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The mechanisms enabling this functional diversity remain elusive. Here, we asked whether the sound intensity information, contained in the receptor potential of the presynaptic inner hair cell (IHC), is fractionated via heterogeneous synapses. We studied the transfer function of individual IHC synapses by combining patch\u2010clamp recordings with dual\u2010color Rhod\u2010FF and iGluSnFR imaging of presynaptic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals and glutamate release. Synapses differed in the voltage dependence of release: Those residing at the IHC' pillar side activated at more hyperpolarized potentials and typically showed tight control of release by few Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels. We conclude that heterogeneity of voltage dependence and release site coupling of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels among the synapses varies synaptic transfer within individual IHCs and, thereby, likely contributes to the functional diversity of SGNs. The mechanism reported here might serve sensory cells and neurons more generally to diversify signaling even in close\u2010by synapses.\n                  <\/jats:p>","DOI":"10.15252\/embj.2020106010","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T20:42:07Z","timestamp":1608583327000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["A sensory cell diversifies its output by varying Ca2+ influx\u2010release coupling among active zones"],"prefix":"10.1038","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0717-7936","authenticated-orcid":false,"given":"\u00d6zge D","family":"\u00d6z\u00e7ete","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7145-0533","authenticated-orcid":false,"given":"Tobias","family":"Moser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,21]]},"reference":[{"key":"BFEMBJ2020106010_CR400","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.neuron.2012.05.019","volume":"75","author":"O Akil","year":"2012","unstructured":"Akil O, Seal RP, Burke K, Wang C, Alemi A, During M, Edwards RH, Lustig LR (2012) Restoration of hearing in the VGLUT3 knockout mouse using virally mediated gene therapy. 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