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The events that drive rhoptry discharge are poorly understood, yet essential to sustain the apicomplexan parasitic life cycle. Rhoptry discharge appears to depend on proteins secreted from another set of organelles called micronemes, which vary in function from allowing host cell binding to facilitation of gliding motility. Here we examine the function of the microneme protein CLAMP, which we previously found to be necessary for\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    host cell invasion, and demonstrate its essential role in rhoptry discharge. CLAMP forms a distinct complex with two other microneme proteins, the invasion\u2010associated SPATR, and a previously uncharacterized protein we name CLAMP\u2010linked invasion protein (CLIP). CLAMP deficiency does not impact parasite adhesion or microneme protein secretion; however, knockdown of any member of the CLAMP complex affects rhoptry discharge. Phylogenetic analysis suggests orthologs of the essential complex components, CLAMP and CLIP, are ubiquitous across apicomplexans. SPATR appears to act as an accessory factor in\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    , but despite incomplete conservation is also essential for invasion during\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    blood stages. Together, our results reveal a new protein complex that mediates rhoptry discharge following host\u2010cell contact.\n                  <\/jats:p>","DOI":"10.15252\/embj.2022113155","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T05:33:17Z","timestamp":1698384797000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["A conserved complex of microneme proteins mediates rhoptry discharge in Toxoplasma"],"prefix":"10.1038","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-9067","authenticated-orcid":false,"given":"Dylan","family":"Valleau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima M","family":"Sidik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6981-377X","authenticated-orcid":false,"given":"Luiz C","family":"Godoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamilex","family":"Acevedo\u2010S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9780-3680","authenticated-orcid":false,"given":"Charisse Flerida A","family":"Pasaje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"My\u2010Hang","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vern B","family":"Carruthers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6250-8796","authenticated-orcid":false,"given":"Jacquin C","family":"Niles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5237-1095","authenticated-orcid":false,"given":"Sebastian","family":"Lourido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"key":"BFEMBJ2022113155_CR1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0166-6851(91)90182-6","volume":"47","author":"A Achbarou","year":"1991","unstructured":"Achbarou A, Mercereau\u2010Puijalon O, Autheman JM, Fortier B, Camus D, Dubremetz JF (1991) Characterization of microneme proteins of Toxoplasma gondii. 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