{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T23:56:40Z","timestamp":1785542200652,"version":"3.56.0"},"reference-count":70,"publisher":"Society for Neuroscience","issue":"50","license":[{"start":{"date-parts":[[2014,6,11]],"date-time":"2014-06-11T00:00:00Z","timestamp":1402444800000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2013,12,11]]},"abstract":"<jats:p>Bone morphogenic protein-4 (BMP4) and fibroblast growth factor-8 (FGF8) are thought to have opposite roles in defining epithelial versus neurogenic fate in the developing olfactory\/vomeronasal system. In particular, FGF8 has been implicated in specification of olfactory and gonadotropin releasing hormone-1 (GnRH) neurons, as well as in controlling olfactory stem cell survival. Using different knock-in mouse lines and Cre-lox-mediated lineage tracing,<jats:italic>Fgf8<\/jats:italic>expression and cell lineage was analyzed in the developing nose in relation to the expression of<jats:italic>Bmp4<\/jats:italic>and its antagonist<jats:italic>Noggin<\/jats:italic>(<jats:italic>Nog<\/jats:italic>). FGF8 is expressed by cells that acquire an epidermal, respiratory cell fate and not by stem cells that acquire neuronal olfactory or vomeronasal cell fate. Ectodermal and mesenchymal sources of BMP4 control the expression of BMP\/TGF\u03b2 antagonist<jats:italic>Nog<\/jats:italic>, whereas mesenchymal sources of<jats:italic>Nog<\/jats:italic>define the neurogenic borders of the olfactory pit.<jats:italic>Fgf8<\/jats:italic>hypomorph mouse models,<jats:italic>Fgf8<\/jats:italic><jats:sup>neo\/neo<\/jats:sup>and<jats:italic>Fgf8<\/jats:italic><jats:sup>neo\/null<\/jats:sup>, displayed severe craniofacial defects together with overlapping defects in the olfactory pit including (1) lack of neuronal formation ventrally, where GnRH neurons normally form, and (2) altered expression of<jats:italic>Bmp4<\/jats:italic>and<jats:italic>Nog<\/jats:italic>, with<jats:italic>Nog<\/jats:italic>ectopically expressed in the nasal mesenchyme and no longer defining the GnRH and vomeronasal neurogenic border. Together our data show that (1) FGF8 is not sufficient to induce ectodermal progenitors of the olfactory pit to acquire neural fate and (2) altered neurogenesis and lack of GnRH neuron specification after chronically reduced<jats:italic>Fgf8<\/jats:italic>expression reflected dysgenesis of the nasal region and loss of a specific neurogenic permissive milieu that was defined by mesenchymal signals.<\/jats:p>","DOI":"10.1523\/jneurosci.3238-13.2013","type":"journal-article","created":{"date-parts":[[2013,12,11]],"date-time":"2013-12-11T17:39:36Z","timestamp":1386783576000},"page":"19620-19634","source":"Crossref","is-referenced-by-count":49,"title":["The Indirect Role of Fibroblast Growth Factor-8 in Defining Neurogenic Niches of the Olfactory\/GnRH Systems"],"prefix":"10.1523","volume":"33","author":[{"given":"Paolo 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