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Mammalian Na<jats:sup>+<\/jats:sup>channels are composed of one pore-forming \u03b1-subunit and two \u03b2-subunits.<jats:italic>SCN1B<\/jats:italic>encodes the Na<jats:sup>+<\/jats:sup>channel \u03b21-subunit that modulates channel gating and voltage dependence, regulates channel cell surface expression, and functions as a cell adhesion molecule (CAM). We recently identified<jats:italic>scn1ba<\/jats:italic>, a zebrafish ortholog of<jats:italic>SCN1B<\/jats:italic>. Here we report that zebrafish express a second \u03b21-like paralog,<jats:italic>scn1bb<\/jats:italic>. In contrast to the restricted expression of<jats:italic>scn1ba<\/jats:italic>mRNA in excitable cells, we detected<jats:italic>scn1bb<\/jats:italic>transcripts and protein in several ectodermal derivatives including neurons, glia, the lateral line, peripheral sensory structures, and tissues derived from other germ layers such as the pronephros. As expected for \u03b21-subunits, elimination of Scn1bb protein<jats:italic>in vivo<\/jats:italic>by morpholino knock-down reduced Na<jats:sup>+<\/jats:sup>current amplitudes in Rohon-Beard neurons of zebrafish embryos, consistent with effects observed in heterologous systems. Further, after Scn1bb knock-down, zebrafish embryos displayed defects in Rohon-Beard mediated touch sensitivity, demonstrating the significance of Scn1bb modulation of Na<jats:sup>+<\/jats:sup>current to organismal behavior. In addition to effects associated with Na<jats:sup>+<\/jats:sup>current modulation, Scn1bb knockdown produced phenotypes consistent with CAM functions. In particular, morpholino knock-down led to abnormal development of ventrally projecting spinal neuron axons, defasciculation of the olfactory nerve, and increased hair cell number in the inner ear. We propose that, in addition to modulation of electrical excitability, Scn1bb plays critical developmental roles by functioning as a CAM in the zebrafish embryonic nervous system.<\/jats:p>","DOI":"10.1523\/jneurosci.4329-08.2008","type":"journal-article","created":{"date-parts":[[2008,11,19]],"date-time":"2008-11-19T17:32:41Z","timestamp":1227115961000},"page":"12510-12522","source":"Crossref","is-referenced-by-count":21,"title":["<i>scn1bb<\/i>, a Zebrafish Ortholog of<i>SCN1B<\/i>Expressed in Excitable and Nonexcitable Cells, Affects Motor Neuron Axon Morphology and Touch Sensitivity"],"prefix":"10.1523","volume":"28","author":[{"given":"Amanda J.","family":"Fein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa A.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily A.","family":"Slat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeles B.","family":"Ribera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori L.","family":"Isom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2008,11,19]]},"reference":[{"key":"2023041303350885000_28.47.12510.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81126-5"},{"key":"2023041303350885000_28.47.12510.2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.1172"},{"key":"2023041303350885000_28.47.12510.3","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.12.9.1191"},{"key":"2023041303350885000_28.47.12510.4","doi-asserted-by":"crossref","unstructured":"Brackenbury WJ Djamgoz MB Isom LL (2008a) An emerging role for voltage-gated Na+ channels in cellular migration: regulation of central nervous system development and potentiation of invasive cancers. 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