{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:09:09Z","timestamp":1761919749578},"reference-count":29,"publisher":"Society for Neuroscience","issue":"44","license":[{"start":{"date-parts":[[2014,4,30]],"date-time":"2014-04-30T00:00:00Z","timestamp":1398816000000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2013,10,30]]},"abstract":"<jats:p>Ca<jats:sup>2+<\/jats:sup>-dependent activator protein for secretion 1 (CAPS1) plays a regulatory role in the dense-core vesicle (DCV) exocytosis pathway, but its functions at the cellular and synaptic levels in the brain are essentially unknown because of neonatal death soon after birth in<jats:italic>Caps1<\/jats:italic>knock-out mice. To clarify the functions of the protein in the brain, we generated two conditional knock-out (cKO) mouse lines: 1) one lacking<jats:italic>Caps1<\/jats:italic>in the forebrain; and 2) the other lacking<jats:italic>Caps1<\/jats:italic>in the cerebellum. Both cKO mouse lines were born normally and grew to adulthood, although they showed subcellular and synaptic abnormalities. Forebrain-specific<jats:italic>Caps1<\/jats:italic>cKO mice showed reduced immunoreactivity for the DCV marker secretogranin II (SgII) and the<jats:italic>trans<\/jats:italic>-Golgi network (TGN) marker syntaxin 6, a reduced number of presynaptic DCVs, and dilated<jats:italic>trans<\/jats:italic>-Golgi cisternae in the CA3 region. Cerebellum-specific<jats:italic>Caps1<\/jats:italic>cKO mice had decreased immunoreactivity for SgII and brain-derived neurotrophic factor (BDNF) along the climbing fibers. At climbing fiber\u2013Purkinje cell synapses, the number of DCVs was markedly lower and the number of synaptic vesicles was also reduced. Correspondingly, the mean amplitude of EPSCs was decreased, whereas paired-pulse depression was significantly increased. Our results suggest that loss of CAPS1 disrupts the TGN\u2013DCV pathway, which possibly impairs synaptic transmission by reducing the presynaptic release probability.<\/jats:p>","DOI":"10.1523\/jneurosci.2777-13.2013","type":"journal-article","created":{"date-parts":[[2013,10,30]],"date-time":"2013-10-30T16:49:11Z","timestamp":1383151751000},"page":"17326-17334","source":"Crossref","is-referenced-by-count":22,"title":["CAPS1 Deficiency Perturbs Dense-Core Vesicle Trafficking and Golgi Structure and Reduces Presynaptic Release Probability in the Mouse Brain"],"prefix":"10.1523","volume":"33","author":[{"given":"Tetsushi","family":"Sadakata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Kakegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yo","family":"Shinoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayu","family":"Hosono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritsuko","family":"Katoh-Semba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Sekine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuji","family":"Iwasato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeyoshi","family":"Itohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Furuyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiya","family":"Kawaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuki","family":"Ishizaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michisuke","family":"Yuzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teiichi","family":"Furuichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"393","published-online":{"date-parts":[[2013,10,30]]},"reference":[{"key":"2023041304074010000_33.44.17326.1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.32.19637"},{"key":"2023041304074010000_33.44.17326.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80521-8"},{"key":"2023041304074010000_33.44.17326.3","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1523\/JNEUROSCI.17-07-02295.1997","article-title":"Distribution of brain-derived neurotrophic factor (BDNF) protein and mRNA in the normal adult rat CNS: evidence for anterograde axonal transport","volume":"17","author":"Conner","year":"1997","journal-title":"J Neurosci"},{"key":"2023041304074010000_33.44.17326.4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703699200"},{"key":"2023041304074010000_33.44.17326.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.07.028"},{"key":"2023041304074010000_33.44.17326.6","doi-asserted-by":"publisher","DOI":"10.1038\/35021059"},{"key":"2023041304074010000_33.44.17326.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.002"},{"key":"2023041304074010000_33.44.17326.8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2553-07.2008"},{"key":"2023041304074010000_33.44.17326.9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69010034.x"},{"key":"2023041304074010000_33.44.17326.10","doi-asserted-by":"publisher","DOI":"10.1038\/ng959"},{"key":"2023041304074010000_33.44.17326.11","first-page":"385","article-title":"Strain difference in establishment of mouse embryonic stem (ES) cell lines","volume":"38","author":"Kawase","year":"1994","journal-title":"Int J Dev Biol"},{"key":"2023041304074010000_33.44.17326.12","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.23.3752"},{"key":"2023041304074010000_33.44.17326.13","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5672-07.2008"},{"key":"2023041304074010000_33.44.17326.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5610-03.2004"},{"key":"2023041304074010000_33.44.17326.15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605699104"},{"key":"2023041304074010000_33.44.17326.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00382-8"},{"key":"2023041304074010000_33.44.17326.17","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2528-03.2004"},{"key":"2023041304074010000_33.44.17326.18","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20947"},{"key":"2023041304074010000_33.44.17326.19","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2279-06.2007"},{"key":"2023041304074010000_33.44.17326.20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29031"},{"key":"2023041304074010000_33.44.17326.21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.137414"},{"key":"2023041304074010000_33.44.17326.22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08431.x"},{"key":"2023041304074010000_33.44.17326.23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304727200"},{"key":"2023041304074010000_33.44.17326.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.02.019"},{"key":"2023041304074010000_33.44.17326.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.11.009"},{"key":"2023041304074010000_33.44.17326.26","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80522-X"},{"key":"2023041304074010000_33.44.17326.27","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90310-9"},{"key":"2023041304074010000_33.44.17326.28","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1423-07.2007"},{"key":"2023041304074010000_33.44.17326.29","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.64.092501.114547"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.2777-13.2013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T21:51:59Z","timestamp":1681422719000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.2777-13.2013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,30]]},"references-count":29,"journal-issue":{"issue":"44","published-online":{"date-parts":[[2013,10,30]]},"published-print":{"date-parts":[[2013,10,30]]}},"alternative-id":["10.1523\/JNEUROSCI.2777-13.2013"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.2777-13.2013","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,30]]}}}