{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:59:20Z","timestamp":1773309560101,"version":"3.50.1"},"reference-count":41,"publisher":"Society for Neuroscience","issue":"1","license":[{"start":{"date-parts":[[2008,7,2]],"date-time":"2008-07-02T00:00:00Z","timestamp":1214956800000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2008,1,2]]},"abstract":"<jats:p>Circadian pacemaker circuits consist of ensembles of neurons, each expressing molecular oscillations, but how circuit-wide coordination of multiple oscillators regulates rhythmic physiological and behavioral outputs remains an open question. To investigate the relationship between the pattern of oscillator phase throughout the circadian pacemaker circuit and locomotor activity rhythms in<jats:italic>Drosophila<\/jats:italic>, we perturbed the electrical activity and pigment dispersing factor (PDF) levels of the lateral ventral neurons (LNv) and assayed their combinatorial effect on molecular oscillations in different parts of the circuit and on locomotor activity behavior. Altered electrical activity of PDF-expressing LNv causes initial behavioral arrhythmicity followed by gradual long-term emergence of two concurrent short- and long-period circadian behavioral activity bouts in \u223c60% of flies. Initial desynchrony of circuit-wide molecular oscillations is followed by the emergence of a novel pattern of period (PER) synchrony whereby two subgroups of dorsal neurons (DN1 and DN2) exhibit PER oscillation peaks coinciding with two activity bouts, whereas other neuronal subgroups exhibit a single PER peak coinciding with one of the two activity bouts. The emergence of this novel pattern of circuit-wide oscillator synchrony is not accompanied by concurrent change in the electrical activity of the LNv. In PDF-null flies, altered electrical activity of LNv drives a short-period circadian activity bout only, indicating that PDF-independent factors underlie the short-period circadian activity component and that the long-period circadian component is PDF-dependent. Thus, polyrhythmic behavioral patterns in electrically manipulated flies are regulated by circuit-wide coordination of molecular oscillations and electrical activity of LNv via PDF-dependent and -independent factors.<\/jats:p>","DOI":"10.1523\/jneurosci.4087-07.2008","type":"journal-article","created":{"date-parts":[[2008,1,2]],"date-time":"2008-01-02T16:43:15Z","timestamp":1199292195000},"page":"217-227","source":"Crossref","is-referenced-by-count":59,"title":["Pigment Dispersing Factor-Dependent and -Independent Circadian Locomotor Behavioral Rhythms"],"prefix":"10.1523","volume":"28","author":[{"given":"Vasu","family":"Sheeba","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay K.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaiyu","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Ting","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane K.","family":"O'Dowd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd C.","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"393","published-online":{"date-parts":[[2008,1,2]]},"reference":[{"key":"2023041303292809000_28.1.217.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606870104"},{"key":"2023041303292809000_28.1.217.2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816x.2000.01450.x"},{"key":"2023041303292809000_28.1.217.3","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1242\/dev.118.2.401","article-title":"Targeted gene expression as a means of altering cell fates and generating dominant phenotypes","volume":"118","author":"Brand","year":"1993","journal-title":"Development"},{"key":"2023041303292809000_28.1.217.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608260104"},{"key":"2023041303292809000_28.1.217.5","doi-asserted-by":"publisher","DOI":"10.1038\/nature02935"},{"key":"2023041303292809000_28.1.217.6","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4109-05.2006"},{"key":"2023041303292809000_28.1.217.7","doi-asserted-by":"publisher","DOI":"10.3109\/01677068609106857"},{"key":"2023041303292809000_28.1.217.8","doi-asserted-by":"publisher","DOI":"10.1007\/s003590050192"},{"key":"2023041303292809000_28.1.217.9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3032.1983.tb00358.x"},{"key":"2023041303292809000_28.1.217.10","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1523\/JNEUROSCI.20-09-03339.2000","article-title":"Ectopic expression of the neuropeptide pigment-dispersing factor alters behavioral rhythms in Drosophila melanogaster","volume":"20","author":"Helfrich-F\u00f6rster","year":"2000","journal-title":"J Neurosci"},{"key":"2023041303292809000_28.1.217.11","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21146"},{"key":"2023041303292809000_28.1.217.12","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80877-6"},{"key":"2023041303292809000_28.1.217.13","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000619)422:1<66::AID-CNE5>3.0.CO;2-2"},{"key":"2023041303292809000_28.1.217.14","doi-asserted-by":"crossref","first-page":"6745","DOI":"10.1523\/JNEUROSCI.17-17-06745.1997","article-title":"Spatial and temporal expression of the period and timeless genes in the developing nervous system of Drosophila: newly identified pacemaker candidates and novel features of clock gene product cycling","volume":"17","author":"Kaneko","year":"1997","journal-title":"J Neurosci"},{"key":"2023041303292809000_28.1.217.15","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2370-04.2004"},{"key":"2023041303292809000_28.1.217.16","doi-asserted-by":"publisher","DOI":"10.1177\/0748730407299344"},{"key":"2023041303292809000_28.1.217.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.01.034"},{"key":"2023041303292809000_28.1.217.18","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00737-7"},{"key":"2023041303292809000_28.1.217.19","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20053"},{"key":"2023041303292809000_28.1.217.20","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3915-05.2006"},{"key":"2023041303292809000_28.1.217.21","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00117.2006"},{"key":"2023041303292809000_28.1.217.22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.070036197"},{"key":"2023041303292809000_28.1.217.23","doi-asserted-by":"crossref","first-page":"E13","DOI":"10.1371\/journal.pbio.0000013","article-title":"Drosophila free-running rhythms require intercellular communication","volume":"1","author":"Peng","year":"2003","journal-title":"PLoS Biol"},{"key":"2023041303292809000_28.1.217.24","doi-asserted-by":"crossref","first-page":"4087","DOI":"10.1523\/JNEUROSCI.17-11-04087.1997","article-title":"Pigment-dispersing hormone shifts the phase of the circadian pacemaker of the cockroach Leucophaea maderae","volume":"17","author":"Petri","year":"1997","journal-title":"J Neurosci"},{"key":"2023041303292809000_28.1.217.25","doi-asserted-by":"publisher","DOI":"10.1007\/BF01417860"},{"key":"2023041303292809000_28.1.217.26","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81224-6"},{"key":"2023041303292809000_28.1.217.27","doi-asserted-by":"crossref","first-page":"8070","DOI":"10.1523\/JNEUROSCI.23-22-08070.2003","article-title":"The biological clock nucleus: a multiphasic oscillator network regulated by light","volume":"23","author":"Quintero","year":"2003","journal-title":"J Neurosci"},{"key":"2023041303292809000_28.1.217.28","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065635"},{"key":"2023041303292809000_28.1.217.29","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81676-1"},{"key":"2023041303292809000_28.1.217.30","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1234-05.2006"},{"key":"2023041303292809000_28.1.217.31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2436298100"},{"key":"2023041303292809000_28.1.217.32","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5138-A-04.2005"},{"key":"2023041303292809000_28.1.217.33","doi-asserted-by":"crossref","unstructured":"Sheeba V Gu H Sharma VK O'Dowd DK Holmes TC (2008) Circadian- and light-dependent regulation of resting membrane potential and spontaneous action potential firing of Drosophila circadian pacemaker neurons. J Neurophysiol, in press.","DOI":"10.1152\/jn.00930.2007"},{"key":"2023041303292809000_28.1.217.34","doi-asserted-by":"publisher","DOI":"10.1038\/nature04192"},{"key":"2023041303292809000_28.1.217.35","doi-asserted-by":"publisher","DOI":"10.1126\/science.8178157"},{"key":"2023041303292809000_28.1.217.36","doi-asserted-by":"publisher","DOI":"10.1076\/brhm.30.2.149.1424"},{"key":"2023041303292809000_28.1.217.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.09.030"},{"key":"2023041303292809000_28.1.217.38","doi-asserted-by":"publisher","DOI":"10.1126\/science.1089287"},{"key":"2023041303292809000_28.1.217.39","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2538-05.2005"},{"key":"2023041303292809000_28.1.217.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2004.02.011"},{"key":"2023041303292809000_28.1.217.41","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03174"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.4087-07.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T18:26:10Z","timestamp":1681410370000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.4087-07.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1,2]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2008,1,2]]},"published-print":{"date-parts":[[2008,1,2]]}},"alternative-id":["10.1523\/JNEUROSCI.4087-07.2008"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.4087-07.2008","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1,2]]}}}