{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T06:52:48Z","timestamp":1793170368586,"version":"4.5.0"},"reference-count":61,"publisher":"Society for Neuroscience","issue":"2","license":[{"start":{"date-parts":[[2000,7,15]],"date-time":"2000-07-15T00:00:00Z","timestamp":963619200000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2000,1,15]]},"abstract":"<jats:p>Many nicotinic acetylcholine receptors (nAChRs) expressed by central neurons are located at presynaptic nerve terminals. These receptors have high calcium permeability and exhibit strong inward rectification, two important physiological features that enable them to facilitate transmitter release. Previously, we showed that intracellular polyamines act as gating molecules to block neuronal nAChRs in a voltage-dependent manner, leading to inward rectification. Our goal is to identify the structural determinants that underlie the block by intracellular polyamines and govern calcium permeability of neuronal nAChRs. We hypothesize that two ring-like collections of negatively charged amino acids (cytoplasmic and intermediate rings) near the intracellular mouth of the pore mediate the interaction with intracellular polyamines and also influence calcium permeability. Using site-directed mutagenesis and electrophysiology on \u03b1<jats:sub>4<\/jats:sub>\u03b2<jats:sub>2<\/jats:sub>and \u03b1<jats:sub>3<\/jats:sub>\u03b2<jats:sub>4<\/jats:sub>receptors expressed in<jats:italic>Xenopus<\/jats:italic>oocytes, we observed that removing the five negative charges of the cytoplasmic ring had little effect on either inward rectification or calcium permeability. However, partial removal of negative charges of the intermediate ring diminished the high-affinity, voltage-dependent interaction between intracellular polyamines and the receptor, abolishing inward rectification. In addition, these nonrectifying mutant receptors showed a drastic reduction in calcium permeability. Our results indicate that the negatively charged glutamic acid residues at the intermediate ring form both a high-affinity binding site for intracellular polyamines and a selectivity filter for inflowing calcium ions; that is, a common site links inward rectification and calcium permeability of neuronal nAChRs. Physiologically, this molecular mechanism provides insight into how presynaptic nAChRs act to influence transmitter release.<\/jats:p>","DOI":"10.1523\/jneurosci.20-02-00529.2000","type":"journal-article","created":{"date-parts":[[2018,4,5]],"date-time":"2018-04-05T22:34:42Z","timestamp":1522967682000},"page":"529-541","source":"Crossref","is-referenced-by-count":71,"title":["A Molecular Link between Inward Rectification and Calcium Permeability of Neuronal Nicotinic Acetylcholine \u03b13\u03b24 and \u03b14\u03b22 Receptors"],"prefix":"10.1523","volume":"20","author":[{"given":"Ali Pejmun","family":"Haghighi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellis","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2000,1,15]]},"reference":[{"key":"2023041302440055000_20.2.529.1","first-page":"67","article-title":"Calcium 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