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Similarly, many B\u2010cell lymphomas, including Burkitt lymphoma (BL), require continuous BCR signaling for their tumor growth. This growth is driven by immunoreceptor tyrosine\u2010based activation motif (ITAM) and PI3 kinase (PI3K) signaling. Here, we employ CRISPR\/Cas9 to delete BCR and B\u2010cell co\u2010receptor genes in the human BL cell line Ramos. We find that Ramos B cells require the expression of the BCR signaling component Ig\u03b2 (CD79b), and the co\u2010receptor CD19, for their fitness and competitive growth in culture. Furthermore, we show that in the absence of any other BCR component, Ig\u03b2 can be expressed on the B\u2010cell surface, where it is found in close proximity to CD19 and signals in an ITAM\u2010dependent manner. These data suggest that Ig\u03b2 and CD19 are part of an alternative B\u2010cell signaling module that use continuous ITAM\/PI3K signaling to promote the survival of B lymphoma and normal B cells.<\/jats:p>","DOI":"10.15252\/embj.201797980","type":"journal-article","created":{"date-parts":[[2018,4,18]],"date-time":"2018-04-18T08:50:28Z","timestamp":1524041428000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells"],"prefix":"10.1038","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0106-057X","authenticated-orcid":false,"given":"Xiaocui","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5969-2553","authenticated-orcid":false,"given":"Kathrin","family":"Kl\u00e4sener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseena M","family":"Iype","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1751-1056","authenticated-orcid":false,"given":"Martin","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Palash C","family":"Maity","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Cavallari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8197-5413","authenticated-orcid":false,"given":"Jianying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-7198","authenticated-orcid":false,"given":"Michael","family":"Reth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,4,18]]},"reference":[{"key":"BFEMBJ201797980_CR1","doi-asserted-by":"crossref","first-page":"5231","DOI":"10.1073\/pnas.1621512114","volume":"114","author":"M Becker","year":"2017","unstructured":"Becker M, Hobeika E, Jumaa H, Reth M, Maity PC (2017) CXCR4 signaling and function require the expression of the IgD\u2010class B\u2010cell antigen receptor. 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