal-title":"Inflamm Res"},{"key":"2023041303163216000_26.4.1088.42","first-page":"13","volume":"14","year":"(1992)","journal-title":"Methods Find Exp Clin Pharmacol"},{"key":"2023041303163216000_26.4.1088.43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2572"},{"key":"2023041303163216000_26.4.1088.44","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90307-P"},{"key":"2023041303163216000_26.4.1088.45","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-1211(02)00076-1"},{"key":"2023041303163216000_26.4.1088.46","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00358-9"},{"key":"2023041303163216000_26.4.1088.47","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0920-1211(91)90003-X","volume":"10","year":"(1991)","journal-title":"Epilepsy Res"},{"key":"2023041303163216000_26.4.1088.48","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02513-0"},{"key":"2023041303163216000_26.4.1088.49","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01387-X"},{"key":"2023041303163216000_26.4.1088.50","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00008.x"},{"key":"2023041303163216000_26.4.1088.51","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0166-4328(01)00220-0","volume":"124","year":"(2001)","journal-title":"Behav Brain Res"},{"key":"2023041303163216000_26.4.1088.52","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-6376-8_7"},{"key":"2023041303163216000_26.4.1088.53","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(91)90128-M"},{"key":"2023041303163216000_26.4.1088.54","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(98)00152-3"},{"key":"2023041303163216000_26.4.1088.55","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.005652"},{"key":"2023041303163216000_26.4.1088.56","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1996.tb00042.x"},{"key":"2023041303163216000_26.4.1088.57","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(83)90308-7"},{"key":"2023041303163216000_26.4.1088.58","first-page":"610","volume":"38","year":"(1990)","journal-title":"Mol Pharmacol"},{"key":"2023041303163216000_26.4.1088.59","first-page":"289","volume":"19","year":"(1997)","journal-title":"Methods Find Exp Clin Pharmacol"},{"key":"2023041303163216000_26.4.1088.60","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)82012-5"},{"key":"2023041303163216000_26.4.1088.61","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.08.019"},{"key":"2023041303163216000_26.4.1088.62","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(01)00261-3"},{"key":"2023041303163216000_26.4.1088.63","doi-asserted-by":"publisher","DOI":"10.1007\/BF00167568"},{"key":"2023041303163216000_26.4.1088.64","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0014-2999(93)90717-V","volume":"234","year":"(1993)","journal-title":"Eur J Pharmacol"},{"key":"2023041303163216000_26.4.1088.65","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90005-1"},{"key":"2023041303163216000_26.4.1088.66","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/0304-3940(96)13078-0","volume":"217","year":"(1996)","journal-title":"Neurosci Lett"},{"key":"2023041303163216000_26.4.1088.67","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.ejphar.2003.09.066","volume":"482","year":"(2003)","journal-title":"Eur J Pharmacol"},{"key":"2023041303163216000_26.4.1088.68","doi-asserted-by":"crossref","first-page":"6052","DOI":"10.1523\/JNEUROSCI.22-14-06052.2002","volume":"22","year":"(2002)","journal-title":"J Neurosci"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.1369-05.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T17:12:35Z","timestamp":1681405955000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.1369-05.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,25]]},"references-count":68,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2006,1,25]]},"published-print":{"date-parts":[[2006,1,25]]}},"alternative-id":["10.1523\/JNEUROSCI.1369-05.2006"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.1369-05.2006","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,25]]}}}