{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:08:39Z","timestamp":1790017719542,"version":"4.0.1"},"reference-count":47,"publisher":"Society for Neuroscience","issue":"15","license":[{"start":{"date-parts":[[2015,10,15]],"date-time":"2015-10-15T00:00:00Z","timestamp":1444867200000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2015,4,15]]},"abstract":"<jats:p>PET imaging of nicotinic acetylcholine receptors (nAChRs) could become an effective tool for the diagnosis and therapy evaluation of neurologic diseases. Despite this, the role of nAChRs \u03b14\u03b22 receptors after brain diseases such as cerebral ischemia and its involvement in inflammatory reaction is still largely unknown. To investigate this, we performed in parallel<jats:italic>in vivo<\/jats:italic>magnetic resonance imaging (MRI) and positron emission tomography (PET) with 2[<jats:sup>18<\/jats:sup>F]-fluoro-A85380 and [<jats:sup>11<\/jats:sup>C]PK11195 at 1, 3, 7, 14, 21, and 28 d after middle cerebral artery occlusion (MCAO) in rats. In the ischemic territory, PET with 2[<jats:sup>18<\/jats:sup>F]-fluoro-A85380 and [<jats:sup>11<\/jats:sup>C]PK11195 showed a progressive binding increase from days 3\u20137, followed by a progressive decrease from days 14\u201328 after cerebral ischemia onset.<jats:italic>Ex vivo<\/jats:italic>immunohistochemistry for the nicotinic \u03b14\u03b22 receptor and the mitochondrial translocator protein (18 kDa) (TSPO) confirmed the PET findings and demonstrated the overexpression of \u03b14\u03b22 receptors in both microglia\/macrophages and astrocytes from days 7\u201328 after experimental ischemic stroke. Likewise, the role played by \u03b14\u03b22 receptors on neuroinflammation was supported by the increase of [<jats:sup>11<\/jats:sup>C]PK11195 binding in ischemic rats treated with the \u03b14\u03b22 antagonist dihydro-\u03b2-erythroidine hydrobromide (DHBE) at day 7 after MCAO. Finally, both functional and behavioral testing showed major impaired outcome at day 1 after ischemia onset, followed by a recovery of the sensorimotor function and dexterity from days 21\u201328 after experimental stroke. Together, these results suggest that the nicotinic \u03b14\u03b22 receptor could have a key role in the inflammatory reaction underlying cerebral ischemia in rats.<\/jats:p>","DOI":"10.1523\/jneurosci.3670-14.2015","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T16:37:33Z","timestamp":1429115853000},"page":"5998-6009","source":"Crossref","is-referenced-by-count":39,"title":["<i>In Vivo<\/i>PET Imaging of the \u03b14\u03b22 Nicotinic Acetylcholine Receptor As a Marker for Brain Inflammation after Cerebral 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