{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T03:08:35Z","timestamp":1780542515094,"version":"3.54.1"},"reference-count":48,"publisher":"Society for Neuroscience","issue":"16","license":[{"start":{"date-parts":[[2014,10,16]],"date-time":"2014-10-16T00:00:00Z","timestamp":1413417600000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2014,4,16]]},"abstract":"<jats:p>Myocilin is a secreted glycoprotein that belongs to a family of olfactomedin domain-containing proteins. Although myocilin is detected in several ocular and nonocular tissues, the only reported human pathology related to mutations in the<jats:italic>MYOCILIN<\/jats:italic>gene is primary open-angle glaucoma. Functions of myocilin are poorly understood. Here we demonstrate that myocilin is a mediator of oligodendrocyte differentiation and is involved in the myelination of the optic nerve in mice. Myocilin is expressed and secreted by optic nerve astrocytes. Differentiation of optic nerve oligodendrocytes is delayed in<jats:italic>Myocilin<\/jats:italic>-null mice. Optic nerves of<jats:italic>Myocilin<\/jats:italic>-null mice contain reduced levels of several myelin-associated proteins including myelin basic protein, myelin proteolipid protein, and 2\u20323\u2032-cyclic nucleotide 3\u2032-phosphodiesterase compared with those of wild-type littermates. This leads to reduced myelin sheath thickness of optic nerve axons in<jats:italic>Myocilin<\/jats:italic>-null mice compared with wild-type littermates, and this difference is more pronounced at early postnatal stages compared with adult mice. Myocilin also affects differentiation of oligodendrocyte precursors<jats:italic>in vitro<\/jats:italic>. Its addition to primary cultures of differentiating oligodendrocyte precursors increases levels of tested markers of oligodendrocyte differentiation and stimulates elongation of oligodendrocyte processes. Myocilin stimulation of oligodendrocyte differentiation occurs through the NgR1\/Lingo-1 receptor complex. Myocilin physically interacts with Lingo-1 and may be considered as a Lingo-1 ligand. Myocilin-induced elongation of oligodendrocyte processes may be mediated by activation of FYN and suppression of RhoA GTPase.<\/jats:p>","DOI":"10.1523\/jneurosci.4731-13.2014","type":"journal-article","created":{"date-parts":[[2014,4,16]],"date-time":"2014-04-16T16:38:01Z","timestamp":1397666281000},"page":"5539-5551","source":"Crossref","is-referenced-by-count":28,"title":["Myocilin Is Involved in NgR1\/Lingo-1-Mediated Oligodendrocyte Differentiation and Myelination of the Optic Nerve"],"prefix":"10.1523","volume":"34","author":[{"given":"Heung Sun","family":"Kwon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Nakaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mones","family":"Abu-Asab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Sug","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislav I.","family":"Tomarev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2014,4,16]]},"reference":[{"key":"2023041304102421000_34.16.5539.1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.12.2091"},{"key":"2023041304102421000_34.16.5539.2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22378"},{"key":"2023041304102421000_34.16.5539.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.10.035"},{"key":"2023041304102421000_34.16.5539.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.06.028"},{"key":"2023041304102421000_34.16.5539.5","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4178-07.2008"},{"key":"2023041304102421000_34.16.5539.6","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0663fje"},{"key":"2023041304102421000_34.16.5539.7","doi-asserted-by":"publisher","DOI":"10.1126\/science.1190927"},{"key":"2023041304102421000_34.16.5539.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.06.026"},{"key":"2023041304102421000_34.16.5539.9","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20688"},{"key":"2023041304102421000_34.16.5539.10","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1093\/jn\/131.9.2378","article-title":"Magnesium deficiency differentially affects the retina and visual cortex of intact rats","volume":"131","author":"Goto","year":"2001","journal-title":"J Nutr"},{"key":"2023041304102421000_34.16.5539.11","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1242\/dev.111.4.1061","article-title":"Proliferation and differentiation potential of rat forebrain oligodendroglial progenitors both in vitro and in vivo","volume":"111","author":"Hardy","year":"1991","journal-title":"Development"},{"key":"2023041304102421000_34.16.5539.12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.366179"},{"key":"2023041304102421000_34.16.5539.13","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090853"},{"key":"2023041304102421000_34.16.5539.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.10.162"},{"key":"2023041304102421000_34.16.5539.15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.224063"},{"key":"2023041304102421000_34.16.5539.16","first-page":"729","article-title":"Localization of myocilin\/trabecular meshwork\u2013inducible glucocorticoid response protein in the human eye","volume":"41","author":"Karali","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2023041304102421000_34.16.5539.17","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.22.7707-7713.2001"},{"key":"2023041304102421000_34.16.5539.18","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22701"},{"key":"2023041304102421000_34.16.5539.19","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01274-08"},{"key":"2023041304102421000_34.16.5539.20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.446138"},{"key":"2023041304102421000_34.16.5539.21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.422972"},{"key":"2023041304102421000_34.16.5539.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.06.006"},{"key":"2023041304102421000_34.16.5539.23","first-page":"517","article-title":"Localization of the stress proteins alpha B-crystallin and trabecular meshwork inducible glucocorticoid response protein in normal and glaucomatous trabecular meshwork","volume":"39","author":"L\u00fctjen-Drecoll","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2023041304102421000_34.16.5539.24","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0220"},{"key":"2023041304102421000_34.16.5539.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.07.007"},{"key":"2023041304102421000_34.16.5539.26","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3386"},{"key":"2023041304102421000_34.16.5539.27","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80942-3"},{"key":"2023041304102421000_34.16.5539.28","doi-asserted-by":"publisher","DOI":"10.1038\/nn1188"},{"key":"2023041304102421000_34.16.5539.29","doi-asserted-by":"publisher","DOI":"10.1038\/nn1460"},{"key":"2023041304102421000_34.16.5539.30","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095862"},{"key":"2023041304102421000_34.16.5539.31","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.389916"},{"key":"2023041304102421000_34.16.5539.32","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3591"},{"key":"2023041304102421000_34.16.5539.33","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10233"},{"key":"2023041304102421000_34.16.5539.34","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00934-4"},{"key":"2023041304102421000_34.16.5539.35","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt246"},{"key":"2023041304102421000_34.16.5539.36","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.5.978"},{"key":"2023041304102421000_34.16.5539.37","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0727-08.2008"},{"key":"2023041304102421000_34.16.5539.38","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(93)90213-K"},{"key":"2023041304102421000_34.16.5539.39","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200305018"},{"key":"2023041304102421000_34.16.5539.40","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.3.578"},{"key":"2023041304102421000_34.16.5539.41","doi-asserted-by":"publisher","DOI":"10.1006\/exer.2001.1063"},{"key":"2023041304102421000_34.16.5539.42","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5300.668"},{"key":"2023041304102421000_34.16.5539.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.08.017"},{"key":"2023041304102421000_34.16.5539.44","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2010.37"},{"key":"2023041304102421000_34.16.5539.45","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-1099"},{"key":"2023041304102421000_34.16.5539.46","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-7434"},{"key":"2023041304102421000_34.16.5539.47","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808751200"},{"key":"2023041304102421000_34.16.5539.48","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58183"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.4731-13.2014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T22:05:24Z","timestamp":1681423524000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.4731-13.2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,16]]},"references-count":48,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2014,4,16]]},"published-print":{"date-parts":[[2014,4,16]]}},"alternative-id":["10.1523\/JNEUROSCI.4731-13.2014"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.4731-13.2014","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,16]]}}}