{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T04:25:59Z","timestamp":1771993559155,"version":"3.50.1"},"reference-count":64,"publisher":"Society for Neuroscience","issue":"49","license":[{"start":{"date-parts":[[2011,6,8]],"date-time":"2011-06-08T00:00:00Z","timestamp":1307491200000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2010,12,8]]},"abstract":"<jats:p>Mechanisms influencing the development of olfactory bulb glomeruli are poorly understood. While odor receptors (ORs) play an important role in olfactory sensory neuron (OSN) axon targeting\/coalescence (Mombaerts et al., 1996; Wang et al., 1998; Feinstein and Mombaerts, 2004), recent work showed that G protein activation alone is sufficient to induce OSN axon coalescence (Imai et al., 2006; Chesler et al., 2007), suggesting an activity-dependent mechanism in glomerular development. Consistent with these data, OSN axon projections and convergence are perturbed in mice deficient for adenylyl cyclase III, which is downstream from the OR and catalyzes the conversion of ATP to cAMP. However, in cyclic nucleotide-gated (CNG) channel knock-out mice OSN axons are only transiently perturbed (Lin et al., 2000), suggesting that the CNG channel may not be the sole target of cAMP. This prompted us to investigate an alternative channel, the hyperpolarization-activated, cyclic nucleotide-gated cation channel (HCN), as a potential developmental target of cAMP in OSNs. Here, we demonstrate that HCN channels are developmentally precocious in OSNs and therefore are plausible candidates for affecting OSN axon development. Inhibition of HCN channels in dissociated OSNs significantly reduced neurite outgrowth. Moreover, in HCN1 knock-out mice the formation of glomeruli was delayed in parallel with perturbations of axon organization in the olfactory nerve. These data support the hypothesis that the outgrowth and coalescence of OSN axons is, at least in part, subject to activity-dependent mechanisms mediated via HCN channels.<\/jats:p>","DOI":"10.1523\/jneurosci.4225-10.2010","type":"journal-article","created":{"date-parts":[[2010,12,8]],"date-time":"2010-12-08T17:50:17Z","timestamp":1291830617000},"page":"16498-16508","source":"Crossref","is-referenced-by-count":31,"title":["Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation"],"prefix":"10.1523","volume":"30","author":[{"given":"Arie S.","family":"Mobley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra M.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo C.","family":"Araneda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia R.","family":"Maurer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles A.","family":"Greer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"393","published-online":{"date-parts":[[2010,12,8]]},"reference":[{"key":"2023041303474940000_30.49.16498.1","doi-asserted-by":"publisher","DOI":"10.1038\/72072"},{"key":"2023041303474940000_30.49.16498.2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4699-06.2007"},{"key":"2023041303474940000_30.49.16498.3","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21540"},{"key":"2023041303474940000_30.49.16498.4","doi-asserted-by":"crossref","first-page":"4560","DOI":"10.1523\/JNEUROSCI.18-12-04560.1998","article-title":"Odorant response properties of convergent olfactory receptor neurons","volume":"18","author":"Bozza","year":"1998","journal-title":"J Neurosci"},{"key":"2023041303474940000_30.49.16498.5","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhk021"},{"key":"2023041303474940000_30.49.16498.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80200-7"},{"key":"2023041303474940000_30.49.16498.7","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.117.5.491"},{"key":"2023041303474940000_30.49.16498.8","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01352.2007"},{"key":"2023041303474940000_30.49.16498.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609215104"},{"key":"2023041303474940000_30.49.16498.10","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2217-07.2007"},{"key":"2023041303474940000_30.49.16498.11","doi-asserted-by":"publisher","DOI":"10.1242\/dev.006346"},{"key":"2023041303474940000_30.49.16498.12","doi-asserted-by":"crossref","first-page":"4428","DOI":"10.1523\/JNEUROSCI.19-11-04428.1999","article-title":"Chemoattraction and chemorepulsion of olfactory bulb axons by different secreted semaphorins","volume":"19","author":"de Castro","year":"1999","journal-title":"J Neurosci"},{"key":"2023041303474940000_30.49.16498.13","doi-asserted-by":"publisher","DOI":"10.1152\/jn.90263.2008"},{"key":"2023041303474940000_30.49.16498.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2443-09.2009"},{"key":"2023041303474940000_30.49.16498.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.05.011"},{"key":"2023041303474940000_30.49.16498.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.05.013"},{"key":"2023041303474940000_30.49.16498.17","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.100.1.45"},{"key":"2023041303474940000_30.49.16498.18","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050488"},{"key":"2023041303474940000_30.49.16498.19","doi-asserted-by":"publisher","DOI":"10.1111\/j.0953-816X.2004.03331.x"},{"key":"2023041303474940000_30.49.16498.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.08.018"},{"key":"2023041303474940000_30.49.16498.21","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.02756.x"},{"key":"2023041303474940000_30.49.16498.22","doi-asserted-by":"publisher","DOI":"10.1126\/science.1131794"},{"key":"2023041303474940000_30.49.16498.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.05.022"},{"key":"2023041303474940000_30.49.16498.24","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjg063"},{"key":"2023041303474940000_30.49.16498.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.11.058"},{"key":"2023041303474940000_30.49.16498.26","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81139-3"},{"key":"2023041303474940000_30.49.16498.27","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10910"},{"key":"2023041303474940000_30.49.16498.28","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1991.0024"},{"key":"2023041303474940000_30.49.16498.29","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(93)90040-H"},{"key":"2023041303474940000_30.49.16498.30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813224106"},{"key":"2023041303474940000_30.49.16498.31","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00228-6"},{"key":"2023041303474940000_30.49.16498.32","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80414-6"},{"key":"2023041303474940000_30.49.16498.33","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81387-2"},{"key":"2023041303474940000_30.49.16498.34","doi-asserted-by":"publisher","DOI":"10.1515\/BC.1999.121"},{"key":"2023041303474940000_30.49.16498.35","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.02634.x"},{"key":"2023041303474940000_30.49.16498.36","doi-asserted-by":"publisher","DOI":"10.1002\/cne.11039"},{"key":"2023041303474940000_30.49.16498.37","doi-asserted-by":"crossref","first-page":"9713","DOI":"10.1523\/JNEUROSCI.21-24-09713.2001","article-title":"Structure and emergence of specific olfactory glomeruli in the mouse","volume":"21","author":"Potter","year":"2001","journal-title":"J Neurosci"},{"key":"2023041303474940000_30.49.16498.38","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.092101.142734"},{"key":"2023041303474940000_30.49.16498.39","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1776-10.2010"},{"key":"2023041303474940000_30.49.16498.40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708153105"},{"key":"2023041303474940000_30.49.16498.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.07.003"},{"key":"2023041303474940000_30.49.16498.42","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03304.x"},{"key":"2023041303474940000_30.49.16498.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.10.031"},{"key":"2023041303474940000_30.49.16498.44","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21595"},{"key":"2023041303474940000_30.49.16498.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.01.049"},{"key":"2023041303474940000_30.49.16498.46","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.04880.x"},{"key":"2023041303474940000_30.49.16498.47","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00421-5"},{"key":"2023041303474940000_30.49.16498.48","doi-asserted-by":"crossref","first-page":"2469","DOI":"10.1523\/JNEUROSCI.22-07-02469.2002","article-title":"Specificity of glomerular targeting by olfactory sensory axons","volume":"22","author":"Treloar","year":"2002","journal-title":"J Neurosci"},{"key":"2023041303474940000_30.49.16498.49","doi-asserted-by":"crossref","unstructured":"Treloar HB Miller AM Ray A Greer CA (2009) in The neurobiology of olfaction, Development of the olfactory system, ed Menini A (CRC, Boca Raton, FL), pp 131\u2013156.","DOI":"10.1201\/9781420071993-c5"},{"key":"2023041303474940000_30.49.16498.50","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1152\/jn.1999.81.1.149","article-title":"Dopamine modulates inwardly rectifying hyperpolarization-activated current (Ih) in cultured rat olfactory receptor neurons","volume":"81","author":"Vargas","year":"1999","journal-title":"J Neurophysiol"},{"key":"2023041303474940000_30.49.16498.51","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00841.2006"},{"key":"2023041303474940000_30.49.16498.52","doi-asserted-by":"publisher","DOI":"10.1038\/35081088"},{"key":"2023041303474940000_30.49.16498.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2006.01.013"},{"key":"2023041303474940000_30.49.16498.54","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81145-9"},{"key":"2023041303474940000_30.49.16498.55","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480270107"},{"key":"2023041303474940000_30.49.16498.56","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.007583"},{"key":"2023041303474940000_30.49.16498.57","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(04)00224-7"},{"key":"2023041303474940000_30.49.16498.58","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.19.030196.001315"},{"key":"2023041303474940000_30.49.16498.59","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00262-8"},{"key":"2023041303474940000_30.49.16498.60","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81140-X"},{"key":"2023041303474940000_30.49.16498.61","doi-asserted-by":"publisher","DOI":"10.1038\/47501"},{"key":"2023041303474940000_30.49.16498.62","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.11.012"},{"key":"2023041303474940000_30.49.16498.63","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0699-07.2007"},{"key":"2023041303474940000_30.49.16498.64","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2666"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.4225-10.2010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T20:12:53Z","timestamp":1681416773000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.4225-10.2010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,8]]},"references-count":64,"journal-issue":{"issue":"49","published-online":{"date-parts":[[2010,12,8]]},"published-print":{"date-parts":[[2010,12,8]]}},"alternative-id":["10.1523\/JNEUROSCI.4225-10.2010"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.4225-10.2010","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,8]]}}}