{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:53:27Z","timestamp":1790153607256,"version":"4.0.1"},"reference-count":56,"publisher":"Society for Neuroscience","issue":"48","license":[{"start":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T00:00:00Z","timestamp":1464825600000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2015,12,2]]},"abstract":"<jats:p>\n                    Activation of taste buds triggers the release of several neurotransmitters, including ATP and serotonin (5-hydroxytryptamine; 5-HT). Type III taste cells release 5-HT directly in response to acidic (sour) stimuli and indirectly in response to bitter and sweet tasting stimuli. Although ATP is necessary for activation of nerve fibers for all taste stimuli, the role of 5-HT is unclear. We investigated whether gustatory afferents express functional 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    receptors and, if so, whether these receptors play a role in transmission of taste information from taste buds to nerves. In mice expressing GFP under the control of the 5-HT\n                    <jats:sub>3A<\/jats:sub>\n                    promoter, a subset of cells in the geniculate ganglion and nerve fibers in taste buds are GFP-positive. RT-PCR and\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization confirmed the presence of 5-HT\n                    <jats:sub>3A<\/jats:sub>\n                    mRNA in the geniculate ganglion. Functional studies show that only those geniculate ganglion cells expressing 5-HT\n                    <jats:sub>3A<\/jats:sub>\n                    -driven GFP respond to 10 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    5-HT and this response is blocked by 1 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    ondansetron, a 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    antagonist, and mimicked by application of 10 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    m-chlorophenylbiguanide, a 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    agonist. Pharmacological blockade of 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    receptors\n                    <jats:italic>in vivo<\/jats:italic>\n                    or genetic deletion of the 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    receptors reduces taste nerve responses to acids and other taste stimuli compared with controls, but only when urethane was used as the anesthetic. We find that anesthetic levels of pentobarbital reduce taste nerve responses apparently by blocking the 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    receptors. Our results suggest that 5-HT released from type III cells activates gustatory nerve fibers via 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    receptors, accounting for a significant proportion of the neural taste response.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>SIGNIFICANCE STATEMENT<\/jats:bold>\n                    Historically, serotonin (5-hydroxytryptamine; 5-HT) has been described as a candidate neurotransmitter in the gustatory system and recent studies show that type III taste receptor cells release 5-HT in response to various taste stimuli. In the present study, we demonstrate that a subset of gustatory sensory neurons express functional 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    receptors that play a significant role in the neurotransmission of taste information from taste buds to nerves. In addition, we show that the anesthetic pentobarbital, widely used in taste nerve recordings, blocks 5-HT\n                    <jats:sub>3<\/jats:sub>\n                    signaling. Therefore, many conclusions drawn from those data need to be reexamined in light of this anesthetic effect.\n                  <\/jats:p>","DOI":"10.1523\/jneurosci.1868-15.2015","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T12:22:23Z","timestamp":1449058943000},"page":"15984-15995","source":"Crossref","is-referenced-by-count":68,"title":["The Role of 5-HT\n                    <sub>3<\/sub>\n                    Receptors in Signaling from Taste Buds to Nerves"],"prefix":"10.1523","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2881-4861","authenticated-orcid":false,"given":"Eric D.","family":"Larson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurelie","family":"Vandenbeuch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Voigt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Meyerhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue C.","family":"Kinnamon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5923-5099","authenticated-orcid":false,"given":"Thomas E.","family":"Finger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2015,12,2]]},"reference":[{"key":"2023041304202320000_35.48.15984.1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1089\/gen.31.02.08","article-title":"Antibodies against membrane protein targets","volume":"31","author":"Banik Soma","year":"2011","journal-title":"Genet Eng Biotechnol News"},{"key":"2023041304202320000_35.48.15984.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(97)00059-2"},{"key":"2023041304202320000_35.48.15984.3","doi-asserted-by":"publisher","DOI":"10.1007\/s002100000288"},{"key":"2023041304202320000_35.48.15984.4","doi-asserted-by":"publisher","DOI":"10.1038\/nature13873"},{"key":"2023041304202320000_35.48.15984.5","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199904060-00037"},{"key":"2023041304202320000_35.48.15984.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00131-5"},{"key":"2023041304202320000_35.48.15984.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2011.01.010"},{"key":"2023041304202320000_35.48.15984.8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0515-06.2006"},{"key":"2023041304202320000_35.48.15984.9","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21494"},{"key":"2023041304202320000_35.48.15984.10","doi-asserted-by":"publisher","DOI":"10.1126\/science.1118435"},{"key":"2023041304202320000_35.48.15984.11","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900790107"},{"key":"2023041304202320000_35.48.15984.12","doi-asserted-by":"publisher","DOI":"10.1159\/000146320"},{"key":"2023041304202320000_35.48.15984.13","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4892(02)00212-6"},{"key":"2023041304202320000_35.48.15984.14","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(00)00130-2"},{"key":"2023041304202320000_35.48.15984.15","doi-asserted-by":"publisher","DOI":"10.1038\/nature13552"},{"key":"2023041304202320000_35.48.15984.16","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3459-06.2006"},{"key":"2023041304202320000_35.48.15984.17","doi-asserted-by":"publisher","DOI":"10.1007\/s002320001014"},{"key":"2023041304202320000_35.48.15984.18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020007"},{"key":"2023041304202320000_35.48.15984.19","doi-asserted-by":"publisher","DOI":"10.3109\/10799899109066399"},{"key":"2023041304202320000_35.48.15984.20","doi-asserted-by":"publisher","DOI":"10.1038\/nature05084"},{"key":"2023041304202320000_35.48.15984.21","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.151233"},{"key":"2023041304202320000_35.48.15984.22","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2351-09.2009"},{"key":"2023041304202320000_35.48.15984.23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025471"},{"key":"2023041304202320000_35.48.15984.24","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4446-04.2005"},{"key":"2023041304202320000_35.48.15984.25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611280104"},{"key":"2023041304202320000_35.48.15984.26","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22000"},{"key":"2023041304202320000_35.48.15984.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.07.069"},{"key":"2023041304202320000_35.48.15984.28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112152"},{"key":"2023041304202320000_35.48.15984.29","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.002311"},{"key":"2023041304202320000_35.48.15984.30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15314.x"},{"key":"2023041304202320000_35.48.15984.31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00572.2003"},{"key":"2023041304202320000_35.48.15984.32","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02292-7"},{"key":"2023041304202320000_35.48.15984.33","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902350105"},{"key":"2023041304202320000_35.48.15984.34","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bju028"},{"key":"2023041304202320000_35.48.15984.35","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bju066"},{"key":"2023041304202320000_35.48.15984.36","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00414.2010"},{"key":"2023041304202320000_35.48.15984.37","doi-asserted-by":"publisher","DOI":"10.1038\/nature726"},{"key":"2023041304202320000_35.48.15984.38","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(73)90002-6"},{"key":"2023041304202320000_35.48.15984.39","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90100-7"},{"key":"2023041304202320000_35.48.15984.40","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)91183-1"},{"key":"2023041304202320000_35.48.15984.41","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)90244-0"},{"key":"2023041304202320000_35.48.15984.42","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjs004"},{"key":"2023041304202320000_35.48.15984.43","doi-asserted-by":"publisher","DOI":"10.1038\/nature11905"},{"key":"2023041304202320000_35.48.15984.44","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601526"},{"key":"2023041304202320000_35.48.15984.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2012.12.002"},{"key":"2023041304202320000_35.48.15984.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.07.009"},{"key":"2023041304202320000_35.48.15984.47","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05397.x"},{"key":"2023041304202320000_35.48.15984.48","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-265355"},{"key":"2023041304202320000_35.48.15984.49","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1863-07.2007"},{"key":"2023041304202320000_35.48.15984.50","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900820306"},{"key":"2023041304202320000_35.48.15984.51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1309468110"},{"key":"2023041304202320000_35.48.15984.52","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.281014"},{"key":"2023041304202320000_35.48.15984.53","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(01)00384-8"},{"key":"2023041304202320000_35.48.15984.54","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9171"},{"key":"2023041304202320000_35.48.15984.55","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1372"},{"key":"2023041304202320000_35.48.15984.56","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1523\/JNEUROSCI.22-03-01010.2002","article-title":"The 5-HT3 subtype of serotonin receptor contributes to nociceptive processing via a novel subset of myelinated and unmyelinated nociceptors","volume":"22","author":"Zeitz","year":"2002","journal-title":"J Neurosci"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.1868-15.2015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T18:38:29Z","timestamp":1681411109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.1868-15.2015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,2]]},"references-count":56,"journal-issue":{"issue":"48","published-online":{"date-parts":[[2015,12,2]]},"published-print":{"date-parts":[[2015,12,2]]}},"alternative-id":["10.1523\/JNEUROSCI.1868-15.2015"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.1868-15.2015","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725982782.793517213","asserted-by":"object"}]},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,2]]}}}