{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:48:50Z","timestamp":1791413330869,"version":"4.3.3"},"reference-count":38,"publisher":"Society for Neuroscience","issue":"39","license":[{"start":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T00:00:00Z","timestamp":1364256000000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2012,9,26]]},"abstract":"<jats:p>Signaling through GABA<jats:sub>A<\/jats:sub>receptors controls neural progenitor cell (NPC) development<jats:italic>in vitro<\/jats:italic>and is altered in schizophrenic and autistic individuals. However, the<jats:italic>in vivo<\/jats:italic>function of GABA<jats:sub>A<\/jats:sub>signaling on neural stem cell proliferation, and ultimately neurogenesis, remains unknown. To examine GABA<jats:sub>A<\/jats:sub>function<jats:italic>in vivo<\/jats:italic>, we electroporated plasmids encoding short-hairpin (sh) RNA against the Na-K-2Cl cotransporter NKCC1 (shNKCC1) in NPCs of the neonatal subventricular zone in mice to reduce GABA<jats:sub>A<\/jats:sub>-induced depolarization. Reduced GABA<jats:sub>A<\/jats:sub>depolarization identified by a loss of GABA<jats:sub>A<\/jats:sub>-induced calcium responses in most electroporated NPCs led to a 70% decrease in the number of proliferative Ki67<jats:sup>+<\/jats:sup>NPCs and a 60% reduction in newborn neuron density. Premature loss of GABA<jats:sub>A<\/jats:sub>depolarization in newborn neurons resulted in truncated dendritic arborization at the time of synaptic integration. However, by 6 weeks the dendritic tree had partially recovered and displayed a small, albeit significant, decrease in dendritic complexity but not total dendritic length. To further examine GABA<jats:sub>A<\/jats:sub>function on NPCs, we treated animals with a GABA<jats:sub>A<\/jats:sub>allosteric agonist, pentobarbital. Enhancement of GABA<jats:sub>A<\/jats:sub>activity in NPCs increased the number of proliferative NPCs by 60%. Combining shNKCC1 and pentobarbital prevented the shNKCC1 and the pentobarbital effects on NPC proliferation, suggesting that these manipulations affected NPCs through GABA<jats:sub>A<\/jats:sub>receptors. Thus, dysregulation in GABA<jats:sub>A<\/jats:sub>depolarizing activity delayed dendritic development and reduced NPC proliferation resulting in decreased neuronal density.<\/jats:p>","DOI":"10.1523\/jneurosci.2864-12.2012","type":"journal-article","created":{"date-parts":[[2012,9,27]],"date-time":"2012-09-27T07:04:43Z","timestamp":1348729483000},"page":"13630-13638","source":"Crossref","is-referenced-by-count":71,"title":["NKCC1 Knockdown Decreases Neuron Production through GABA<sub>A<\/sub>-Regulated Neural Progenitor Proliferation and Delays Dendrite Development"],"prefix":"10.1523","volume":"32","author":[{"given":"Stephanie Z.","family":"Young","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Morgan","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Ikeda-Matsuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathryn","family":"Kubera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lique","family":"Bordey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2012,9,26]]},"reference":[{"key":"2023041303585377000_32.39.13630.1","doi-asserted-by":"publisher","DOI":"10.1001\/archgenpsychiatry.2010.114"},{"key":"2023041303585377000_32.39.13630.2","doi-asserted-by":"crossref","first-page":"722","DOI":"10.4161\/cc.5.7.2614","article-title":"Adult neurogenesis: basic concepts of signaling","volume":"5","author":"Bordey","year":"2006","journal-title":"Cell Cycle"},{"key":"2023041303585377000_32.39.13630.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2006.07.004"},{"key":"2023041303585377000_32.39.13630.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2008.11.048"},{"key":"2023041303585377000_32.39.13630.5","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E08-05-0478"},{"key":"2023041303585377000_32.39.13630.6","doi-asserted-by":"publisher","DOI":"10.1002\/stem.573"},{"key":"2023041303585377000_32.39.13630.7","doi-asserted-by":"publisher","DOI":"10.1038\/nature09582"},{"key":"2023041303585377000_32.39.13630.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2011.07782.x"},{"key":"2023041303585377000_32.39.13630.9","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4508-06.2006"},{"key":"2023041303585377000_32.39.13630.10","doi-asserted-by":"publisher","DOI":"10.1038\/nature04404"},{"key":"2023041303585377000_32.39.13630.11","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00497-5"},{"key":"2023041303585377000_32.39.13630.12","doi-asserted-by":"publisher","DOI":"10.2174\/187152708784083812"},{"key":"2023041303585377000_32.39.13630.13","first-page":"43","article-title":"Imaging and recording subventricular zone progenitor cells in live tissue of postnatal mice","volume":"4","author":"Lacar","year":"2010","journal-title":"Front Neurosci"},{"key":"2023041303585377000_32.39.13630.14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2011.07901.x"},{"key":"2023041303585377000_32.39.13630.15","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1114-05.2005"},{"key":"2023041303585377000_32.39.13630.16","doi-asserted-by":"publisher","DOI":"10.1038\/nn1522"},{"key":"2023041303585377000_32.39.13630.17","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20392"},{"key":"2023041303585377000_32.39.13630.18","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90008-X"},{"key":"2023041303585377000_32.39.13630.19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610155104"},{"key":"2023041303585377000_32.39.13630.20","doi-asserted-by":"publisher","DOI":"10.1542\/peds.113.5.e472"},{"key":"2023041303585377000_32.39.13630.21","doi-asserted-by":"publisher","DOI":"10.3109\/10673229.2010.511060"},{"key":"2023041303585377000_32.39.13630.22","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2298"},{"key":"2023041303585377000_32.39.13630.23","doi-asserted-by":"publisher","DOI":"10.1038\/nrn919"},{"key":"2023041303585377000_32.39.13630.24","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20576"},{"key":"2023041303585377000_32.39.13630.25","doi-asserted-by":"crossref","first-page":"6106","DOI":"10.1523\/JNEUROSCI.22-14-06106.2002","article-title":"Maturation and death of adult-born olfactory bulb granule neurons: role of olfaction","volume":"22","author":"Petreanu","year":"2002","journal-title":"J Neurosci"},{"key":"2023041303585377000_32.39.13630.26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05367.x"},{"key":"2023041303585377000_32.39.13630.27","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20735"},{"key":"2023041303585377000_32.39.13630.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.03.009"},{"key":"2023041303585377000_32.39.13630.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2010.02.004"},{"key":"2023041303585377000_32.39.13630.30","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.155879"},{"key":"2023041303585377000_32.39.13630.31","doi-asserted-by":"publisher","DOI":"10.1093\/schbul\/sbn155"},{"key":"2023041303585377000_32.39.13630.32","doi-asserted-by":"publisher","DOI":"10.1034\/j.1601-183X.2003.00037.x"},{"key":"2023041303585377000_32.39.13630.33","doi-asserted-by":"publisher","DOI":"10.1038\/nature08826"},{"key":"2023041303585377000_32.39.13630.34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403954101"},{"key":"2023041303585377000_32.39.13630.35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5599-07.2008"},{"key":"2023041303585377000_32.39.13630.36","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.042572"},{"key":"2023041303585377000_32.39.13630.37","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00002.2009"},{"key":"2023041303585377000_32.39.13630.38","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2010.00008"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.2864-12.2012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T21:15:18Z","timestamp":1681420518000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.2864-12.2012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,26]]},"references-count":38,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2012,9,26]]},"published-print":{"date-parts":[[2012,9,26]]}},"alternative-id":["10.1523\/JNEUROSCI.2864-12.2012"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.2864-12.2012","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,26]]}}}