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While the cellular processes underlying synapse formation have been well characterized, those that maintain the symmetry, and thereby the stability of the synapse, remain unknown. Here we identify an antigen\u2010triggered mechanism that actively promotes T\u2010cell synapse symmetry by generating cytoskeletal tension in the plane of the synapse through focal nucleation of actin via Wiskott\u2013Aldrich syndrome protein (WASP), and contraction of the resultant actin filaments by myosin II. Following T\u2010cell activation, WASP is degraded, leading to cytoskeletal unraveling and tension decay, which result in synapse breaking. Thus, our study identifies and characterizes a mechanical program within otherwise highly motile T cells that sustains the symmetry and stability of the T cell\u2013APC synaptic contact.<\/jats:p>","DOI":"10.15252\/embj.2019102783","type":"journal-article","created":{"date-parts":[[2020,1,2]],"date-time":"2020-01-02T08:32:20Z","timestamp":1577953940000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Cytoskeletal tension actively sustains the migratory T\u2010cell synaptic contact"],"prefix":"10.1038","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7082-2825","authenticated-orcid":false,"given":"Sudha","family":"Kumari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3510-6369","authenticated-orcid":false,"given":"Yeh\u2010Chuin","family":"Poh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mira","family":"Tohme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicki","family":"Watson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariane","family":"Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Janssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4983-6389","authenticated-orcid":false,"given":"Michael","family":"Dustin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raif","family":"Geha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8637-1405","authenticated-orcid":false,"given":"Darrell J","family":"Irvine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,2]]},"reference":[{"key":"BFEMBJ2019102783_CR1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1083\/jcb.201201018","volume":"197","author":"A Babich","year":"2012","unstructured":"Babich A, Li S, O'Connor RS, Milone MC, Freedman BD, Burkhardt JK (2012) F\u2010actin polymerization and retrograde flow drive sustained PLCgamma1 signaling during T cell activation. 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