{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T06:06:03Z","timestamp":1794377163457,"version":"4.9.0"},"reference-count":52,"publisher":"Society for Neuroscience","issue":"21","license":[{"start":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T00:00:00Z","timestamp":1322179200000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2011,5,25]]},"abstract":"<jats:p>\n                    Spiral ganglion neurons (SGNs) are postsynaptic to hair cells and project to the brainstem. The inner hair cell (IHC) to SGN synapse is susceptible to glutamate excitotoxicity and to acoustic trauma, with potentially adverse consequences to long-term SGN survival. We used a cochlear explant culture from P6 rat pups consisting of a portion of organ of Corti maintained intact with the corresponding portion of spiral ganglion to investigate excitotoxic damage to IHC\u2013SGN synapses\n                    <jats:italic>in vitro<\/jats:italic>\n                    . The normal innervation pattern is preserved\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Brief treatment with NMDA and kainate results in loss of IHC\u2013SGN synapses and degeneration of the distal type 1 SGN peripheral axons, mimicking damage to SGN peripheral axons caused by excitotoxicity or noise\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The number of IHC presynaptic ribbons is not significantly altered. Reinnervation of IHCs occurs and regenerating axons remain restricted to the IHC row. However, the number of postsynaptic densities (PSDs) does not fully recover and not all axons regrow to the IHCs. Addition of either neurotrophin-3 (NT-3) or BDNF increases axon growth and synaptogenesis. Selective blockade of endogenous NT-3 signaling with TrkC\u2013IgG reduced regeneration of axons and PSDs, but TrkB\u2013IgG, which blocks BDNF, has no such effect, indicating that endogenous NT-3 is necessary for SGN axon growth and synaptogenesis. Remarkably, TrkC\u2013IgG reduced axon growth and synaptogenesis even in the presence of BDNF, indicating that endogenous NT-3 has a distinctive role, not mimicked by BDNF, in promoting SGN axon growth in the organ of Corti and synaptogenesis on IHCs.\n                  <\/jats:p>","DOI":"10.1523\/jneurosci.1434-10.2011","type":"journal-article","created":{"date-parts":[[2011,5,25]],"date-time":"2011-05-25T12:49:08Z","timestamp":1306327748000},"page":"7938-7949","source":"Crossref","is-referenced-by-count":143,"title":["Functional Role of Neurotrophin-3 in Synapse Regeneration by Spiral Ganglion Neurons on Inner Hair Cells after Excitotoxic Trauma\n                    <i>In Vitro<\/i>"],"prefix":"10.1523","volume":"31","author":[{"given":"Qiong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven 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