{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:06:15Z","timestamp":1792019175784,"version":"4.4.0"},"reference-count":37,"publisher":"Society for Neuroscience","issue":"2","license":[{"start":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T00:00:00Z","timestamp":1404777600000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2014,1,8]]},"abstract":"<jats:p>Corticotropin releasing factor (CRF) is the primary mediator of stress responses, and nociceptin\/orphanin FQ (N\/OFQ) plays an important role in the modulation of these stress responses. Thus, in this multidisciplinary study, we explored the relationship between the N\/OFQ and the CRF systems in response to stress. Using<jats:italic>in situ<\/jats:italic>hybridization (ISH), we assessed the effect of body restraint stress on the gene expression of CRF and N\/OFQ-related genes in various subdivisions of the amygdala, a critical brain structure involved in the modulation of stress response and anxiety-like behaviors. We found a selective upregulation of the NOP and downregulation of the CRF<jats:sub>1<\/jats:sub>receptor transcripts in the CeA and in the BLA after body restraint. Thus, we performed intracellular electrophysiological recordings of GABA<jats:sub>A<\/jats:sub>-mediated IPSPs in the central nucleus of the amygdala (CeA) to explore functional interactions between CRF and N\/OFQ systems in this brain region. Acute application of CRF significantly increased IPSPs in the CeA, and this enhancement was blocked by N\/OFQ. Importantly, in stress-restraint rats, baseline CeA GABAergic responses were elevated and N\/OFQ exerted a larger inhibition of IPSPs compared with unrestraint rats. The NOP antagonist [Nphe1]-nociceptin(1\u201313)NH2 increased the IPSP amplitudes in restraint rats but not in unrestraint rats, suggesting a functional recruitment of the N\/OFQ system after acute stress. Finally, we evaluated the anxiety-like response in rats subjected to restraint stress and nonrestraint rats after N\/OFQ microinjection into the CeA. Intra-CeA injections of N\/OFQ significantly and selectively reduced anxiety-like behavior in restraint rats in the elevated plus maze. These combined results demonstrate that acute stress increases N\/OFQ systems in the CeA and that N\/OFQ has antistress properties.<\/jats:p>","DOI":"10.1523\/jneurosci.2400-13.2014","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:54:10Z","timestamp":1389203650000},"page":"363-372","source":"Crossref","is-referenced-by-count":91,"title":["Restraint Stress Alters Nociceptin\/Orphanin FQ and CRF Systems in the Rat Central Amygdala: Significance for Anxiety-Like Behaviors"],"prefix":"10.1523","volume":"34","author":[{"given":"Roberto","family":"Ciccocioppo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giordano","family":"de Guglielmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita C.","family":"Hansson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Ubaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marsida","family":"Kallupi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen T.","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S.","family":"Oleata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Heilig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Roberto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2014,1,8]]},"reference":[{"key":"2023041304084198000_34.2.363.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1369-1600.2012.00466.x"},{"key":"2023041304084198000_34.2.363.2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200105080-00019"},{"key":"2023041304084198000_34.2.363.3","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-002-1020-7"},{"key":"2023041304084198000_34.2.363.4","doi-asserted-by":"crossref","first-page":"9445","DOI":"10.1523\/JNEUROSCI.23-28-09445.2003","article-title":"The bed nucleus is a neuroanatomical substrate for the anorectic effect of corticotropin-releasing factor and for its reversal by nociceptin\/orphanin FQ","volume":"23","author":"Ciccocioppo","year":"2003","journal-title":"J Neurosci"},{"key":"2023041304084198000_34.2.363.5","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-003-1645-1"},{"key":"2023041304084198000_34.2.363.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2011.10.032"},{"key":"2023041304084198000_34.2.363.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2008.02.004"},{"key":"2023041304084198000_34.2.363.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2010.01392.x"},{"key":"2023041304084198000_34.2.363.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2011.11.009"},{"key":"2023041304084198000_34.2.363.10","doi-asserted-by":"publisher","DOI":"10.1016\/0149-7634(94)90027-2"},{"key":"2023041304084198000_34.2.363.11","doi-asserted-by":"crossref","first-page":"6013","DOI":"10.1523\/JNEUROSCI.23-14-06013.2003","article-title":"c-fos reduces corticosterone-mediated effects on neurotrophic factor expression in the rat hippocampal CA1 region","volume":"23","author":"Hansson","year":"2003","journal-title":"J Neurosci"},{"key":"2023041304084198000_34.2.363.12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604419103"},{"key":"2023041304084198000_34.2.363.13","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2648.1994.20040660.x"},{"key":"2023041304084198000_34.2.363.14","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200006260-00026"},{"key":"2023041304084198000_34.2.363.15","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-052209-100430"},{"key":"2023041304084198000_34.2.363.16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb48286.x"},{"key":"2023041304084198000_34.2.363.17","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01411-8"},{"key":"2023041304084198000_34.2.363.18","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990419)406:4<503::AID-CNE7>3.0.CO;2-P"},{"key":"2023041304084198000_34.2.363.19","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/S0022-3565(25)23989-1","article-title":"Ethanol inhibits glutamatergic neurotransmission in nucleus accumbens neurons by multiple mechanisms","volume":"271","author":"Nie","year":"1994","journal-title":"J Pharmacol Exp Ther"},{"key":"2023041304084198000_34.2.363.20","doi-asserted-by":"publisher","DOI":"10.1210\/me.2003-0365"},{"key":"2023041304084198000_34.2.363.21","unstructured":"Paxinos G Watson C (1998) The rat brain in stereotaxic coordinates (Academic, San Diego)."},{"key":"2023041304084198000_34.2.363.22","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(85)90031-7"},{"key":"2023041304084198000_34.2.363.23","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(02)00778-5"},{"key":"2023041304084198000_34.2.363.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2007.07.010"},{"key":"2023041304084198000_34.2.363.25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601899103"},{"key":"2023041304084198000_34.2.363.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437926100"},{"key":"2023041304084198000_34.2.363.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2009.11.007"},{"key":"2023041304084198000_34.2.363.28","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-007-0985-7"},{"key":"2023041304084198000_34.2.363.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.09.026"},{"key":"2023041304084198000_34.2.363.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2009.07.028"},{"key":"2023041304084198000_34.2.363.31","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22737"},{"key":"2023041304084198000_34.2.363.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2007.11.023"},{"key":"2023041304084198000_34.2.363.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.05.031"},{"key":"2023041304084198000_34.2.363.34","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00228-5"},{"key":"2023041304084198000_34.2.363.35","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199709080-00035"},{"key":"2023041304084198000_34.2.363.36","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-008-0183-3"},{"key":"2023041304084198000_34.2.363.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2009.02.014"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.2400-13.2014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T12:00:36Z","timestamp":1746100836000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.2400-13.2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,8]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,1,8]]},"published-print":{"date-parts":[[2014,1,8]]}},"alternative-id":["10.1523\/JNEUROSCI.2400-13.2014"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.2400-13.2014","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,8]]}}}