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SARS\u2010CoV\u20102 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate\n                    <jats:italic>ACE2<\/jats:italic>\n                    and\n                    <jats:italic>TMPRSS2<\/jats:italic>\n                    expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While\n                    <jats:italic>TMPRSS2<\/jats:italic>\n                    is strongly expressed in both tissues, in the subsegmental bronchial branches\n                    <jats:italic>ACE2<\/jats:italic>\n                    is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS\u2010CoV\u20102 infection. Our data provide a rich resource for future investigations of COVID\u201019 infection and pathogenesis.\n                  <\/jats:p>","DOI":"10.15252\/embj.20105114","type":"journal-article","created":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T13:12:26Z","timestamp":1587129146000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1157,"title":["SARS\u2010CoV\u20102 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells"],"prefix":"10.1038","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7045-6327","authenticated-orcid":false,"given":"Soeren","family":"Lukassen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Lorenz","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Trefzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas C","family":"Kahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc A","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Muley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hauke","family":"Winter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Meister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Veith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes W","family":"Boots","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca P","family":"Hennig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4402-2159","authenticated-orcid":false,"given":"Michael","family":"Kreuter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7036-342X","authenticated-orcid":false,"given":"Christian","family":"Conrad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0034-4036","authenticated-orcid":false,"given":"Roland","family":"Eils","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,14]]},"reference":[{"key":"BFEMBJ20105114_CR1","doi-asserted-by":"crossref","first-page":"e0209648","DOI":"10.1371\/journal.pone.0209648","volume":"13","author":"TE Bakken","year":"2018","unstructured":"Bakken TE, Hodge RD, Miller JA, Yao Z, Nguyen TN, Aevermann B, Barkan E, Bertagnolli D, Casper T, Dee N et\u00a0al (2018) Single\u2010nucleus and single\u2010cell transcriptomes compared in matched cortical cell types. 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