{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:25:40Z","timestamp":1790735140322,"version":"4.1.0"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"21","license":[{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["ALTF 1487\u20102015"],"award-info":[{"award-number":["ALTF 1487\u20102015"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"Department of Health|National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1124612"],"award-info":[{"award-number":["GNT1124612"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation|Medical Research Council","doi-asserted-by":"publisher","award":["U105181010"],"award-info":[{"award-number":["U105181010"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO J"],"published-print":{"date-parts":[[2019,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inflammasomes are potent innate immune signalling complexes that couple cytokine release with pro\u2010inflammatory cell death. However, pathogens have evolved strategies to evade this cell autonomous system. Here, we show how antibodies combine with innate sensors in primary human macrophages to detect viral infection and activate the inflammasome. Our data demonstrate that antibody opsonisation of virions can activate macrophages in multiple ways. In the first, antibody binding of adenovirus causes lysosomal damage, activating NLRP3 to drive inflammasome formation and IL\u20101\u03b2 release. Importantly, this mechanism enhances virion capture but not infection and is accompanied by cell death, denying the opportunity for viral replication. Unexpectedly, we also find that antibody\u2010coated viruses, which successfully escape into the cytosol, trigger a second system of inflammasome activation. These viruses are intercepted by the cytosolic antibody receptor TRIM21 and the DNA sensor cGAS. Together, these sensors stimulate both NLRP3 inflammasome formation and NF\u03baB activation, driving dose\u2010dependent IL\u20101\u03b2 and TNF secretion, without inducing cell death. Our data highlight the importance of cooperativity between multiple sensing networks to expose viruses to the inflammasome pathway, which is particularly important for how our innate immune system responds to infection in the presence of pre\u2010existing immunity.<\/jats:p>","DOI":"10.15252\/embj.2018101365","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T02:32:00Z","timestamp":1567132320000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Antibody and DNA sensing pathways converge to activate the inflammasome during primary human macrophage infection"],"prefix":"10.1038","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9098-4493","authenticated-orcid":false,"given":"Larisa I","family":"Labzin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Bottermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Rodriguez\u2010Silvestre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stian","family":"Foss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Terje","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Vaysburd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean","family":"Clift","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2131-0334","authenticated-orcid":false,"given":"Leo C","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,8,29]]},"reference":[{"key":"BFEMBJ2018101365_CR1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.virol.2011.01.019","volume":"412","author":"AU Barlan","year":"2011","unstructured":"Barlan AU, Danthi P, Wiethoff CM (2011a) Lysosomal localization and mechanism of membrane penetration influence nonenveloped virus activation of the NLRP3 inflammasome. Virology 412: 306\u2013314","journal-title":"Virology"},{"key":"BFEMBJ2018101365_CR2","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1128\/JVI.01265-10","volume":"85","author":"AU Barlan","year":"2011","unstructured":"Barlan AU, Griffin TM, Mcguire KA, Wiethoff CM (2011b) Adenovirus membrane penetration activates the NLRP3 inflammasome. J Virol 85: 146\u2013155","journal-title":"J Virol"},{"key":"BFEMBJ2018101365_CR3","doi-asserted-by":"crossref","first-page":"37457","DOI":"10.1038\/srep37457","volume":"6","author":"M Bottermann","year":"2016","unstructured":"Bottermann M, Lode HE, Watkinson RE, Foss S, Sandlie I, Andersen JT, James LC (2016) Antibody\u2010antigen kinetics constrain intracellular humoral immunity. Sci Rep 6: 37457","journal-title":"Sci Rep"},{"key":"BFEMBJ2018101365_CR4","doi-asserted-by":"crossref","first-page":"10440","DOI":"10.1073\/pnas.1806314115","volume":"115","author":"M Bottermann","year":"2018","unstructured":"Bottermann M, Foss S, van Tienen LM, Vaysburd M, Cruickshank J, O'Connell K, Clark J, Mayes K, Higginson K, Hirst JC et\u00a0al (2018) TRIM21 mediates antibody inhibition of adenovirus\u2010based gene delivery and vaccination. Proc Natl Acad Sci 115: 10440\u201310445","journal-title":"Proc Natl Acad Sci"},{"key":"BFEMBJ2018101365_CR5","first-page":"e02106","volume":"91","author":"LA Castro\u2010Jorge","year":"2017","unstructured":"Castro\u2010Jorge LA, Pretto CD, Smith AB, Foreman O, Carnahan KE, Spindler KR (2017) A protective role for interleukin\u20101 signaling during mouse adenovirus type 1\u2010induced encephalitis. J Virol 91: e02106\u2013e02116","journal-title":"J Virol"},{"key":"BFEMBJ2018101365_CR6","doi-asserted-by":"crossref","first-page":"e51057","DOI":"10.1371\/journal.pone.0051057","volume":"7","author":"RWY Chan","year":"2012","unstructured":"Chan RWY, Leung CYH, Nicholls JM, Peiris JSM, Chan MCW (2012) Proinflammatory cytokine response and viral replication in mouse bone marrow derived macrophages infected with influenza H1N1 and H5N1 viruses. PLoS ONE 7: e51057","journal-title":"PLoS ONE"},{"key":"BFEMBJ2018101365_CR7","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.mib.2013.02.004","volume":"16","author":"KW Chen","year":"2013","unstructured":"Chen KW, Schroder K (2013) Antimicrobial functions of inflammasomes. Curr Opin Microbiol 16: 311\u2013318","journal-title":"Curr Opin Microbiol"},{"key":"BFEMBJ2018101365_CR8","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.celrep.2014.06.028","volume":"8","author":"KW Chen","year":"2014","unstructured":"Chen KW, Gro\u00df CJ, Sotomayor FV, Stacey KJ, Tschopp J, Sweet MJ, Schroder K (2014) The neutrophil NLRC4 inflammasome selectively promotes IL\u20101\u03b2 maturation without pyroptosis during acute salmonella challenge. Cell Rep 8: 570\u2013582","journal-title":"Cell Rep"},{"key":"BFEMBJ2018101365_CR9","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.tim.2014.09.007","volume":"23","author":"I\u2010Y Chen","year":"2015","unstructured":"Chen I\u2010Y, Ichinohe T (2015) Response of host inflammasomes to viral infection. Trends Microbiol 23: 55\u201363","journal-title":"Trends Microbiol"},{"key":"BFEMBJ2018101365_CR10","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1016\/j.cell.2017.10.033","volume":"171","author":"D Clift","year":"2017","unstructured":"Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M (2017) A method for the acute and rapid degradation of endogenous proteins. Cell 171: 1692\u20131706.e18","journal-title":"Cell"},{"key":"BFEMBJ2018101365_CR11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nm.3806","volume":"21","author":"RC Coll","year":"2015","unstructured":"Coll RC, Robertson AAB, Chae JJ, Higgins SC, Mu\u00f1oz\u2010Planillo R, Inserra MC, Vetter I, Dungan LS, Monks BG, Stutz A et\u00a0al (2015) A small\u2010molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases. Nat Med 21: 248\u2013255","journal-title":"Nat Med"},{"key":"BFEMBJ2018101365_CR12","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1126\/science.1156995","volume":"320","author":"C Dostert","year":"2008","unstructured":"Dostert C, Petrilli V, Van Bruggen R, Steele C, Mossman BT, Tschopp J (2008) Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science 320: 674\u2013677","journal-title":"Science"},{"key":"BFEMBJ2018101365_CR13","first-page":"19","volume-title":"The Inflammasome. Methods in molecular biology (methods and protocols)","author":"P Duewell","year":"2013","unstructured":"Duewell P, Latz E (2013) Assessment and quantification of crystal\u2010induced lysosomal damage. In The Inflammasome. Methods in molecular biology (methods and protocols), De Nardo CM, Latz E (eds), Vol. 1040, pp. 19\u201327. Totowa, NJ: Humana Press"},{"key":"BFEMBJ2018101365_CR14","doi-asserted-by":"crossref","first-page":"e1005871","DOI":"10.1371\/journal.ppat.1005871","volume":"12","author":"K Eichholz","year":"2016","unstructured":"Eichholz K, Bru T, Tran TTP, Fernandes P, Welles H, Mennechet FJD, Manel N, Alves P, Perreau M, Kremer EJ (2016) Immune\u2010complexed adenovirus induce AIM2\u2010mediated pyroptosis in human dendritic cells. PLoS Pathog 12: e1005871","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2018101365_CR15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.immuni.2017.11.013","volume":"48","author":"CL Evavold","year":"2018","unstructured":"Evavold CL, Ruan J, Tan Y, Xia S, Wu H, Kagan JC (2018) The pore\u2010forming protein gasdermin D regulates interleukin\u20101 secretion from living macrophages. Immunity 48: 35\u201344.e6","journal-title":"Immunity"},{"key":"BFEMBJ2018101365_CR16","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/ni.2913","volume":"15","author":"BS Franklin","year":"2014","unstructured":"Franklin BS, Bossaller L, De Nardo D, Ratter JM, Stutz A, Engels G, Brenker C, Nordhoff M, Mirandola SR, Al\u2010Amoudi A et\u00a0al (2014) The adaptor ASC has extracellular and \u2018prionoid\u2019 activities that propagate inflammation. Nat Immunol 15: 727\u2013737","journal-title":"Nat Immunol"},{"key":"BFEMBJ2018101365_CR17","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/j.cell.2017.09.039","volume":"171","author":"MM Gaidt","year":"2017","unstructured":"Gaidt MM, Ebert TS, Chauhan D, Ramshorn K, Pinci F, Zuber S, O'Duill F, Schmid\u2010Burgk JL, Hoss F, Buhmann R et\u00a0al (2017) The DNA inflammasome in human myeloid cells is initiated by a STING\u2010cell death program upstream of NLRP3. Cell 171: 1110\u20131124.e18","journal-title":"Cell"},{"key":"BFEMBJ2018101365_CR18","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.jmb.2017.11.013","volume":"430","author":"MM Gaidt","year":"2018","unstructured":"Gaidt MM, Hornung V (2018) The NLRP3 inflammasome renders cell death pro\u2010inflammatory. J Mol Biol 430: 133\u2013141","journal-title":"J Mol Biol"},{"key":"BFEMBJ2018101365_CR19","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.1128\/JVI.02568-15","volume":"90","author":"UF Greber","year":"2016","unstructured":"Greber UF (2016) Virus and host mechanics support membrane penetration and cell entry. J Virol 90: 3802\u20133805","journal-title":"J Virol"},{"key":"BFEMBJ2018101365_CR20","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/s41586-018-0287-8","volume":"559","author":"SM Haag","year":"2018","unstructured":"Haag SM, Gulen MF, Reymond L, Gibelin A, Abrami L, Decout A, Heymann M, van der Goot FG, Turcatti G, Behrendt R et\u00a0al (2018) Targeting STING with covalent small\u2010molecule inhibitors. Nature 559: 269\u2013273","journal-title":"Nature"},{"key":"BFEMBJ2018101365_CR21","doi-asserted-by":"crossref","first-page":"19733","DOI":"10.1073\/pnas.1210659109","volume":"109","author":"F Hauler","year":"2012","unstructured":"Hauler F, Mallery DL, McEwan WA, Bidgood SR, James LC (2012) AAA ATPase p97\/VCP is essential for TRIM21\u2010mediated virus neutralization. Proc Natl Acad Sci 109: 19733\u201319738","journal-title":"Proc Natl Acad Sci"},{"key":"BFEMBJ2018101365_CR22","doi-asserted-by":"crossref","first-page":"e29443","DOI":"10.1371\/journal.pone.0029443","volume":"7","author":"MA Hoeve","year":"2012","unstructured":"Hoeve MA, Nash AA, Jackson D, Randall RE, Dransfield I (2012) Influenza virus A infection of human monocyte and macrophage subpopulations reveals increased susceptibility associated with cell differentiation. PLoS ONE 7: e29443","journal-title":"PLoS ONE"},{"key":"BFEMBJ2018101365_CR23","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-62703-523-1_9","volume-title":"The Inflammasome: Methods and Protocols","author":"C Jakobs","year":"2013","unstructured":"Jakobs C, Bartok E, Kubarenko A, Bauernfeind F, Hornung V (2013) Immunoblotting for active Caspase\u20101. In The Inflammasome: Methods and Protocols, De Nardo CM, Latz E (eds), pp 103\u2013115. Totowa, NJ: Humana Press"},{"key":"BFEMBJ2018101365_CR24","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/nri.2016.147","volume":"17","author":"I Jorgensen","year":"2017","unstructured":"Jorgensen I, Rayamajhi M, Miao EA (2017) Programmed cell death as a defence against infection. Nat Rev Immunol 17: 151\u2013164","journal-title":"Nat Rev Immunol"},{"key":"BFEMBJ2018101365_CR25","first-page":"328","volume":"2","author":"A Klepper","year":"2015","unstructured":"Klepper A, Branch AD (2015) Macrophages and the viral dissemination super highway. EC Microbiol 2: 328\u2013336","journal-title":"EC Microbiol"},{"key":"BFEMBJ2018101365_CR26","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.cell.2014.04.007","volume":"157","author":"M Lamkanfi","year":"2014","unstructured":"Lamkanfi M, Dixit VM (2014) Mechanisms and functions of inflammasomes. Cell 157: 1013\u20131022","journal-title":"Cell"},{"key":"BFEMBJ2018101365_CR27","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nri.2017.106","volume":"18","author":"LL Lu","year":"2017","unstructured":"Lu LL, Suscovich TJ, Fortune SM, Alter G (2017) Beyond binding: antibody effector functions in infectious diseases. Nat Rev Immunol 18: 46\u201361","journal-title":"Nat Rev Immunol"},{"key":"BFEMBJ2018101365_CR28","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1038\/ncomms2963","volume":"4","author":"DT MacLeod","year":"2013","unstructured":"MacLeod DT, Nakatsuji T, Yamasaki K, Kobzik L, Gallo RL (2013) HSV\u20101 exploits the innate immune scavenger receptor MARCO to enhance epithelial adsorption and infection. Nat Commun 4: 1963","journal-title":"Nat Commun"},{"key":"BFEMBJ2018101365_CR29","doi-asserted-by":"crossref","first-page":"19985","DOI":"10.1073\/pnas.1014074107","volume":"107","author":"DL Mallery","year":"2010","unstructured":"Mallery DL, McEwan WA, Bidgood SR, Towers GJ, Johnson CM, James LC (2010) Antibodies mediate intracellular immunity through tripartite motif\u2010containing 21 (TRIM21). Proc Natl Acad Sci 107: 19985\u201319990","journal-title":"Proc Natl Acad Sci"},{"key":"BFEMBJ2018101365_CR30","doi-asserted-by":"crossref","first-page":"956","DOI":"10.4049\/jimmunol.1502060","volume":"196","author":"VI Maltez","year":"2016","unstructured":"Maltez VI, Miao EA (2016) Reassessing the evolutionary importance of inflammasomes. J Immunol 196: 956\u2013962","journal-title":"J Immunol"},{"key":"BFEMBJ2018101365_CR31","doi-asserted-by":"crossref","first-page":"e01417\u201016","DOI":"10.1128\/JVI.01417-16","volume":"91","author":"SA Marvin","year":"2017","unstructured":"Marvin SA, Russier M, Huerta CT, Russell CJ, Schultz\u2010Cherry S (2017) Influenza virus overcomes cellular blocks to productively replicate, impacting macrophage function. J Virol 91: e01417\u201016","journal-title":"J Virol"},{"key":"BFEMBJ2018101365_CR32","doi-asserted-by":"crossref","first-page":"8482","DOI":"10.1128\/JVI.00728-12","volume":"86","author":"WA McEwan","year":"2012","unstructured":"McEwan WA, Hauler F, Williams CR, Bidgood SR, Mallery DL, Crowther RA, James LC (2012) Regulation of virus neutralization and the persistent fraction by TRIM21. J Virol 86: 8482\u20138491","journal-title":"J Virol"},{"key":"BFEMBJ2018101365_CR33","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/ni.2548","volume":"14","author":"WA McEwan","year":"2013","unstructured":"McEwan WA, Tam JCH, Watkinson RE, Bidgood SR, Mallery DL, James LC (2013) Intracellular antibody\u2010bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat Immunol 14: 327\u2013336","journal-title":"Nat Immunol"},{"key":"BFEMBJ2018101365_CR34","doi-asserted-by":"crossref","first-page":"E2142","DOI":"10.1073\/pnas.1513943113","volume":"113","author":"AJ Monteith","year":"2016","unstructured":"Monteith AJ, Kang S, Scott E, Hillman K, Rajfur Z, Jacobson K, Costello MJ, Vilen BJ (2016) Defects in lysosomal maturation facilitate the activation of innate sensors in systemic lupus erythematosus. Proc Natl Acad Sci 113: E2142\u2013E2151","journal-title":"Proc Natl Acad Sci"},{"key":"BFEMBJ2018101365_CR35","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/j.celrep.2018.07.027","volume":"24","author":"M Monteleone","year":"2018","unstructured":"Monteleone M, Stanley AC, Chen KW, Brown DL, Bezbradica JS, von Pein JB, Holley CL, Boucher D, Shakespear MR, Kapetanovic R et\u00a0al (2018) Interleukin\u20101\u03b2 maturation triggers its relocation to the plasma membrane for gasdermin\u2010D\u2010dependent and \u2010independent secretion. Cell Rep 24: 1425\u20131433","journal-title":"Cell Rep"},{"key":"BFEMBJ2018101365_CR36","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/nature06664","volume":"452","author":"DA Muruve","year":"2008","unstructured":"Muruve DA, P\u00e9trilli V, Zaiss AK, White LR, Clark SA, Ross PJ, Parks RJ, Tschopp J (2008) The inflammasome recognizes cytosolic microbial and host DNA and triggers an innate immune response. Nature 452: 103\u2013107","journal-title":"Nature"},{"key":"BFEMBJ2018101365_CR37","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.immuni.2014.10.015","volume":"41","author":"JV Price","year":"2014","unstructured":"Price JV, Vance RE (2014) The macrophage paradox. Immunity 41: 685\u2013693","journal-title":"Immunity"},{"key":"BFEMBJ2018101365_CR38","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/nature12769","volume":"503","author":"J Rasaiyaah","year":"2013","unstructured":"Rasaiyaah J, Tan CP, Fletcher AJ, Price AJ, Blondeau C, Hilditch L, Jacques DA, Selwood DL, James LC, Noursadeghi M et\u00a0al (2013) HIV\u20101 evades innate immune recognition through specific cofactor recruitment. Nature 503: 402\u2013405","journal-title":"Nature"},{"key":"BFEMBJ2018101365_CR39","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1093\/emboj\/20.9.2140","volume":"20","author":"A Reymond","year":"2001","unstructured":"Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S et\u00a0al (2001) The tripartite motif family identifies cell compartments. EMBO J 20: 2140\u20132151","journal-title":"EMBO J"},{"key":"BFEMBJ2018101365_CR40","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1002\/eji.201545523","volume":"45","author":"JL Schmid\u2010Burgk","year":"2015","unstructured":"Schmid\u2010Burgk JL, Gaidt MM, Schmidt T, Ebert TS, Bartok E, Hornung V (2015) Caspase\u20104 mediates non\u2010canonical activation of the NLRP3 inflammasome in human myeloid cells. Eur J Immunol 45: 2911\u20132917","journal-title":"Eur J Immunol"},{"key":"BFEMBJ2018101365_CR41","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3389\/fimmu.2018.00017","volume":"9","author":"D\u2010W Shim","year":"2018","unstructured":"Shim D\u2010W, Lee K\u2010H (2018) Posttranslational regulation of the NLR family pyrin domain\u2010containing 3 inflammasome. Front Immunol 9: 17\u201319","journal-title":"Front Immunol"},{"key":"BFEMBJ2018101365_CR42","doi-asserted-by":"crossref","first-page":"e1006914","DOI":"10.1371\/journal.ppat.1006914","volume":"14","author":"N Stichling","year":"2018","unstructured":"Stichling N, Suomalainen M, Flatt JW, Schmid M, Pacesa M, Hemmi S, Jungraithmayr W, Maler MD, Freudenberg MA, Pl\u00fcckthun A et\u00a0al (2018) Lung macrophage scavenger receptor SR\u2010A6 (MARCO) is an adenovirus type\u2010specific virus entry receptor. PLoS Pathog 14: e1006914","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2018101365_CR43","doi-asserted-by":"crossref","first-page":"10354","DOI":"10.1128\/JVI.00936-14","volume":"88","author":"JE Teigler","year":"2014","unstructured":"Teigler JE, Kagan JC, Barouch DH (2014) Late endosomal trafficking of alternative serotype adenovirus vaccine vectors augments antiviral innate immunity. J Virol 88: 10354\u201310363","journal-title":"J Virol"},{"key":"BFEMBJ2018101365_CR44","doi-asserted-by":"crossref","first-page":"12397","DOI":"10.1073\/pnas.1301918110","volume":"110","author":"M Vaysburd","year":"2013","unstructured":"Vaysburd M, Watkinson RE, Cooper H, Reed M, O'Connell K, Smith J, Cruickshanks J, James LC (2013) Intracellular antibody receptor TRIM21 prevents fatal viral infection. Proc Natl Acad Sci 110: 12397\u201312401","journal-title":"Proc Natl Acad Sci"},{"key":"BFEMBJ2018101365_CR45","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.chom.2015.05.003","volume":"17","author":"R Wassermann","year":"2015","unstructured":"Wassermann R, Gulen MF, Sala C, Perin SG, Lou Y, Rybniker J, Schmid\u2010Burgk JL, Schmidt T, Hornung V, Cole ST et\u00a0al (2015) Mycobacterium tuberculosis differentially activates cGAS\u2010 and inflammasome\u2010dependent intracellular immune responses through ESX\u20101. Cell Host Microbe 17: 799\u2013810","journal-title":"Cell Host Microbe"},{"key":"BFEMBJ2018101365_CR46","doi-asserted-by":"crossref","first-page":"e1005253","DOI":"10.1371\/journal.ppat.1005253","volume":"11","author":"RE Watkinson","year":"2015","unstructured":"Watkinson RE, McEwan WA, Tam JCH, Vaysburd M, James LC (2015)\u00a0TRIM21 promotes cGAS and RIG\u2010I sensing of viral genomes during infection by antibody\u2010opsonized virus. PLoS Pathog 11: e1005253","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2018101365_CR47","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.3389\/fimmu.2018.01664","volume":"9","author":"V Westenius","year":"2018","unstructured":"Westenius V, M\u00e4kel\u00e4 SM, Julkunen I, \u00d6sterlund P (2018) Highly pathogenic H5N1 influenza A virus spreads efficiently in human primary monocyte\u2010derived macrophages and dendritic cells. Front Immunol 9: 1664","journal-title":"Front Immunol"},{"key":"BFEMBJ2018101365_CR48","doi-asserted-by":"crossref","first-page":"7058","DOI":"10.4049\/jimmunol.0804269","volume":"182","author":"AK Zaiss","year":"2009","unstructured":"Zaiss AK, Vilaysane A, Cotter MJ, Clark SA, Meijndert HC, Colarusso P, Yates RM, Petrilli V, Tschopp J, Muruve DA (2009) Antiviral antibodies target adenovirus to phagolysosomes and amplify the innate immune response. J\u00a0Immunol 182: 7058\u20137068","journal-title":"J\u00a0Immunol"}],"container-title":["The EMBO Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embj.2018101365.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.15252\/embj.2018101365","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/embj.2018101365\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/embj.2018101365","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embj.2018101365.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T19:59:48Z","timestamp":1779307188000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/embj.2018101365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,29]]},"references-count":48,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2019,11,4]]}},"alternative-id":["BFEMBJ2018101365"],"URL":"https:\/\/doi.org\/10.15252\/embj.2018101365","relation":{},"ISSN":["0261-4189","1460-2075"],"issn-type":[{"value":"0261-4189","type":"print"},{"value":"1460-2075","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,29]]},"assertion":[{"value":"13 December 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 July 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 July 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2019","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"EMBJ2018101365"}}