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To understand the mechanism of PLS3 in SMA, we have analyzed<jats:italic>pls3<\/jats:italic>RNA and protein in zebrafish<jats:italic>smn<\/jats:italic>mutants. We show that Pls3 protein levels are severely decreased in<jats:italic>smn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>mutants without a reduction in<jats:italic>pls3<\/jats:italic>mRNA levels. Moreover, we show that both<jats:italic>pls3<\/jats:italic>mRNA and protein stability are unaffected when Smn is reduced. This indicates that SMN affects PLS3 protein production. We had previously shown that, in<jats:italic>smn<\/jats:italic>mutants, the presynaptic protein SV2 is decreased at neuromuscular junctions. Transgenically driving human PLS3 in motoneurons rescues the decrease in SV2 expression. To determine whether PLS3 could also rescue function, we performed behavioral analysis on<jats:italic>smn<\/jats:italic>mutants and found that they had a significant decrease in spontaneous swimming and turning. Driving PLS3 transgenically in motoneurons rescued both of these defects. These data show that PLS3 protein levels are dependent on SMN and that PLS3 is able to rescue the neuromuscular defects and corresponding movement phenotypes caused by low levels of Smn suggesting that decreased PLS3 contributes to SMA motor phenotypes.<\/jats:p>","DOI":"10.1523\/jneurosci.5808-11.2012","type":"journal-article","created":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T16:29:13Z","timestamp":1334161753000},"page":"5074-5084","source":"Crossref","is-referenced-by-count":65,"title":["Survival Motor Neuron Affects Plastin 3 Protein Levels Leading to Motor Defects"],"prefix":"10.1523","volume":"32","author":[{"given":"Le 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