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During the 2 h infusion study,<jats:sup>13<\/jats:sup>C incorporation in the amino acid pools of glutamate and glutamine were measured with a 5 min time resolution. With a plasma concentration ([Lac]<jats:sub>P<\/jats:sub>) being in the 0.8\u20132.8 mmol\/L range, the tissue lactate concentration ([Lac]<jats:sub>B<\/jats:sub>) was assessed as well as the fractional contribution of lactate to brain energy metabolism (CMRlac). From the measured relationship between unidirectional lactate influx (<jats:italic>V<\/jats:italic><jats:sub>in<\/jats:sub>) and plasma and brain lactate concentrations, lactate transport constants were calculated using a reversible Michaelis\u2013Menten model. The results show that (1) in the physiological range, plasma lactate unidirectional transport (<jats:italic>V<\/jats:italic><jats:sub>in<\/jats:sub>) and concentration in tissue increase close to linearly with the lactate concentration in plasma; (2) the maximum potential contribution of plasma lactate to brain metabolism is 10% under basal plasma lactate conditions of \u223c1.0 mmol\/L and as much as 60% at supraphysiological plasma lactate concentrations when the transporters are saturated; (3) the half-saturation constant<jats:italic>K<\/jats:italic><jats:sub>T<\/jats:sub>is 5.1 \u00b1 2.7 mmol\/L and<jats:italic>V<\/jats:italic><jats:sub>MAX<\/jats:sub>is 0.40 \u00b1 0.13 \u03bcmol \u00b7 g<jats:sup>\u22121<\/jats:sup>\u00b7 min<jats:sup>\u22121<\/jats:sup>(68% confidence interval); and (4) the majority of plasma lactate is metabolized in neurons similar to 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