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AVP travels through the bloodstream to the kidney, where it promotes water conservation. Knockdown of HNS NKCC2 elicited profound effects on fluid balance following ingestion of a high-salt solution\u2014rats produced significantly more urine, concomitant with increases in fluid intake and plasma osmolality. Since NKCC2 is the molecular target of the loop diuretics bumetanide and furosemide, we asked about their effects on HNS function following disturbed water balance. Dehydration-evoked GABA-mediated excitation of AVP neurons was reversed by bumetanide, and furosemide blocked AVP release, both<jats:italic>in vivo<\/jats:italic>and in hypothalamic explants. Thus, NKCC2-dependent brain mechanisms that regulate osmotic stability are disrupted by loop diuretics in rats.<\/jats:p>","DOI":"10.1523\/jneurosci.4121-14.2015","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T16:52:02Z","timestamp":1427907122000},"page":"5144-5155","source":"Crossref","is-referenced-by-count":41,"title":["Osmoregulation Requires Brain Expression of the Renal Na-K-2Cl Cotransporter NKCC2"],"prefix":"10.1523","volume":"35","author":[{"given":"Agnieszka","family":"Konopacka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3337-4490","authenticated-orcid":false,"given":"Song T.","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael 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