{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:42:58Z","timestamp":1791261778422,"version":"4.1.0"},"reference-count":78,"publisher":"Society for Neuroscience","issue":"38","license":[{"start":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T00:00:00Z","timestamp":1332288000000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2011,9,21]]},"abstract":"<jats:p>The mechanisms governing the recruitment of functional glutamate receptors at nascent excitatory postsynapses following initial axon-dendrite contact remain unclear. We examined here the ability of neurexin\/neuroligin adhesions to mobilize AMPA-type glutamate receptors (AMPARs) at postsynapses through a diffusion\/trap process involving the scaffold molecule PSD-95. Using single nanoparticle tracking in primary rat and mouse hippocampal neurons overexpressing or lacking neuroligin-1 (Nlg1), a striking inverse correlation was found between AMPAR diffusion and Nlg1 expression level. The use of Nlg1 mutants and inhibitory RNAs against PSD-95 demonstrated that this effect depended on intact Nlg1\/PSD-95 interactions. Furthermore, functional AMPARs were recruited within 1 h at nascent Nlg1\/PSD-95 clusters assembled by neurexin-1\u03b2 multimers, a process requiring AMPAR membrane diffusion. Triggering novel neurexin\/neuroligin adhesions also caused a depletion of PSD-95 from native synapses and a drop in AMPAR miniature EPSCs, indicating a competitive mechanism. Finally, both AMPAR level at synapses and AMPAR-dependent synaptic transmission were diminished in hippocampal slices from newborn Nlg1 knock-out mice, confirming an important role of Nlg1 in driving AMPARs to nascent synapses. Together, these data reveal a mechanism by which membrane-diffusing AMPARs can be rapidly trapped at PSD-95 scaffolds assembled at nascent neurexin\/neuroligin adhesions, in competition with existing synapses.<\/jats:p>","DOI":"10.1523\/jneurosci.6439-10.2011","type":"journal-article","created":{"date-parts":[[2011,9,21]],"date-time":"2011-09-21T16:50:18Z","timestamp":1316623818000},"page":"13500-13515","source":"Crossref","is-referenced-by-count":159,"title":["Neurexin-Neuroligin Adhesions Capture Surface-Diffusing AMPA Receptors through PSD-95 Scaffolds"],"prefix":"10.1523","volume":"31","author":[{"given":"Magali","family":"Mondin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Labrousse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Hosy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Heine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Tessier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Levet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Poujol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Blanchet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Choquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Thoumine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2011,9,21]]},"reference":[{"key":"2023041303523721000_31.38.13500.1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1235-06.2006"},{"key":"2023041303523721000_31.38.13500.2","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8104-4-17"},{"key":"2023041303523721000_31.38.13500.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.01.030"},{"key":"2023041303523721000_31.38.13500.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711669105"},{"key":"2023041303523721000_31.38.13500.5","doi-asserted-by":"publisher","DOI":"10.1038\/nature00780"},{"key":"2023041303523721000_31.38.13500.6","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2001.1012"},{"key":"2023041303523721000_31.38.13500.7","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3819-03.2004"},{"key":"2023041303523721000_31.38.13500.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.02.014"},{"key":"2023041303523721000_31.38.13500.9","doi-asserted-by":"publisher","DOI":"10.1038\/35050030"},{"key":"2023041303523721000_31.38.13500.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.08.016"},{"key":"2023041303523721000_31.38.13500.11","doi-asserted-by":"publisher","DOI":"10.1126\/science.1107470"},{"key":"2023041303523721000_31.38.13500.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.05.029"},{"key":"2023041303523721000_31.38.13500.13","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1152\/jn.2000.84.3.1573","article-title":"Distribution, density, and clustering of functional glutamate receptors before and after synaptogenesis in hippocampal neurons","volume":"84","author":"Cottrell","year":"2000","journal-title":"J Neurophysiol"},{"key":"2023041303523721000_31.38.13500.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2007.01.011"},{"key":"2023041303523721000_31.38.13500.15","doi-asserted-by":"publisher","DOI":"10.1038\/nn1074"},{"key":"2023041303523721000_31.38.13500.16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.3269"},{"key":"2023041303523721000_31.38.13500.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.12.019"},{"key":"2023041303523721000_31.38.13500.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.010"},{"key":"2023041303523721000_31.38.13500.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.09.012"},{"key":"2023041303523721000_31.38.13500.20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811025106"},{"key":"2023041303523721000_31.38.13500.21","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00009-X"},{"key":"2023041303523721000_31.38.13500.22","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2338"},{"key":"2023041303523721000_31.38.13500.23","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(02)02152-5"},{"key":"2023041303523721000_31.38.13500.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.01.015"},{"key":"2023041303523721000_31.38.13500.25","doi-asserted-by":"crossref","unstructured":"Goslin K Asmussen H Banker G (1991) in Culturing nerve cells, Rat hippocampal neurons in low-density culture, eds Banker G Goslin K (MIT, Cambridge, MA), Ed 2, pp 339\u2013370.","DOI":"10.7551\/mitpress\/4913.003.0020"},{"key":"2023041303523721000_31.38.13500.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.11.035"},{"key":"2023041303523721000_31.38.13500.27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040370"},{"key":"2023041303523721000_31.38.13500.28","doi-asserted-by":"crossref","first-page":"5552","DOI":"10.1523\/JNEUROSCI.22-13-05552.2002","article-title":"Spontaneous unitary synaptic activity in CA1 pyramidal neurons during early postnatal development: constant contribution of AMPA and NMDA receptors","volume":"22","author":"Groc","year":"2002","journal-title":"J Neurosci"},{"key":"2023041303523721000_31.38.13500.29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605238103"},{"key":"2023041303523721000_31.38.13500.30","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3349-07.2007"},{"key":"2023041303523721000_31.38.13500.31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2009.07007.x"},{"key":"2023041303523721000_31.38.13500.32","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152089"},{"key":"2023041303523721000_31.38.13500.33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804007106"},{"key":"2023041303523721000_31.38.13500.34","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1206"},{"key":"2023041303523721000_31.38.13500.35","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(99)00095-3"},{"key":"2023041303523721000_31.38.13500.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.08.006"},{"key":"2023041303523721000_31.38.13500.37","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5331.1511"},{"key":"2023041303523721000_31.38.13500.38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001084107"},{"key":"2023041303523721000_31.38.13500.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.042"},{"key":"2023041303523721000_31.38.13500.40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3909-09.2010"},{"key":"2023041303523721000_31.38.13500.41","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.249"},{"key":"2023041303523721000_31.38.13500.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2008.02.014"},{"key":"2023041303523721000_31.38.13500.43","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0522-06.2006"},{"key":"2023041303523721000_31.38.13500.44","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.31.19518"},{"key":"2023041303523721000_31.38.13500.45","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413812200"},{"key":"2023041303523721000_31.38.13500.46","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.289"},{"key":"2023041303523721000_31.38.13500.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.01.017"},{"key":"2023041303523721000_31.38.13500.48","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.02.027"},{"key":"2023041303523721000_31.38.13500.49","doi-asserted-by":"publisher","DOI":"10.1038\/nature01755"},{"key":"2023041303523721000_31.38.13500.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.11.009"},{"key":"2023041303523721000_31.38.13500.51","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.02.036"},{"key":"2023041303523721000_31.38.13500.52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502038102"},{"key":"2023041303523721000_31.38.13500.53","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200805132"},{"key":"2023041303523721000_31.38.13500.54","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.06.007"},{"key":"2023041303523721000_31.38.13500.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.08.023"},{"key":"2023041303523721000_31.38.13500.56","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.012"},{"key":"2023041303523721000_31.38.13500.57","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.04.044"},{"key":"2023041303523721000_31.38.13500.58","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1523\/JNEUROSCI.20-03-01260.2000","article-title":"A developmental change in NMDA receptor-associated proteins at hippocampal synapses","volume":"20","author":"Sans","year":"2000","journal-title":"J Neurosci"},{"key":"2023041303523721000_31.38.13500.59","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80877-6"},{"key":"2023041303523721000_31.38.13500.60","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.05.016"},{"key":"2023041303523721000_31.38.13500.61","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2006.01.010"},{"key":"2023041303523721000_31.38.13500.62","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00321-X"},{"key":"2023041303523721000_31.38.13500.63","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.05.016"},{"key":"2023041303523721000_31.38.13500.64","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2825"},{"key":"2023041303523721000_31.38.13500.65","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0470-10.2010"},{"key":"2023041303523721000_31.38.13500.66","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.10.014"},{"key":"2023041303523721000_31.38.13500.67","doi-asserted-by":"publisher","DOI":"10.1038\/nature07456"},{"key":"2023041303523721000_31.38.13500.68","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth783"},{"key":"2023041303523721000_31.38.13500.69","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4256-04.2005"},{"key":"2023041303523721000_31.38.13500.70","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg463"},{"key":"2023041303523721000_31.38.13500.71","doi-asserted-by":"publisher","DOI":"10.1021\/cr078204m"},{"key":"2023041303523721000_31.38.13500.72","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.09.025"},{"key":"2023041303523721000_31.38.13500.73","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090262"},{"key":"2023041303523721000_31.38.13500.74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040271"},{"key":"2023041303523721000_31.38.13500.75","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.09.003"},{"key":"2023041303523721000_31.38.13500.76","doi-asserted-by":"publisher","DOI":"10.1038\/nn883"},{"key":"2023041303523721000_31.38.13500.77","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1523\/JNEUROSCI.16-06-01982.1996","article-title":"Evidence for multiple AMPA receptor complexes in hippocampal CA1\/CA2 neurons","volume":"16","author":"Wenthold","year":"1996","journal-title":"J Neurosci"},{"key":"2023041303523721000_31.38.13500.78","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0091-679X(04)75007-4","article-title":"Labeling cellular targets with semiconductor quantum dot conjugates","volume":"75","author":"Wu","year":"2004","journal-title":"Methods Cell Biol"}],"container-title":["The Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1523\/JNEUROSCI.6439-10.2011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T20:40:39Z","timestamp":1681418439000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jneurosci.org\/lookup\/doi\/10.1523\/JNEUROSCI.6439-10.2011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,21]]},"references-count":78,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2011,9,21]]},"published-print":{"date-parts":[[2011,9,21]]}},"alternative-id":["10.1523\/JNEUROSCI.6439-10.2011"],"URL":"https:\/\/doi.org\/10.1523\/jneurosci.6439-10.2011","relation":{},"ISSN":["0270-6474","1529-2401"],"issn-type":[{"value":"0270-6474","type":"print"},{"value":"1529-2401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,21]]}}}