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STAT1 is activated by Janus kinase (JAK)\u2010mediated phosphorylation of Y701. To determine whether STAT1 contributes to cellular responses without this phosphorylation event, we generated mice with Y701 mutated to a phenylalanine (Stat1\n                    <jats:sup>Y701F<\/jats:sup>\n                    ). We show that heterozygous mice do not exhibit a dominant\u2010negative phenotype. Homozygous Stat1\n                    <jats:sup>Y701F<\/jats:sup>\n                    mice show a profound reduction in Stat1 expression, highlighting an important role for basal IFN\u2010dependent signaling. The rapid transcriptional response to type I IFN (IFN\u2010I) and type II IFN (IFN\u03b3) was absent in Stat1\n                    <jats:sup>Y701F<\/jats:sup>\n                    cells. Intriguingly, STAT1Y701F suppresses the delayed expression of IFN\u2010I\u2010stimulated genes (ISG) observed in Stat1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    cells, mediated by the STAT2\/IRF9 complex. Thus, Stat1\n                    <jats:sup>Y701F<\/jats:sup>\n                    macrophages are more susceptible to\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    infection than Stat1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    macrophages.\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    grew less robustly in Stat1\n                    <jats:sup>Y701F<\/jats:sup>\n                    macrophages and mice compared to Stat1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    counterparts, but STAT1Y701F is not sufficient to rescue the animals. Our studies are consistent with a potential contribution of Y701\u2010unphosphorylated STAT1 to innate antibacterial immunity.\n                  <\/jats:p>","DOI":"10.15252\/embr.201540726","type":"journal-article","created":{"date-parts":[[2016,2,13]],"date-time":"2016-02-13T06:32:39Z","timestamp":1455345159000},"page":"367-382","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Response to interferons and antibacterial innate immunity in the absence of tyrosine\u2010phosphorylated STAT1"],"prefix":"10.1038","volume":"17","author":[{"given":"Andrea","family":"Majoros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterini","family":"Platanitis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Szappanos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HyeonJoo","family":"Cheon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Vogl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyank","family":"Shukla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George R","family":"Stark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Sexl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Schindler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Decker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,12]]},"reference":[{"key":"BFEMBR201540726_CR1","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1038\/nrd2422","volume":"6","author":"EC Borden","year":"2007","unstructured":"Borden EC, Sen GC, Uze G, Silverman RH, Ransohoff RM, Foster GR, Stark GR (2007) Interferons at age 50: past, current and future impact on biomedicine. 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