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Archaerhodopsin-(Arch)-transduced RTN neurons were reversibly silenced by light in anesthetized rats. We bilaterally transduced RTN and nearby C1 neurons with Arch (PRSx8-ArchT-EYFP-LVV) and measured the cardiorespiratory consequences of Arch activation (10 s) in conscious rats during normoxia, hypoxia, or hyperoxia. RTN photoinhibition reduced breathing equally during non-REM sleep and quiet wake. Compared with normoxia, the breathing frequency reduction (\u0394f<jats:sub>R<\/jats:sub>) was larger in hyperoxia (65% FiO<jats:sub>2<\/jats:sub>), smaller in 15% FiO<jats:sub>2<\/jats:sub>, and absent in 12% FiO<jats:sub>2<\/jats:sub>. Tidal volume changes (\u0394V<jats:sub>T<\/jats:sub>) followed the same trend. The effect of hypoxia on \u0394f<jats:sub>R<\/jats:sub>was not arousal-dependent but was reversed by reacidifying the blood (acetazolamide; 3% FiCO<jats:sub>2<\/jats:sub>). \u0394f<jats:sub>R<\/jats:sub>was highly correlated with arterial pH up to arterial pH (pHa) 7.5 with no frequency inhibition occurring above pHa 7.53. Blood pressure was minimally reduced suggesting that C1 neurons were very modestly inhibited. In conclusion, RTN neurons regulate eupneic breathing about equally during both sleep and wake. RTN neurons are the first putative CRCs demonstrably silenced by hypocapnic hypoxia in conscious mammals. RTN neurons are silent above pHa 7.5 and increasingly active below this value. During hyperoxia, RTN activation maintains breathing despite the inactivity of the carotid bodies. Finally, during hypocapnic hypoxia, carotid body stimulation increases breathing frequency via pathways that bypass RTN.<\/jats:p>","DOI":"10.1523\/jneurosci.2923-14.2015","type":"journal-article","created":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T17:43:24Z","timestamp":1421257404000},"page":"527-543","source":"Crossref","is-referenced-by-count":73,"title":["Hypoxia Silences Retrotrapezoid Nucleus Respiratory Chemoreceptors via Alkalosis"],"prefix":"10.1523","volume":"35","author":[{"given":"Tyler M.","family":"Basting","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter G.R.","family":"Burke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy","family":"Kanbar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth 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