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We show that \u03b2-actin's 3\u2032-UTR can drive axonal localization of<jats:italic>GFP<\/jats:italic>mRNA in mature DRG neurons, but mice with \u03b3-<jats:italic>actin's<\/jats:italic>3\u2032-UTR show no axonal<jats:italic>GFP<\/jats:italic>mRNA. Peripheral axotomy triggers transport of the \u03b2-<jats:italic>actin 3\u2032-UTR<\/jats:italic>containing transgene mRNA into axons. This<jats:italic>GFP-3\u2032-<\/jats:italic>\u03b2-<jats:italic>actin<\/jats:italic>mRNA accumulates in injured PNS axons before activation of the transgene promoter peaks in the DRG. Spinal cord injury also increases axonal GFP signals in mice carrying this transgene without any increase in transgene expression in the DRGs. These data show for the first time that the \u03b2-<jats:italic>actin<\/jats:italic>3\u2032-UTR is sufficient for axonal localization in both PNS and CNS neurons<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1523\/jneurosci.2950-11.2011","type":"journal-article","created":{"date-parts":[[2011,10,12]],"date-time":"2011-10-12T17:02:13Z","timestamp":1318438933000},"page":"14481-14487","source":"Crossref","is-referenced-by-count":70,"title":["Axonal Localization of Transgene mRNA in Mature PNS and CNS Neurons"],"prefix":"10.1523","volume":"31","author":[{"given":"Dianna E.","family":"Willis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Donnelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chhavy","family":"Tep","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin","family":"Kendall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Erenstheyn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur W.","family":"English","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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