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Plant and invertebrate cells utilise mostly RNA interference (RNAi), an RNA\u2010based mechanism, for cell\u2010intrinsic immunity to viruses while vertebrates rely on the protein\u2010based interferon (IFN)\u2010driven innate immune system for the same purpose. The RNAi machinery is conserved in vertebrate cells, yet whether antiviral RNAi is still active in mammals and functionally relevant to mammalian antiviral defence is intensely debated. 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