{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:29:59Z","timestamp":1790972999903,"version":"4.1.0"},"reference-count":98,"publisher":"Springer Science and Business Media LLC","issue":"22","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201011\u2010LABX\u20100024"],"award-info":[{"award-number":["ANR\u201011\u2010LABX\u20100024"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000008","name":"David and Lucile Packard Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000008","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","award":["833309"],"award-info":[{"award-number":["833309"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH GM105783"],"award-info":[{"award-number":["NIH GM105783"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R01 AI112427"],"award-info":[{"award-number":["NIH R01 AI112427"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO J"],"published-print":{"date-parts":[[2022,11,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Apicomplexan parasites possess secretory organelles called rhoptries that undergo regulated exocytosis upon contact with the host. This process is essential for the parasitic lifestyle of these pathogens and relies on an exocytic machinery sharing structural features and molecular components with free\u2010living ciliates. However, how the parasites coordinate exocytosis with host interaction is unknown. Here, we performed a\n                    <jats:italic>Tetrahymena<\/jats:italic>\n                    \u2010based transcriptomic screen to uncover novel exocytic factors in Ciliata and\u00a0conserved in Apicomplexa. We identified membrane\u2010bound proteins, named CRMPs, forming part of a large complex essential for rhoptry secretion and invasion in\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    . Using cutting\u2010edge imaging tools, including expansion microscopy and cryo\u2010electron tomography, we show that, unlike previously described rhoptry exocytic factors, TgCRMPs are not required for the assembly of the rhoptry secretion machinery and only transiently associate with the exocytic site\u2014prior to the invasion. CRMPs and their partners contain putative host cell\u2010binding domains, and CRMPa shares similarities with GPCR proteins. Collectively our data imply that the CRMP complex acts as a host\u2013molecular sensor to ensure that rhoptry exocytosis occurs when the parasite contacts the host\u00a0cell.\n                  <\/jats:p>","DOI":"10.15252\/embj.2022111158","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T03:27:18Z","timestamp":1665977238000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma"],"prefix":"10.1038","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5992-3753","authenticated-orcid":false,"given":"Daniela","family":"Sparvoli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Delabre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Marcela","family":"Penarete\u2010Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrawan","family":"Kumar Mageswaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0642-8468","authenticated-orcid":false,"given":"Lev M","family":"Tsypin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine","family":"Heckendorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam","family":"Theveny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1825-3095","authenticated-orcid":false,"given":"Marjorie","family":"Maynadier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6727-248X","authenticated-orcid":false,"given":"Marta","family":"Mendon\u00e7a Cova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Berry\u2010Sterkers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Gu\u00e9rin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Dubremetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8663-2006","authenticated-orcid":false,"given":"Serge","family":"Urbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7426-432X","authenticated-orcid":false,"given":"Boris","family":"Striepen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron P","family":"Turkewitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2391-473X","authenticated-orcid":false,"given":"Yi\u2010Wei","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-8408","authenticated-orcid":false,"given":"Maryse","family":"Lebrun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"BFEMBJ2022111158_CR1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0166-6851(91)90182-6","volume":"47","author":"A Achbarou","year":"1991","unstructured":"Achbarou A, Mercereau\u2010Puijalon O, Autheman JM, Fortier B, Camus D, Dubremetz JF (1991a) Characterization of microneme proteins of Toxoplasma gondii. Mol Biochem Parasitol 47: 223\u2013233","journal-title":"Mol Biochem Parasitol"},{"key":"BFEMBJ2022111158_CR2","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1017\/S0031182000059837","volume":"3","author":"A Achbarou","year":"1991","unstructured":"Achbarou A, Mercereau\u2010Puijalon O, Sadak A, Fortier B, Leriche MA, Camus D, Dubremetz JF (1991b) Differential targeting of dense granule proteins in the parasitophorous vacuole of Toxoplasma gondii. Parasitology 3: 321\u2013329","journal-title":"Parasitology"},{"key":"BFEMBJ2022111158_CR3","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1002\/(SICI)1097-0177(200006)218:2<280::AID-DVDY4>3.0.CO;2-0","volume":"218","author":"JC Adams","year":"2000","unstructured":"Adams JC, Tucker RP (2000) The thrombospondin type 1 repeat (TSR) superfamily: diverse proteins with related roles in neuronal development. Dev Dyn 218: 280\u2013299","journal-title":"Dev Dyn"},{"key":"BFEMBJ2022111158_CR4","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/s41564-020-00854-z","volume":"6","author":"E Aquilini","year":"2021","unstructured":"Aquilini E, Cova MM, Mageswaran SK, Dos Santos Pacheco N, Sparvoli D, Penarete\u2010Vargas DM, Najm R, Graindorge A, Suarez C, Maynadier M et\u00a0al (2021) An Alveolata secretory machinery adapted to parasite host cell invasion. Nat Microbiol 6: 425\u2013434","journal-title":"Nat Microbiol"},{"key":"BFEMBJ2022111158_CR5","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","volume":"28","author":"K Barylyuk","year":"2020","unstructured":"Barylyuk K, Koreny L, Ke H, Butterworth S, Crook OM, Lassadi I, Gupta V, Tromer E, Mourier T, Stevens TJ et\u00a0al (2020) A comprehensive subcellular atlas of the toxoplasma proteome via hyperLOPIT provides spatial context for protein functions. Cell Host Microbe 28: 752\u2013766.e9","journal-title":"Cell Host Microbe"},{"key":"BFEMBJ2022111158_CR6","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0166-6851(96)02598-4","volume":"77","author":"P Bastin","year":"1996","unstructured":"Bastin P, Bagherzadeh Z, Matthews KR, Gull K (1996) A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei. Mol Biochem Parasitol 77: 235\u2013239","journal-title":"Mol Biochem Parasitol"},{"key":"BFEMBJ2022111158_CR7","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1083\/jcb.69.1.126","volume":"69","author":"J Beisson","year":"1976","unstructured":"Beisson J, Lefort\u2010Tran M, Pouphile M, Rossignol M, Satir B (1976) Genetic analysis of membrane differentiation in paramecium. Freeze\u2010fracture study of the trichocyst cycle in wild\u2010type and mutant strains. J Cell Biol 69: 126\u2013143","journal-title":"J Cell Biol"},{"key":"BFEMBJ2022111158_CR8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000309","volume":"5","author":"S Besteiro","year":"2009","unstructured":"Besteiro S, Michelin A, Poncet J, Dubremetz J\u2010F, Lebrun M (2009) Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasion. PLoS Pathog 5: e1000309","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR9","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1111\/j.1462-5822.2011.01597.x","volume":"13","author":"S Besteiro","year":"2011","unstructured":"Besteiro S, Dubremetz JF, Lebrun M (2011) The moving junction of apicomplexan parasites: a key structure for invasion. Cell Microbiol 13: 797\u2013805","journal-title":"Cell Microbiol"},{"key":"BFEMBJ2022111158_CR10","doi-asserted-by":"crossref","first-page":"2808","DOI":"10.1038\/sj.emboj.7601704","volume":"26","author":"TM Blumenschein","year":"2007","unstructured":"Blumenschein TM, Friedrich N, Childs RA, Saouros S, Carpenter EP, Campanero\u2010Rhodes MA, Simpson P, Chai W, Koutroukides T, Blackman MJ et\u00a0al (2007) Atomic resolution insight into host cell recognition by Toxoplasma gondii. EMBO J 26: 2808\u20132820","journal-title":"EMBO J"},{"key":"BFEMBJ2022111158_CR11","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-2818.2006.01706.x","volume":"224","author":"S Bolte","year":"2006","unstructured":"Bolte S, Cordelieres FP (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 224: 213\u2013232","journal-title":"J Microsc"},{"key":"BFEMBJ2022111158_CR12","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1083\/jcb.201305086","volume":"203","author":"JS Briguglio","year":"2013","unstructured":"Briguglio JS, Kumar S, Turkewitz AP (2013) Lysosomal sorting receptors are essential for secretory granule biogenesis in Tetrahymena. J Cell Biol 203: 537\u2013550","journal-title":"J Cell Biol"},{"key":"BFEMBJ2022111158_CR13","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00375-17","volume":"8","author":"KM Brown","year":"2017","unstructured":"Brown KM, Long S, Sibley LD (2017) Plasma membrane association by N\u2010acylation governs PKG function in Toxoplasma gondii. mBio 8: e00375\u201017","journal-title":"mBio"},{"key":"BFEMBJ2022111158_CR14","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.2728","volume":"8","author":"KM Brown","year":"2018","unstructured":"Brown KM, Long S, Sibley LD (2018) Conditional knockdown of proteins using auxin\u2010inducible degron (AID) fusions in Toxoplasma gondii. Bio Protoc 8: e2728","journal-title":"Bio Protoc"},{"key":"BFEMBJ2022111158_CR15","volume":"18","author":"A Burrell","year":"2021","unstructured":"Burrell A, Marugan\u2010Hernandez V, Moreira\u2010Leite F, Ferguson DJP, Tomley F, Vaughan S (2021) Cellular electron tomography of the apical complex 1 in the apicomplexan 2 parasite Eimeria tenella shows a highly organised gateway for regulated secretion. PLoS Pathog 18: e1010666","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR16","first-page":"114","volume":"73","author":"VB Carruthers","year":"1997","unstructured":"Carruthers VB, Sibley LD (1997) Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts. Eur J Cell Biol 73: 114\u2013123","journal-title":"Eur J Cell Biol"},{"key":"BFEMBJ2022111158_CR17","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1046\/j.1365-2958.1999.01174.x","volume":"31","author":"VB Carruthers","year":"1999","unstructured":"Carruthers VB, Sibley LD (1999) Mobilization of intracellular calcium stimulates microneme discharge in Toxoplasma gondii. Mol Microbiol 31: 421\u2013428","journal-title":"Mol Microbiol"},{"key":"BFEMBJ2022111158_CR18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/genetics\/146.1.135","volume":"146","author":"D Cassidy\u2010Hanley","year":"1997","unstructured":"Cassidy\u2010Hanley D, Bowen J, Lee JH, Cole E, VerPlank LA, Gaertig J, Gorovsky MA, Bruns PJ (1997) Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics 146: 135\u2013147","journal-title":"Genetics"},{"key":"BFEMBJ2022111158_CR19","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1084\/jem.20041672","volume":"201","author":"O Cerede","year":"2005","unstructured":"Cerede O, Dubremetz JF, Soete M, Deslee D, Vial H, Bout D, Lebrun M (2005) Synergistic role of micronemal proteins in Toxoplasma gondii virulence. J Exp Med 201: 453\u2013463","journal-title":"J Exp Med"},{"key":"BFEMBJ2022111158_CR20","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1083\/jcb.135.6.1775","volume":"135","author":"ND Chilcoat","year":"1996","unstructured":"Chilcoat ND, Melia SM, Haddad A, Turkewitz AP (1996) Granule lattice protein 1 (Grl1p), an acidic, calcium\u2010binding protein in Tetrahymena thermophila dense\u2010core secretory granules, influences granule size, shape, content organization, and release but not protein sorting or condensation. J Cell Biol 135: 1775\u20131787","journal-title":"J Cell Biol"},{"key":"BFEMBJ2022111158_CR21","volume":"9","author":"BI Coleman","year":"2018","unstructured":"Coleman BI, Saha S, Sato S, Engelberg K, Ferguson DJP, Coppens I, Lodoen MB, Gubbels MJ (2018) A member of the ferlin calcium sensor family is essential for Toxoplasma gondii rhoptry secretion. mBio 9: e01510\u201018","journal-title":"mBio"},{"issue":"Pt 1","key":"BFEMBJ2022111158_CR22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182000066695","volume":"97","author":"G Couvreur","year":"1988","unstructured":"Couvreur G, Sadak A, Fortier B & Dubremetz JF (1988) Surface antigens of Toxoplasma gondii. Parasitology 97 (Pt 1): 1\u201310","journal-title":"Parasitology"},{"key":"BFEMBJ2022111158_CR23","doi-asserted-by":"crossref","first-page":"4046","DOI":"10.1091\/mbc.e05-01-0028","volume":"16","author":"AT Cowan","year":"2005","unstructured":"Cowan AT, Bowman GR, Edwards KF, Emerson JJ, Turkewitz AP (2005) Genetic, genomic, and functional analysis of the granule lattice proteins in Tetrahymena secretory granules. Mol Biol Cell 16: 4046\u20134060","journal-title":"Mol Biol Cell"},{"key":"BFEMBJ2022111158_CR24","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","volume":"26","author":"J Cox","year":"2008","unstructured":"Cox J, Mann M (2008) MaxQuant enables high peptide identification rates, individualized p.p.b.\u2010range mass accuracies and proteome\u2010wide protein quantification. Nat Biotechnol 26: 1367\u20131372","journal-title":"Nat Biotechnol"},{"key":"BFEMBJ2022111158_CR25","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1021\/pr101065j","volume":"10","author":"J Cox","year":"2011","unstructured":"Cox J, Neuhauser N, Michalski A, Scheltema RA, Olsen JV, Mann M (2011) Andromeda: a peptide search engine integrated into the MaxQuant environment. J Proteome Res 10: 1794\u20131805","journal-title":"J Proteome Res"},{"key":"BFEMBJ2022111158_CR26","doi-asserted-by":"crossref","first-page":"15635","DOI":"10.1073\/pnas.1418377111","volume":"111","author":"R Danev","year":"2014","unstructured":"Danev R, Buijsse B, Khoshouei M, Plitzko JM, Baumeister W (2014) Volta potential phase plate for in\u2010focus phase contrast transmission electron microscopy. Proc Natl Acad Sci U S A 111: 15635\u201315640","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BFEMBJ2022111158_CR27","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/1475-2875-10-71","volume":"10","author":"B Douradinha","year":"2011","unstructured":"Douradinha B, Augustijn KD, Moore SG, Ramesar J, Mota MM, Waters AP, Janse CJ, Thompson J (2011) Plasmodium cysteine repeat modular proteins 3 and 4 are essential for malaria parasite transmission from the mosquito to the host. Malar J 10: 71","journal-title":"Malar J"},{"key":"BFEMBJ2022111158_CR28","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0966-842X(97)01165-7","volume":"6","author":"JF Dubremetz","year":"1998","unstructured":"Dubremetz JF (1998) Host cell invasion by Toxoplasma gondii. Trends Microbiol 6: 27\u201330","journal-title":"Trends Microbiol"},{"key":"BFEMBJ2022111158_CR29","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/j.1462-5822.2006.00767.x","volume":"9","author":"H El Hajj","year":"2007","unstructured":"El Hajj H, Lebrun M, Fourmaux MN, Vial H, Dubremetz JF (2007) Inverted topology of the Toxoplasma gondii ROP5 rhoptry protein provides new insights into the association of the ROP2 protein family with the parasitophorous vacuole membrane. Cell Microbiol 9: 54\u201364","journal-title":"Cell Microbiol"},{"key":"BFEMBJ2022111158_CR30","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1128\/EC.00413-07","volume":"7","author":"H El Hajj","year":"2008","unstructured":"El Hajj H, Papoin J, C\u00e9r\u00e8de O, Garcia\u2010R\u00e9guet N, So\u00eate M, Dubremetz J\u2010F, Lebrun M (2008) Molecular signals in the trafficking of Toxoplasma gondii protein MIC3 to the micronemes. Eukaryot Cell 7: 1019\u20131028","journal-title":"Eukaryot Cell"},{"key":"BFEMBJ2022111158_CR31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010643","volume":"18","author":"P Fernandes","year":"2022","unstructured":"Fernandes P, Loubens M, Le Borgne R, Marinach C, Ardin B, Briquet S, Vincensini L, Hamada S, Hoareau\u2010Coudert B, Verbavatz J\u2010M et\u00a0al (2022) The AMA1\u2010RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts. PLoS Pathog 18: e1010643","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR32","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1124\/mol.104.009001","volume":"67","author":"R Fredriksson","year":"2005","unstructured":"Fredriksson R, Schi\u00f6th HB (2005) The repertoire of G\u2010protein\u2010coupled receptors in fully sequenced genomes. Mol Pharmacol 67: 1414\u20131425","journal-title":"Mol Pharmacol"},{"key":"BFEMBJ2022111158_CR33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004504","volume":"10","author":"K Frenal","year":"2014","unstructured":"Frenal K, Marq JB, Jacot D, Polonais V, Soldati\u2010Favre D (2014) Plasticity between MyoC\u2010 and MyoA\u2010glideosomes: an example of functional compensation in Toxoplasma gondii invasion. PLoS Pathog 10: e1004504","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR34","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1074\/jbc.M109.060988","volume":"285","author":"N Friedrich","year":"2010","unstructured":"Friedrich N, Santos JM, Liu Y, Palma AS, Leon E, Saouros S, Kiso M, Blackman MJ, Matthews S, Feizi T et\u00a0al (2010) Members of a novel protein family containing microneme adhesive repeat domains act as sialic acid\u2010binding lectins during host cell invasion by apicomplexan parasites. J Biol Chem 285: 2064\u20132076","journal-title":"J Biol Chem"},{"key":"BFEMBJ2022111158_CR35","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1093\/genetics\/157.2.611","volume":"157","author":"M Froissard","year":"2001","unstructured":"Froissard M, Keller AM, Cohen J (2001) ND9P, a novel protein with armadillo\u2010like repeats involved in exocytosis: physiological studies using allelic mutants in paramecium. Genetics 157: 611\u2013620","journal-title":"Genetics"},{"key":"BFEMBJ2022111158_CR36","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jsb.2015.03.004","volume":"190","author":"Y Fukuda","year":"2015","unstructured":"Fukuda Y, Laugks U, Lu\u010di\u0107 V, Baumeister W, Danev R (2015) Electron cryotomography of vitrified cells with a Volta phase plate. J Struct Biol 190: 143\u2013154","journal-title":"J Struct Biol"},{"key":"BFEMBJ2022111158_CR37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/cmi.12733","volume":"19","author":"S Ghosh","year":"2017","unstructured":"Ghosh S, Kennedy K, Sanders P, Matthews K, Ralph SA, Counihan NA, de Koning\u2010Ward TF (2017) The Plasmodium rhoptry associated protein complex is important for parasitophorous vacuole membrane structure and intraerythrocytic parasite growth. Cell Microbiol 19: 1\u201316","journal-title":"Cell Microbiol"},{"key":"BFEMBJ2022111158_CR38","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/EC.00309-06","volume":"6","author":"LA Gilbert","year":"2007","unstructured":"Gilbert LA, Ravindran S, Turetzky JM, Boothroyd JC, Bradley PJ (2007) Toxoplasma gondii targets a protein phosphatase 2C to the nuclei of infected host cells. Eukaryot Cell 6: 73\u201383","journal-title":"Eukaryot Cell"},{"key":"BFEMBJ2022111158_CR39","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1128\/EC.4.12.2129-2139.2005","volume":"4","author":"D Gogendeau","year":"2005","unstructured":"Gogendeau D, Keller AM, Yanagi A, Cohen J, Koll F (2005) Nd6p, a novel protein with RCC1\u2010like domains involved in exocytosis in Paramecium tetraurelia. Eukaryot Cell 4: 2129\u20132139","journal-title":"Eukaryot Cell"},{"key":"BFEMBJ2022111158_CR40","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1038\/s41564-017-0018-1","volume":"2","author":"A Guerin","year":"2017","unstructured":"Guerin A, Corrales RM, Parker ML, Lamarque MH, Jacot D, El Hajj H, Soldati\u2010Favre D, Boulanger MJ, Lebrun M (2017) Efficient invasion by Toxoplasma depends on the subversion of host protein networks. Nat Microbiol 2: 1358\u20131366","journal-title":"Nat Microbiol"},{"key":"BFEMBJ2022111158_CR41","doi-asserted-by":"crossref","first-page":"10675","DOI":"10.1073\/pnas.94.20.10675","volume":"94","author":"A Haddad","year":"1997","unstructured":"Haddad A, Turkewitz AP (1997) Analysis of exocytosis mutants indicates close coupling between regulated secretion and transcription activation in Tetrahymena. Proc Natl Acad Sci U S A 94: 10675\u201310680","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BFEMBJ2022111158_CR42","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1128\/CMR.00005-17","volume":"30","author":"M\u2010A Hakimi","year":"2017","unstructured":"Hakimi M\u2010A, Olias P, Sibley LD (2017) Toxoplasma effectors targeting host signaling and transcription. Clin Microbiol Rev 30: 615\u2013645","journal-title":"Clin Microbiol Rev"},{"key":"BFEMBJ2022111158_CR43","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1111\/cmi.12132","volume":"15","author":"E Hanssen","year":"2013","unstructured":"Hanssen E, Dekiwadia C, Riglar DT, Rug M, Lemgruber L, Cowman AF, Cyrklaff M, Kudryashev M, Frischknecht F, Baum J et\u00a0al (2013) Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion. Cell Microbiol 15: 1457\u20131472","journal-title":"Cell Microbiol"},{"key":"BFEMBJ2022111158_CR44","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.cell.2017.11.033","volume":"172","author":"AS Hauser","year":"2018","unstructured":"Hauser AS, Chavali S, Masuho I, Jahn LJ, Martemyanov KA, Gloriam DE, Babu MM (2018) Pharmacogenomics of GPCR drug targets. Cell 172: 41\u201354.e19","journal-title":"Cell"},{"key":"BFEMBJ2022111158_CR45","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1128\/EC.00358-08","volume":"8","author":"M\u2010H Huynh","year":"2009","unstructured":"Huynh M\u2010H, Carruthers VB (2009) Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80. Eukaryot Cell 8: 530\u2013539","journal-title":"Eukaryot Cell"},{"key":"BFEMBJ2022111158_CR46","volume":"1","author":"M\u2010H Huynh","year":"2016","unstructured":"Huynh M\u2010H, Carruthers VB (2016) A Toxoplasma gondii ortholog of plasmodium GAMA contributes to parasite attachment and cell invasion. mSaphere 1: e00012\u201016","journal-title":"mSaphere"},{"key":"BFEMBJ2022111158_CR47","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.12964","volume":"21","author":"T Ishino","year":"2019","unstructured":"Ishino T, Murata E, Tokunaga N, Baba M, Tachibana M, Thongkukiatkul A, Tsuboi T, Torii M (2019) Rhoptry neck protein 2 expressed in Plasmodium sporozoites plays a crucial role during invasion of mosquito salivary glands. Cell Microbiol 21: e12964","journal-title":"Cell Microbiol"},{"key":"BFEMBJ2022111158_CR48","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/1574-6976.12013","volume":"37","author":"LE Kemp","year":"2012","unstructured":"Kemp LE, Yamamoto M, Soldati\u2010Favre D (2012) Subversion of host cellular functions by the apicomplexan parasites. FEMS Microbiol Rev 37: 607\u2013631","journal-title":"FEMS Microbiol Rev"},{"key":"BFEMBJ2022111158_CR49","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1242\/jcs.022350","volume":"121","author":"H Kessler","year":"2008","unstructured":"Kessler H, Herm\u2010Gotz A, Hegge S, Rauch M, Soldati\u2010Favre D, Frischknecht F, Meissner M (2008) Microneme protein 8: a new essential invasion factor in Toxoplasma gondii. J Cell Sci 121: 947\u2013956","journal-title":"J Cell Sci"},{"key":"BFEMBJ2022111158_CR50","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1126\/science.8235614","volume":"262","author":"K Kim","year":"1993","unstructured":"Kim K, Soldati D, Boothroyd JC (1993) Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science 262: 911\u2013914","journal-title":"Science"},{"key":"BFEMBJ2022111158_CR51","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/nmeth.1438","volume":"7","author":"AA Koshy","year":"2010","unstructured":"Koshy AA, Fouts AE, Lodoen MB, Alkan O, Blau HM, Boothroyd JC (2010) Toxoplasma secreting Cre recombinase for analysis of host\u2010parasite interactions. Nat Methods 7: 307\u2013309","journal-title":"Nat Methods"},{"key":"BFEMBJ2022111158_CR52","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","volume":"116","author":"JR Kremer","year":"1996","unstructured":"Kremer JR, Mastronarde DN, McIntosh JR (1996) Computer visualization of three\u2010dimensional image data using IMOD. J Struct Biol 116: 71\u201376","journal-title":"J Struct Biol"},{"key":"BFEMBJ2022111158_CR53","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003213","volume":"9","author":"K Kremer","year":"2013","unstructured":"Kremer K, Kamin D, Rittweger E, Wilkes J, Flammer H, Mahler S, Heng J, Tonkin CJ, Langsley G, Hell SW et\u00a0al (2013) An overexpression screen of Toxoplasma gondii Rab\u2010GTPases reveals distinct transport routes to the micronemes. PLoS Pathog 9: e1003213","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR54","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0304-3991(95)00034-X","volume":"59","author":"OL Krivanek","year":"1995","unstructured":"Krivanek OL, Friedman SL, Gubbens AJ, Kraus B (1995) An imaging filter for biological applications. Ultramicroscopy 59: 267\u2013282","journal-title":"Ultramicroscopy"},{"key":"BFEMBJ2022111158_CR55","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.1091\/mbc.e14-03-0833","volume":"25","author":"S Kumar","year":"2014","unstructured":"Kumar S, Briguglio JS, Turkewitz AP (2014) An aspartyl cathepsin, CTH3, is essential for proprotein processing during secretory granule maturation in Tetrahymena thermophila. Mol Biol Cell 25: 2444\u20132460","journal-title":"Mol Biol Cell"},{"key":"BFEMBJ2022111158_CR56","doi-asserted-by":"crossref","first-page":"4098","DOI":"10.1038\/ncomms5098","volume":"5","author":"MH Lamarque","year":"2014","unstructured":"Lamarque MH, Roques M, Kong\u2010Hap M, Tonkin ML, Rugarabamu G, Marq JB, Penarete\u2010Vargas DM, Boulanger MJ, Soldati\u2010Favre D, Lebrun M (2014) Plasticity and redundancy among AMA\u2010RON pairs ensure host cell entry of Toxoplasma parasites. Nat Commun 5: 4098","journal-title":"Nat Commun"},{"key":"BFEMBJ2022111158_CR57","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/B978-0-12-815041-2.00014-1","volume-title":"Toxoplasma gondii: The Model Apicomplexan; Perspectives and Methods","author":"M Lebrun","year":"2020","unstructured":"Lebrun M, Carrthers VB, Cesbron\u2010Delauw M\u2010F (2020) Toxoplasma secretory proteins and their roles in parasite cell cycle and infection. In Toxoplasma gondii: The Model Apicomplexan; Perspectives and Methods, Weiss LM, Kim K (eds), pp 607\u2013704. Amsterdam: Elsevier"},{"key":"BFEMBJ2022111158_CR58","doi-asserted-by":"crossref","unstructured":"Liebscher I, Cevhero\u011flu O, Hsiao C\u2010C, Maia AF, Schihada H, Scholz N, Soave M, Spiess K, Trajkovi\u0107 K, Kosloff M et\u00a0al (2021) A guide to adhesion GPCR research. FEBS J https:\/\/doi.org\/10.1111\/febs.16258","DOI":"10.1111\/febs.16258"},{"key":"BFEMBJ2022111158_CR59","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0001889","volume":"3","author":"L Madeira","year":"2008","unstructured":"Madeira L, Galante PAF, Budu A, Azevedo MF, Malnic B, Garcia CRS (2008) Genome\u2010wide detection of serpentine receptor\u2010like proteins in malaria parasites. PLoS One 3: e1889","journal-title":"PLoS One"},{"key":"BFEMBJ2022111158_CR60","doi-asserted-by":"crossref","first-page":"4983","DOI":"10.1038\/s41467-021-25309-9","volume":"12","author":"SK Mageswaran","year":"2021","unstructured":"Mageswaran SK, Gu\u00e9rin A, Theveny LM, Chen WD, Martinez M, Lebrun M, Striepen B, Chang Y\u2010W (2021) In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems. Nat Commun 12: 4983","journal-title":"Nat Commun"},{"key":"BFEMBJ2022111158_CR61","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1038\/s41564-022-01171-3","volume":"7","author":"M Martinez","year":"2022","unstructured":"Martinez M, Chen WD, Cova MM, Moln\u00e1r P, Mageswaran SK, Gu\u00e9rin A, John ARO, Lebrun M, Chang Y\u2010W (2022) Rhoptry secretion system structure and priming in plasmodium falciparum revealed using in\u00a0situ cryo\u2010electron tomography. Nat Microbiol 7: 1230\u20131238","journal-title":"Nat Microbiol"},{"key":"BFEMBJ2022111158_CR62","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","volume":"152","author":"DN Mastronarde","year":"2005","unstructured":"Mastronarde DN (2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152: 36\u201351","journal-title":"J Struct Biol"},{"key":"BFEMBJ2022111158_CR63","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1126\/science.1074553","volume":"298","author":"M Meissner","year":"2002","unstructured":"Meissner M, Schluter D, Soldati D (2002) Role of toxoplasma gondii myosin a in powering parasite gliding and host cell invasion. Science 298: 837\u2013840","journal-title":"Science"},{"key":"BFEMBJ2022111158_CR64","doi-asserted-by":"crossref","first-page":"D394","DOI":"10.1093\/nar\/gkaa1106","volume":"49","author":"H Mi","year":"2021","unstructured":"Mi H, Ebert D, Muruganujan A, Mills C, Albou L\u2010P, Mushayamaha T, Thomas PD (2021) PANTHER version 16: a revised family classification, tree\u2010based classification tool, enhancer regions and extensive API. Nucleic Acids Res 49: D394\u2013D403","journal-title":"Nucleic Acids Res"},{"key":"BFEMBJ2022111158_CR65","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.gene.2008.08.007","volume":"425","author":"K Mochizuki","year":"2008","unstructured":"Mochizuki K (2008) High efficiency transformation of Tetrahymena using a codon\u2010optimized neomycin resistance gene. Gene 425: 79\u201383","journal-title":"Gene"},{"key":"BFEMBJ2022111158_CR66","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1550-7408.1996.tb04491.x","volume":"43","author":"R Mondragon","year":"1996","unstructured":"Mondragon R, Frixione E (1996) Ca(2+)\u2010dependence of conoid extrusion in toxoplasma gondii tachyzoites. J Eukaryot Microbiol 43: 120\u2013127","journal-title":"J Eukaryot Microbiol"},{"key":"BFEMBJ2022111158_CR67","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.chom.2013.02.001","volume":"13","author":"C Mueller","year":"2013","unstructured":"Mueller C, Klages N, Jacot D, Santos JM, Cabrera A, Gilberger TW, Dubremetz J\u2010F, Soldati\u2010Favre D (2013) The Toxoplasma protein ARO mediates the apical positioning of rhoptry organelles, a prerequisite for host cell invasion. Cell Host Microbe 13: 289\u2013301","journal-title":"Cell Host Microbe"},{"key":"BFEMBJ2022111158_CR68","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1126\/science.1128618","volume":"313","author":"D Nicastro","year":"2006","unstructured":"Nicastro D, Schwartz C, Pierson J, Gaudette R, Porter ME, McIntosh JR (2006) The molecular architecture of axonemes revealed by cryoelectron tomography. Science 313: 944\u2013948","journal-title":"Science"},{"key":"BFEMBJ2022111158_CR69","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0022-5320(83)90067-9","volume":"83","author":"BA Nichols","year":"1983","unstructured":"Nichols BA, Chiappino ML, O'Connor GR (1983) Secretion from the rhoptries of Toxoplasma gondii during host\u2010cell invasion. J Ultrastruct Res 83: 85\u201398","journal-title":"J Ultrastruct Res"},{"key":"BFEMBJ2022111158_CR70","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/nmeth.1401","volume":"6","author":"K Nishimura","year":"2009","unstructured":"Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M (2009) An auxin\u2010based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6: 917\u2013922","journal-title":"Nat Methods"},{"key":"BFEMBJ2022111158_CR71","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0014-5793(84)80473-1","volume":"171","author":"L Patthy","year":"1984","unstructured":"Patthy L, Trexler M, V\u00e1li Z, B\u00e1nyai L, V\u00e1radi A (1984) Kringles: modules specialized for protein binding. Homology of the gelatin\u2010binding region of fibronectin with the kringle structures of proteases. FEBS Lett 171: 131\u2013136","journal-title":"FEBS Lett"},{"key":"BFEMBJ2022111158_CR72","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gky1106","volume":"47","author":"Y Perez\u2010Riverol","year":"2019","unstructured":"Perez\u2010Riverol Y, Csordas A, Bai J, Bernal\u2010Llinares M, Hewapathirana S, Kundu DJ, Inuganti A, Griss J, Mayer G, Eisenacher M et\u00a0al (2019) The PRIDE database and related tools and resources in 2019: improving support for quantification data. Nucleic Acids Res 47: D442\u2013D450","journal-title":"Nucleic Acids Res"},{"key":"BFEMBJ2022111158_CR73","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.ijbiomac.2020.02.320","volume":"152","author":"AJ Pietrzyk\u2010Brzezinska","year":"2020","unstructured":"Pietrzyk\u2010Brzezinska AJ, Bujacz A (2020) H\u2010type lectins \u2013 structural characteristics and their applications in diagnostics, analytics and drug delivery. Int J Biol Macromol 152: 735\u2013747","journal-title":"Int J Biol Macromol"},{"key":"BFEMBJ2022111158_CR74","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/978-1-4939-9857-9_6","volume":"2071","author":"F Piro","year":"2020","unstructured":"Piro F, Carruthers VB, Di Cristina M (2020) PCR screening of Toxoplasma gondii single clones directly from 96\u2010well plates without DNA purification. Methods Mol Biol 2071: 117\u2013123","journal-title":"Methods Mol Biol"},{"key":"BFEMBJ2022111158_CR75","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1242\/jcs.13.3.687","volume":"13","author":"H Plattner","year":"1973","unstructured":"Plattner H, Miller F, Bachmann L (1973) Membrane specializations in the form of regular membrane\u2010to\u2010membrane attachment sites in Paramecium. A correlated freeze\u2010etching and ultrathin\u2010sectioning analysis. J Cell Sci 13: 687\u2013719","journal-title":"J Cell Sci"},{"key":"BFEMBJ2022111158_CR76","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.899243","volume":"13","author":"A Possenti","year":"2022","unstructured":"Possenti A, Di Cristina M, Nicastro C, Lunghi M, Messina V, Piro F, Tramontana L, Cherchi S, Falchi M, Bertuccini L et\u00a0al (2022) Functional characterization of the thrombospondin\u2010related paralogous proteins rhoptry discharge factors 1 and 2 unveils phenotypic plasticity in toxoplasma gondii rhoptry exocytosis. Front Microbiol 13: 899243","journal-title":"Front Microbiol"},{"key":"BFEMBJ2022111158_CR77","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.chom.2010.12.003","volume":"9","author":"DT Riglar","year":"2011","unstructured":"Riglar DT, Richard D, Wilson DW, Boyle MJ, Dekiwadia C, Turnbull L, Angrisano F, Marapana DS, Rogers KL, Whitchurch CB et\u00a0al (2011) Super\u2010resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte. Cell Host Microbe 9: 9\u201320","journal-title":"Cell Host Microbe"},{"key":"BFEMBJ2022111158_CR78","doi-asserted-by":"crossref","first-page":"8712","DOI":"10.1074\/jbc.M113.450932","volume":"288","author":"MS Roiko","year":"2013","unstructured":"Roiko MS, Carruthers VB (2013) Functional dissection of Toxoplasma gondii perforin\u2010like protein 1 reveals a dual domain mode of membrane binding for cytolysis and parasite egress. J Biol Chem 288: 8712\u20138725","journal-title":"J Biol Chem"},{"key":"BFEMBJ2022111158_CR79","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0309-1651(77)90012-1","volume":"1","author":"B Satir","year":"1977","unstructured":"Satir B (1977) Dibucaine\u2010induced synchronous mucocyst secretion in Tetrahymena. Cell Biol Int Rep 1: 69\u201373","journal-title":"Cell Biol Int Rep"},{"key":"BFEMBJ2022111158_CR80","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/235053a0","volume":"235","author":"B Satir","year":"1972","unstructured":"Satir B, Schooley C, Satir P (1972) Membrane reorganization during secretion in Tetrahymena. Nature 235: 53\u201354","journal-title":"Nature"},{"key":"BFEMBJ2022111158_CR81","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda\u2010Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B et\u00a0al (2012) Fiji: an open\u2010source platform for biological\u2010image analysis. Nat Methods 9: 676\u2013682","journal-title":"Nat Methods"},{"key":"BFEMBJ2022111158_CR82","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25\u2009years of image analysis. Nat Methods 9: 671\u2013675","journal-title":"Nat Methods"},{"key":"BFEMBJ2022111158_CR83","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002392","volume":"7","author":"L Sheiner","year":"2011","unstructured":"Sheiner L, Demerly JL, Poulsen N, Beatty WL, Lucas O, Behnke MS, White MW, Striepen B (2011) A systematic screen to discover and analyze apicoplast proteins identifies a conserved and essential protein import factor. PLoS Pathog 7: e1002392","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR84","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","volume":"166","author":"SM Sidik","year":"2016","unstructured":"Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC et\u00a0al (2016) A genome\u2010wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes. Cell 166: 1423\u20131435.e12","journal-title":"Cell"},{"key":"BFEMBJ2022111158_CR85","doi-asserted-by":"crossref","unstructured":"Singer M, Simon K, Forn\u00e9 I, Meissner M (2022) A central protein complex essential for invasion in Toxoplasma gondii. bioRxiv https:\/\/doi.org\/10.1101\/2022.02.24.481622 [PREPRINT]","DOI":"10.1101\/2022.02.24.481622"},{"key":"BFEMBJ2022111158_CR86","doi-asserted-by":"crossref","DOI":"10.1371\/annotation\/1ccae8f8-d274-4ff8-a295-815037ce9cc6","volume":"6","author":"S Singh","year":"2010","unstructured":"Singh S, Alam MM, Pal\u2010Bhowmick I, Brzostowski JA, Chitnis CE (2010) Distinct external signals trigger sequential release of apical organelles during erythrocyte invasion by malaria parasites. PLoS Pathog 6: e1000746","journal-title":"PLoS Pathog"},{"key":"BFEMBJ2022111158_CR87","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.jprot.2013.07.028","volume":"91","author":"A Skorupa","year":"2013","unstructured":"Skorupa A, Urbach S, Vigy O, King MA, Chaumont\u2010Dubel S, Prehn JH, Marin P (2013) Angiogenin induces modifications in the astrocyte secretome: relevance to amyotrophic lateral sclerosis. J Proteomics 91: 274\u2013285","journal-title":"J Proteomics"},{"key":"BFEMBJ2022111158_CR88","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.pt.2021.04.011","volume":"37","author":"D Sparvoli","year":"2021","unstructured":"Sparvoli D, Lebrun M (2021) Unraveling the elusive rhoptry exocytic mechanism of Apicomplexa. Trends Parasitol 37: 622\u2013637","journal-title":"Trends Parasitol"},{"key":"BFEMBJ2022111158_CR89","doi-asserted-by":"crossref","first-page":"jcs238659","DOI":"10.1242\/jcs.238659","volume":"133","author":"D Sparvoli","year":"2020","unstructured":"Sparvoli D, Zoltner M, Cheng C\u2010Y, Field MC, Turkewitz AP (2020) Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila. J Cell Sci 133: jcs238659","journal-title":"J Cell Sci"},{"key":"BFEMBJ2022111158_CR90","doi-asserted-by":"crossref","first-page":"4041","DOI":"10.1038\/s41467-019-11979-z","volume":"10","author":"C Suarez","year":"2019","unstructured":"Suarez C, Lentini G, Ramaswamy R, Maynadier M, Aquilini E, Berry\u2010Sterkers L, Cipriano M, Chen AL, Bradley P, Striepen B et\u00a0al (2019) A lipid\u2010binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites. Nat Commun 10: 4041","journal-title":"Nat Commun"},{"key":"BFEMBJ2022111158_CR91","doi-asserted-by":"crossref","first-page":"8413","DOI":"10.1073\/pnas.93.16.8413","volume":"93","author":"E Suss\u2010Toby","year":"1996","unstructured":"Suss\u2010Toby E, Zimmerberg J, Ward GE (1996) Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc Natl Acad Sci U S A 93: 8413\u20138418","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BFEMBJ2022111158_CR92","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","volume":"38","author":"K Tamura","year":"2021","unstructured":"Tamura K, Stecher G, Kumar S (2021) MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38: 3022\u20133027","journal-title":"Mol Biol Evol"},{"key":"BFEMBJ2022111158_CR93","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1111\/j.1462-5822.2006.00885.x","volume":"9","author":"J Thompson","year":"2007","unstructured":"Thompson J, Fernandez\u2010Reyes D, Sharling L, Moore SG, Eling WM, Kyes SA, Newbold CI, Kafatos FC, Janse CJ, Waters AP (2007) Plasmodium cysteine repeat modular proteins 1\u20104: Complex proteins with roles throughout the malaria parasite life cycle. Cell Microbiol 9: 1466\u20131480","journal-title":"Cell Microbiol"},{"key":"BFEMBJ2022111158_CR94","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.softx.2017.06.006","volume":"6","author":"LM Tsypin","year":"2017","unstructured":"Tsypin LM, Turkewitz AP (2017) The Co\u2010regulation data harvester: automating gene annotation starting from a transcriptome database. SoftwareX 6: 165\u2013171","journal-title":"SoftwareX"},{"key":"BFEMBJ2022111158_CR95","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1002\/j.1460-2075.1991.tb07727.x","volume":"10","author":"AP Turkewitz","year":"1991","unstructured":"Turkewitz AP, Madeddu L, Kelly RB (1991) Maturation of dense core granules in wild type and mutant Tetrahymena thermophila. EMBO J 10: 1979\u20131987","journal-title":"EMBO J"},{"key":"BFEMBJ2022111158_CR96","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","volume":"13","author":"S Tyanova","year":"2016","unstructured":"Tyanova S, Temu T, Sinitcyn P, Carlson A, Hein MY, Geiger T, Mann M, Cox J (2016) The perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods 13: 731\u2013740","journal-title":"Nat Methods"},{"key":"BFEMBJ2022111158_CR97","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.tibs.2008.07.005","volume":"33","author":"S Yona","year":"2008","unstructured":"Yona S, Lin H\u2010H, Siu WO, Gordon S, Stacey M (2008) Adhesion\u2010GPCRs: emerging roles for novel receptors. Trends Biochem Sci 33: 491\u2013500","journal-title":"Trends Biochem Sci"},{"key":"BFEMBJ2022111158_CR98","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.jmb.2017.12.007","volume":"430","author":"L Zimmermann","year":"2018","unstructured":"Zimmermann L, Stephens A, Nam S\u2010Z, Rau D, K\u00fcbler J, Lozajic M, Gabler F, S\u00f6ding J, Lupas AN, Alva V (2018) A completely reimplemented MPI bioinformatics toolkit with a new HHpred server at its core. J Mol Biol 430: 2237\u20132243","journal-title":"J Mol Biol"}],"container-title":["The EMBO Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embj.2022111158.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.15252\/embj.2022111158","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/embj.2022111158\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/embj.2022111158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embj.2022111158.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T20:35:53Z","timestamp":1779309353000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/embj.2022111158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,17]]},"references-count":98,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2022,11,17]]}},"alternative-id":["BFEMBJ2022111158"],"URL":"https:\/\/doi.org\/10.15252\/embj.2022111158","relation":{},"ISSN":["0261-4189","1460-2075"],"issn-type":[{"value":"0261-4189","type":"print"},{"value":"1460-2075","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,17]]},"assertion":[{"value":"14 March 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 September 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 September 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 October 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"EMBJ2022111158"}}