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The BgtRs mediate most of the response elicited by nicotine, because the BgtR-specific antagonists methyllycaconitine and \u03b1-Bgt block \u223c90% of the whole-cell current. The binding of nicotinic agonists to cell-surface BgtRs was highly cooperative with four different agonists showing Hill coefficients in the range of 2.3\u20132.4. A similar agonist binding cooperativity was observed for BgtR homomers formed from chimeric \u03b17\/5HT3 subunits expressed in tsA 201 cells. Two classes of agonist binding sites, in the ratio of 4:1 for PC12 cell BgtRs and 3:1 for \u03b17\/5HT3 BgtRs, were revealed by bromoacetylcholine alkylation of the reduced sites on both PC12 BgtRs and \u03b17\/5HT3 BgtRs. We conclude from this data that PC12 BgtRs and \u03b17\/5HT3 homomers contain at least three distinguishable agonist binding sites and thus are different from other nicotinic receptors.<\/jats:p>","DOI":"10.1523\/jneurosci.17-21-08201.1997","type":"journal-article","created":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T00:21:06Z","timestamp":1522714866000},"page":"8201-8212","source":"Crossref","is-referenced-by-count":75,"title":["Neuronal \u03b1-Bungarotoxin Receptors Differ Structurally from Other Nicotinic Acetylcholine Receptors"],"prefix":"10.1523","volume":"17","author":[{"given":"Fatima","family":"Rangwala","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renaldo C.","family":"Drisdel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Rakhilin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Ko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pramod","family":"Atluri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy B.","family":"Harkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron P.","family":"Fox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suleiman B.","family":"Salman, and William N. 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