{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:28:20Z","timestamp":1779114500985,"version":"3.51.4"},"reference-count":79,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T00:00:00Z","timestamp":1560816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T00:00:00Z","timestamp":1560816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["IG\/15\/1\/31328"],"award-info":[{"award-number":["IG\/15\/1\/31328"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHU 1228\/2\u20102"],"award-info":[{"award-number":["SCHU 1228\/2\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["210152\/Z\/18\/Z"],"award-info":[{"award-number":["210152\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Sheffield Node Studentship awarded from the Infection, Immunity, and Cardiovascular Disease Department, University of Sheffield"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Rep"],"published-print":{"date-parts":[[2019,8,1]]},"DOI":"10.15252\/embr.201847047","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T12:45:17Z","timestamp":1560861917000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cerebrovascular endothelial cells form transient Notch\u2010dependent cystic structures in zebrafish"],"prefix":"10.1038","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2536-6140","authenticated-orcid":false,"given":"Elisabeth C","family":"Kugler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max","family":"van Lessen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Daetwyler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karishma","family":"Chhabria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron M","family":"Savage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishmi","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Plant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan B","family":"MacDonald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Huisken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert N","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Schulte\u2010Merker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Armitage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7458-5481","authenticated-orcid":false,"given":"Timothy JA","family":"Chico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,6,18]]},"reference":[{"key":"BFEMBR201847047_CR1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/nrm.2017.36","volume":"18","author":"M Potente","year":"2017","unstructured":"Potente M, M\u00e4kinen T (2017) Vascular heterogeneity and specialization in development and disease. Nat Rev Mol Cell Biol 18: 477\u2013494","journal-title":"Nat Rev Mol Cell Biol"},{"key":"BFEMBR201847047_CR2","volume-title":"Angiogenesis","author":"TH Adair","year":"2010","unstructured":"Adair TH, Montani JP (2010) Angiogenesis. San Rafael, CA: Morgan & Claypool Life Sciences"},{"key":"BFEMBR201847047_CR3","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/B978-0-12-386041-5.00003-0","volume":"288","author":"GE Davis","year":"2011","unstructured":"Davis GE, Stratman AN, Sacharidou A, Koh W (2011) Molecular basis for endothelial lumen formation and tubulogenesis during vasculogenesis and angiogenic sprouting. Int Rev Cell Mol Biol 288: 101\u2013165","journal-title":"Int Rev Cell Mol Biol"},{"key":"BFEMBR201847047_CR4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0014-5793(96)01289-6","volume":"399","author":"S Dimmeler","year":"1996","unstructured":"Dimmeler S, Haendeler J, Rippmann V, Nehls M, Zeiher AM (1996) Shear stress inhibits apoptosis of human endothelial cells. FEBS Lett 399: 71\u201374","journal-title":"FEBS Lett"},{"key":"BFEMBR201847047_CR5","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1152\/physrev.00038.2016","volume":"97","author":"P Gut","year":"2017","unstructured":"Gut P, Reischauer S, Stainier DYR, Arnaout R (2017) Little fish, big data: zebrafish as a model for cardiovascular and metabolic disease. Physiol Rev 97: 889\u2013938","journal-title":"Physiol Rev"},{"key":"BFEMBR201847047_CR6","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.tcm.2008.04.002","volume":"18","author":"TJA Chico","year":"2008","unstructured":"Chico TJA, Ingham PW, Crossman DC (2008) Modeling cardiovascular disease in the zebrafish. Trends Cardiovasc Med 18: 150\u2013155","journal-title":"Trends Cardiovasc Med"},{"key":"BFEMBR201847047_CR7","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1126\/science.1100035","volume":"305","author":"J Huisken","year":"2004","unstructured":"Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK (2004) Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305: 1007\u20131009","journal-title":"Science"},{"key":"BFEMBR201847047_CR8","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1006\/dbio.2002.0711","volume":"248","author":"ND Lawson","year":"2002","unstructured":"Lawson ND, Weinstein BM (2002) In vivo imaging of embryonic vascular development using transgenic zebrafish. Dev Biol 248: 307\u2013318","journal-title":"Dev Biol"},{"key":"BFEMBR201847047_CR9","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1007\/s00018-018-2983-9","volume":"76","author":"S Paone","year":"2018","unstructured":"Paone S, Baxter AA, Hulett MD, Poon IKH (2018) Endothelial cell apoptosis and the role of endothelial cell\u2010derived extracellular vesicles in the progression of atherosclerosis. Cell Mol Life Sci 76: 1093\u20131106","journal-title":"Cell Mol Life Sci"},{"key":"BFEMBR201847047_CR10","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-cellbio-101512-122326","volume":"30","author":"M Colombo","year":"2014","unstructured":"Colombo M, Raposo G, Th\u00e9ry C (2014) Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol 30: 255\u2013289","journal-title":"Annu Rev Cell Dev Biol"},{"key":"BFEMBR201847047_CR11","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.tcm.2018.10.010","volume":"29","author":"P Zamani","year":"2018","unstructured":"Zamani P, Fereydouni N, Butler AE, Navashenaq JG, Sahebkar A (2018) The therapeutic and diagnostic role of exosomes in cardiovascular diseases. Trends Cardiovasc Med 29: 313\u2013323","journal-title":"Trends Cardiovasc Med"},{"key":"BFEMBR201847047_CR12","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1016\/S0002-8703(03)00103-0","volume":"145","author":"L Bernal\u2010Mizrachi","year":"2003","unstructured":"Bernal\u2010Mizrachi L, Jy W, Jimenez JJ, Pastor J, Mauro LM, Horstman LL, de Marchena E, Ahn YS (2003) High levels of circulating endothelial microparticles in patients with acute coronary syndromes. Am Heart J 145: 962\u2013970","journal-title":"Am Heart J"},{"key":"BFEMBR201847047_CR13","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1529\/biophysj.107.113605","volume":"94","author":"GT Charras","year":"2008","unstructured":"Charras GT, Coughlin M, Mitchison TJ, Mahadevan L (2008) Life and times of a cellular bleb. Biophys J 94: 1836\u20131853","journal-title":"Biophys J"},{"key":"BFEMBR201847047_CR14","doi-asserted-by":"crossref","first-page":"e25932","DOI":"10.7554\/eLife.25932","volume":"6","author":"M van Lessen","year":"2017","unstructured":"van Lessen M, Shibata\u2010Germanos S, van Impel A, Hawkins TA, Rihel J, Schulte\u2010Merker S (2017) Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development. Elife 6: e25932","journal-title":"Elife"},{"key":"BFEMBR201847047_CR15","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.devcel.2017.08.015","volume":"42","author":"BM Hogan","year":"2017","unstructured":"Hogan BM, Schulte\u2010Merker S (2017) How to plumb a pisces: understanding vascular development and disease using zebrafish embryos. Dev Cell 42: 567\u2013583","journal-title":"Dev Cell"},{"key":"BFEMBR201847047_CR16","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1161\/CIRCULATIONAHA.114.015036","volume":"132","author":"EO Agbani","year":"2015","unstructured":"Agbani EO, van den Bosch MTJ, Brown E, Williams CM, Mattheij NJA, Cosemans JMEM, Collins PW, Heemskerk JWM, Hers I, Poole AW (2015) Coordinated membrane ballooning and procoagulant spreading in human platelets clinical perspective. Circulation 132: 1414\u20131424","journal-title":"Circulation"},{"key":"BFEMBR201847047_CR17","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1083\/jcb.200302047","volume":"161","author":"H Gerhardt","year":"2003","unstructured":"Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D et\u00a0al (2003) VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J Cell Biol 161: 1163\u20131177","journal-title":"J Cell Biol"},{"key":"BFEMBR201847047_CR18","first-page":"1","volume":"2","author":"AV Gore","year":"2012","unstructured":"Gore AV, Monzo K, Cha YR, Pan W, Weinstein BM (2012) Vascular development in the zebrafish. Cold Spring Harb Perspect Med 2: 1\u201322","journal-title":"Cold Spring Harb Perspect Med"},{"key":"BFEMBR201847047_CR19","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1038\/nature05577","volume":"445","author":"AF Siekmann","year":"2007","unstructured":"Siekmann AF, Lawson ND (2007) Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries. Nature 445: 781\u2013784","journal-title":"Nature"},{"key":"BFEMBR201847047_CR20","doi-asserted-by":"crossref","first-page":"558","DOI":"10.2174\/138945008784911822","volume":"9","author":"NL Parmalee","year":"2008","unstructured":"Parmalee NL, Kitajewski J (2008) Wnt signaling in angiogenesis. Curr Drug Targets 9: 558\u2013564","journal-title":"Curr Drug Targets"},{"key":"BFEMBR201847047_CR21","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/ng.321","volume":"41","author":"BM Hogan","year":"2009","unstructured":"Hogan BM, Bos FL, Bussmann J, Witte M, Chi NC, Duckers HJ, Schulte\u2010Merker S (2009) Ccbe1 is required for embryonic lymphangiogenesis and venous sprouting. Nat Genet 41: 396\u2013398","journal-title":"Nat Genet"},{"key":"BFEMBR201847047_CR22","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1038\/ncb3320","volume":"18","author":"V Gebala","year":"2016","unstructured":"Gebala V, Collins R, Geudens I, Phng LK, Gerhardt H (2016) Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in\u00a0vivo. Nat Cell Biol 18: 443\u2013450","journal-title":"Nat Cell Biol"},{"key":"BFEMBR201847047_CR23","first-page":"497","volume":"142","author":"T Kashiwada","year":"2015","unstructured":"Kashiwada T, Fukuhara S, Terai K, Tanaka T, Wakayama Y, Ando K, Nakajima H, Fukui H, Yuge S, Saito Y et\u00a0al (2015) beta\u2010catenin\u2010dependent transcription is central to Bmp\u2010mediated formation of venous vessels. Development 142: 497\u2013509","journal-title":"Development"},{"key":"BFEMBR201847047_CR24","doi-asserted-by":"crossref","first-page":"4081","DOI":"10.1242\/dev.091702","volume":"140","author":"H\u2010B Kwon","year":"2013","unstructured":"Kwon H\u2010B, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K, Hibi M, Kwon Y\u2010G, Kim K\u2010W, Alitalo K et\u00a0al (2013) The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc\/Vegfr3 signaling in zebrafish. Development 140: 4081\u20134090","journal-title":"Development"},{"key":"BFEMBR201847047_CR25","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/ng875","volume":"31","author":"AJ Sehnert","year":"2002","unstructured":"Sehnert AJ, Huq A, Weinstein BM, Walker C, Fishman M, Stainier DY (2002) Cardiac troponin T is essential in sarcomere assembly and cardiac contractility. Nat Genet 31: 106\u2013110","journal-title":"Nat Genet"},{"key":"BFEMBR201847047_CR26","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1152\/physiolgenomics.00049.2009","volume":"38","author":"IM Packham","year":"2009","unstructured":"Packham IM, Gray C, Heath PR, Hellewell PG, Ingham PW, Crossman DC, Milo M, Chico TJ (2009) Microarray profiling reveals CXCR26a is downregulated by blood flow in\u00a0vivo and mediates collateral formation in zebrafish embryos. Physiol Genomics 38: 319\u2013327","journal-title":"Physiol Genomics"},{"key":"BFEMBR201847047_CR27","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/cvr\/cvt170","volume":"100","author":"O Watson","year":"2013","unstructured":"Watson O, Novodvorsky P, Gray C, Rothman AM, Lawrie A, Crossman DC, Haase A, McMahon K, Gering M, Van Eeden FJ et\u00a0al (2013) Blood flow suppresses vascular Notch signalling via dll4 and is required for angiogenesis in response to hypoxic signalling. Cardiovasc Res 100: 252\u2013261","journal-title":"Cardiovasc Res"},{"key":"BFEMBR201847047_CR28","doi-asserted-by":"crossref","first-page":"13982","DOI":"10.1021\/ja063363t","volume":"128","author":"R Notman","year":"2006","unstructured":"Notman R, Noro M, O'Malley B, Anwar J (2006) Molecular basis for dimethylsulfoxide (DMSO) action on lipid membranes. J Am Chem Soc 128: 13982\u201313983","journal-title":"J Am Chem Soc"},{"key":"BFEMBR201847047_CR29","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1182\/blood-2009-12-257832","volume":"116","author":"A Fantin","year":"2010","unstructured":"Fantin A, Vieira JM, Gestri G, Denti L, Schwarz Q, Prykhozhij S, Peri F, Wilson SW, Ruhrberg C (2010) Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF\u2010mediated endothelial tip cell induction. Blood 116: 829\u2013840","journal-title":"Blood"},{"key":"BFEMBR201847047_CR30","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1038\/nature07083","volume":"454","author":"T Tammela","year":"2008","unstructured":"Tammela T, Zarkada G, Wallgard E, Murtom\u00e4ki A, Suchting S, Wirzenius M, Waltari M, Hellstr\u00f6m M, Schomber T, Peltonen R et\u00a0al (2008) Blocking VEGFR\u20103 suppresses angiogenic sprouting and vascular network formation. Nature 454: 656\u2013660","journal-title":"Nature"},{"key":"BFEMBR201847047_CR31","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1172\/JCI119451","volume":"99","author":"M Ziche","year":"1997","unstructured":"Ziche M, Morbidelli L, Choudhuri R, Zhang HT, Donnini S, Granger HJ, Bicknell R (1997) Nitric oxide synthase lies downstream from vascular endothelial growth factor\u2010induced but not basic fibroblast growth factor\u2010induced angiogenesis. J Clin Invest 99: 2625\u20132634","journal-title":"J Clin Invest"},{"key":"BFEMBR201847047_CR32","doi-asserted-by":"crossref","first-page":"2446","DOI":"10.1021\/ac9801723","volume":"70","author":"H Kojima","year":"1998","unstructured":"Kojima H, Nakatsubo N, Kikuchi K, Kawahara S, Kirino Y, Nagoshi H, Hirata Y, Nagano T (1998) Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins. Anal Chem 70: 2446\u20132453","journal-title":"Anal Chem"},{"key":"BFEMBR201847047_CR33","doi-asserted-by":"crossref","first-page":"3209","DOI":"10.1002\/(SICI)1521-3773(19991102)38:21<3209::AID-ANIE3209>3.0.CO;2-6","volume":"38","author":"H Kojima","year":"1999","unstructured":"Kojima H, Urano Y, Kikuchi K, Higuchi T, Hirata Y, Nagano T (1999) Fluorescent indicators for imaging nitric oxide production. Angew Chem Int Ed Engl 38: 3209\u20133212","journal-title":"Angew Chem Int Ed Engl"},{"key":"BFEMBR201847047_CR34","doi-asserted-by":"crossref","first-page":"48472","DOI":"10.1074\/jbc.M209130200","volume":"277","author":"X Zhang","year":"2002","unstructured":"Zhang X, Kim WS, Hatcher N, Potgieter K, Moroz LL, Gillette R, Sweedler JV (2002) Interfering with nitric oxide measurements 4,5\u2010diaminofluorescein reactd with dehydroascorbic acid and ascorbic acid. J Biol Chem 277: 48472\u201348478","journal-title":"J Biol Chem"},{"key":"BFEMBR201847047_CR35","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0143-4160(96)90084-6","volume":"19","author":"T Haller","year":"1996","unstructured":"Haller T, Dietl P, Deetjen P, V\u00f6lkl H (1996) The lysosomal compartment as intracellular calcium store in MDCK cells: a possible involvement in InsP3\u2010mediated Ca2+ release. Cell Calcium 19: 157\u2013165","journal-title":"Cell Calcium"},{"key":"BFEMBR201847047_CR36","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/(SICI)1097-0320(19981101)33:3<348::AID-CYTO9>3.0.CO;2-C","volume":"33","author":"RM Zucker","year":"1998","unstructured":"Zucker RM, Hunter S, Rogers JM (1998) Confocal laser scanning microscopy of apoptosis in organogenesis\u2010stage mouse embryos. Cytometry 33: 348\u2013354","journal-title":"Cytometry"},{"key":"BFEMBR201847047_CR37","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1161\/ATVBAHA.107.143990","volume":"27","author":"C Gray","year":"2007","unstructured":"Gray C, Packham IM, Wurmser F, Eastley NC, Hellewell PG, Ingham PW, Crossman DC, Chico TJ (2007) Ischemia is not required for arteriogenesis in zebrafish embryos. Arterioscler Thromb Vasc Biol 27: 2135\u20132141","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"BFEMBR201847047_CR38","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1007\/s00018-013-1312-6","volume":"70","author":"JL Thomas","year":"2013","unstructured":"Thomas JL, Baker K, Han J, Calvo C, Nurmi H, Eichmann AC, Alitalo K (2013) Interactions between VEGFR and Notch signaling pathways in endothelial and neural cells. Cell Mol Life Sci 70: 1779\u20131792","journal-title":"Cell Mol Life Sci"},{"key":"BFEMBR201847047_CR39","doi-asserted-by":"crossref","first-page":"e1000549","DOI":"10.1371\/journal.pcbi.1000549","volume":"5","author":"K Bentley","year":"2009","unstructured":"Bentley K, Mariggi G, Gerhardt H, Bates PA (2009) Tipping the balance: robustness of tip cell selection, migration and fusion in angiogenesis. PLoS Comput Biol 5: e1000549","journal-title":"PLoS Comput Biol"},{"key":"BFEMBR201847047_CR40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7554\/eLife.06489","volume":"4","author":"B Vanhollebeke","year":"2015","unstructured":"Vanhollebeke B, Stone OA, Bostaille N, Cho C, Zhou Y, Maquet E, Gauquier A, Cabochette P, Fukuhara S, Mochizuki N et\u00a0al (2015) Tip cell\u2010specific requirement for an atypical Gpr124\u2010 and Reck\u2010dependent Wnt\/beta\u2010catenin pathway during brain angiogenesis. Elife 4: 1\u201325","journal-title":"Elife"},{"key":"BFEMBR201847047_CR41","doi-asserted-by":"crossref","first-page":"4860","DOI":"10.1038\/s41467-018-07302-x","volume":"9","author":"K H\u00fcbner","year":"2018","unstructured":"H\u00fcbner K, Cabochette P, Di\u00e9guez\u2010Hurtado R, Wiesner C, Wakayama Y, Grassme KS, Hubert M, Guenther S, Belting HG, Affolter M et\u00a0al (2018) Wnt\/beta\u2010catenin signaling regulates VE\u2010cadherin\u2010mediated anastomosis of brain capillaries by counteracting S1pr1 signaling. Nat Commun 9: 4860","journal-title":"Nat Commun"},{"key":"BFEMBR201847047_CR42","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/nature08356","volume":"461","author":"SM Huang","year":"2009","unstructured":"Huang SM, Mishina YM, Liu S, Cheung A, Stegmeier F, Michaud GA, Charlat O, Wiellette E, Zhang Y, Wiessner S et\u00a0al (2009) Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461: 614\u2013620","journal-title":"Nature"},{"key":"BFEMBR201847047_CR43","doi-asserted-by":"crossref","first-page":"11573","DOI":"10.1073\/pnas.0913138107","volume":"107","author":"J Du","year":"2010","unstructured":"Du J, Wei Y, Liu L, Wang Y, Khairova R, Blumenthal R, Tragon T, Hunsberger JG, Machado\u2010Vieira R, Drevets W et\u00a0al (2010) A kinesin signaling complex mediates the ability of GSK\u20103beta to affect mood\u2010associated behaviors. Proc Natl Acad Sci USA 107: 11573\u201311578","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBR201847047_CR44","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1182\/blood-2002-01-0046","volume":"100","author":"RJ Dekker","year":"2002","unstructured":"Dekker RJ, van Soest S, Fontijn RD, Salamanca S, de Groot PG, VanBavel E, Pannekoek H, Horrevoets AJ (2002) Prolonged fluid shear stress induces a distinct set of endothelial cell genes, most specifically lung Kr\u00fcppel\u2010like factor (KLF2). Blood 100: 1689\u20131698","journal-title":"Blood"},{"key":"BFEMBR201847047_CR45","doi-asserted-by":"crossref","first-page":"11893","DOI":"10.1073\/pnas.1300805110","volume":"110","author":"N Sacilotto","year":"2013","unstructured":"Sacilotto N, Monteiro R, Fritzsche M, Becker PW, Sanchez\u2010Del\u2010Campo L, Liu K, Pinheiro P, Ratnayaka I, Davies B, Goding CR et\u00a0al (2013) Analysis of Dll4 regulation reveals a combinatorial role for Sox and Notch in arterial development. Proc Natl Acad Sci USA 110: 11893\u201311898","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBR201847047_CR46","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.devcel.2014.11.018","volume":"32","author":"FO Kok","year":"2015","unstructured":"Kok FO, Shin M, Ni CW, Gupta A, Grosse AS, van Impel A, Kirchmaier BC, Peterson\u2010Maduro J, Kourkoulis G, Male I et\u00a0al (2015) Reverse genetic screening reveals poor correlation between Morpholino\u2010induced and mutant phenotypes in zebrafish. Dev Cell 32: 97\u2013108","journal-title":"Dev Cell"},{"key":"BFEMBR201847047_CR47","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/s41467-019-08590-7","volume":"10","author":"AM Savage","year":"2019","unstructured":"Savage AM, Kurusamy S, Chen Y, Jiang Z, Chhabria K, MacDonald RB, Kim HR, Wilson HL, van Eeden FJM, Armesilla AL et\u00a0al (2019) tmem33 is essential for VEGF\u2010mediated endothelial calcium oscillations and angiogenesis. Nat Commun 10: 732","journal-title":"Nat Commun"},{"key":"BFEMBR201847047_CR48","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.redox.2015.05.002","volume":"5","author":"DD Thomas","year":"2015","unstructured":"Thomas DD (2015) Breathing new life into nitric oxide signaling: A brief overview of the interplay between oxygen and nitric oxide. Redox Biol 5: 225\u2013233","journal-title":"Redox Biol"},{"key":"BFEMBR201847047_CR49","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1038\/nature13852","volume":"515","author":"S Durdu","year":"2014","unstructured":"Durdu S, Iskar M, Revenu C, Schieber N, Kunze A, Bork P, Schwab Y, Gilmour D (2014) Luminal signalling links cell communication to tissue architecture during organogenesis. Nature 515: 120\u2013124","journal-title":"Nature"},{"key":"BFEMBR201847047_CR50","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.cbpa.2013.05.027","volume":"166","author":"F Syeda","year":"2013","unstructured":"Syeda F, Hauton D, Young S, Egginton S (2013) How ubiquitous is endothelial NOS? Comp Biochem Physiol A Mol Integr Physiol 166: 207\u2013214","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"BFEMBR201847047_CR51","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.freeradbiomed.2007.05.025","volume":"43","author":"S Lepiller","year":"2007","unstructured":"Lepiller S, Laurens V, Bouchot A, Herbomel P, Solary E, Chluba J (2007) Imaging of nitric oxide in a living vertebrate using a diamino\u2010fluorescein probe. Free Radic Biol Med 43: 619\u2013627","journal-title":"Free Radic Biol Med"},{"key":"BFEMBR201847047_CR52","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/383707a0","volume":"383","author":"A Joutel","year":"1996","unstructured":"Joutel A, Corpechot C, Ducros A, Vahedi K, Chabriat H, Mouton P, Alamowitch S, Domenga V, C\u00e9cillion M, Marechal E et\u00a0al (1996) Notch3 mutations in CADASIL, a hereditary adult\u2010onset condition causing stroke and dementia. Nature 383: 707\u2013710","journal-title":"Nature"},{"key":"BFEMBR201847047_CR53","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1172\/JCI8047","volume":"105","author":"A Joutel","year":"2000","unstructured":"Joutel A, Andreux F, Gaulis S, Domenga V, Cecillon M, Battail N, Piga N, Chapon F, Godfrain C, Tournier\u2010Lasserve E (2000) The ectodomain of the Notch3 receptor accumulates within the cerebrovasculature of CADASIL patients. J Clin Invest 105: 597\u2013605","journal-title":"J Clin Invest"},{"key":"BFEMBR201847047_CR54","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s00018-018-2934-5","volume":"76","author":"P Karschnia","year":"2019","unstructured":"Karschnia P, Nishimura S, Louvi A (2019) Cerebrovascular disorders associated with genetic lesions. Cell Mol Life Sci 76: 283\u2013300","journal-title":"Cell Mol Life Sci"},{"key":"BFEMBR201847047_CR55","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.devcel.2014.12.016","volume":"32","author":"M Renz","year":"2015","unstructured":"Renz M, Otten C, Faurobert E, Rudolph F, Zhu Y, Boulday G, Duchene J, Mickoleit M, Dietrich AC, Ramspacher C et\u00a0al (2015) Regulation of \u03b21 integrin\u2010Klf2\u2010mediated angiogenesis by CCM proteins. Dev Cell 32: 181\u2013190","journal-title":"Dev Cell"},{"key":"BFEMBR201847047_CR56","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/jcmm.12022","volume":"17","author":"C You","year":"2013","unstructured":"You C, Sandalcioglu IE, Dammann P, Felbor U, Sure U, Zhu Y (2013) Loss of CCM3 impairs DLL4\u2010Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations. J Cell Mol Med 17: 407\u2013418","journal-title":"J Cell Mol Med"},{"key":"BFEMBR201847047_CR57","volume-title":"The zebrafish book: a guide for laboratory use of Zebrafish (Brachydanio rerio)","author":"M Westerfield","year":"1993","unstructured":"Westerfield M (1993) The zebrafish book: a guide for laboratory use of Zebrafish (Brachydanio rerio). Eugene, OR: University of Oregon Press"},{"key":"BFEMBR201847047_CR58","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1101\/gad.1629408","volume":"22","author":"NC Chi","year":"2008","unstructured":"Chi NC, Shaw RM, De Val S, Kang G, Jan LY, Black BL, Stainier DY (2008) Foxn4 directly regulates tbx2b expression and atrioventricular canal formation. Genes Dev 22: 734\u2013739","journal-title":"Genes Dev"},{"key":"BFEMBR201847047_CR59","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/291293a0","volume":"291","author":"G Streisinger","year":"1981","unstructured":"Streisinger G, Walker C, Dower N, Knauber D, Singer F (1981) Production of homozygous diploid zebra fish (Brachydanio rerio). Nature 291: 293\u2013296","journal-title":"Nature"},{"key":"BFEMBR201847047_CR60","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.stem.2007.11.002","volume":"2","author":"RM White","year":"2008","unstructured":"White RM, Sessa A, Burke C, Bowman T, LeBlanc J, Ceol C, Bourque C, Dovey M, Goessling W, Burns CE et\u00a0al (2008) Transparent adult zebrafish as a tool for in\u00a0vivo transplantation analysis. Cell Stem Cell 2: 183\u2013189","journal-title":"Cell Stem Cell"},{"key":"BFEMBR201847047_CR61","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1038\/ni1007","volume":"4","author":"D Traver","year":"2003","unstructured":"Traver D, Paw BH, Poss KD, Penberthy WT, Lin S, Zon LI (2003) Transplantation and in\u00a0vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nat Immunol 4: 1238\u20131246","journal-title":"Nat Immunol"},{"key":"BFEMBR201847047_CR62","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.ydbio.2008.01.038","volume":"316","author":"Y Blum","year":"2008","unstructured":"Blum Y, Belting HG, Ellertsdottir E, Herwig L, L\u00fcders F, Affolter M (2008) Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo. Dev Biol 316: 312\u2013322","journal-title":"Dev Biol"},{"key":"BFEMBR201847047_CR63","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3390\/jdb6010006","volume":"6","author":"K Bergmann","year":"2018","unstructured":"Bergmann K, Santoscoy PM, Lygdas K, Nikolaeva Y, MacDonald RB, Cunliffe VT, Nikolaev A (2018) Imaging neuronal activity in the optic tectum of late stage larval zebrafish. J Dev Biol 6: 6","journal-title":"J Dev Biol"},{"key":"BFEMBR201847047_CR64","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1242\/dev.076000","volume":"139","author":"N Ninov","year":"2012","unstructured":"Ninov N, Borius M, Stainier DYR (2012) Different levels of Notch signaling regulate quiescence, renewal and differentiation in pancreatic endocrine progenitors. Development 139: 1557\u20131567","journal-title":"Development"},{"key":"BFEMBR201847047_CR65","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1242\/dev.105031","volume":"141","author":"A van Impel","year":"2014","unstructured":"van Impel A, Zhao Z, Hermkens DM, Roukens MG, Fischer JC, Peterson\u2010Maduro J, Duckers H, Ober EA, Ingham PW, Schulte\u2010Merker S (2014) Divergence of zebrafish and mouse lymphatic cell fate specification pathways. Development 141: 1228\u20131238","journal-title":"Development"},{"key":"BFEMBR201847047_CR66","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1160\/TH10-08-0525","volume":"105","author":"C Gray","year":"2011","unstructured":"Gray C, Loynes CA, Whyte MK, Crossman DC, Renshaw SA, Chico TJ (2011) Simultaneous intravital imaging of macrophage and neutrophil behaviour during inflammation using a novel transgenic zebrafish. Thromb Haemost 105: 811\u2013819","journal-title":"Thromb Haemost"},{"key":"BFEMBR201847047_CR67","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1364\/OL.32.002608","volume":"32","author":"J Huisken","year":"2007","unstructured":"Huisken J, Stainier DYR (2007) Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM). Opt Lett 32: 2608\u20132610","journal-title":"Opt Lett"},{"key":"BFEMBR201847047_CR68","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda\u2010Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B et\u00a0al (2012) Fiji \u2010 an open source platform for biological image analysis. Nat Methods 9: 676\u2013682","journal-title":"Nat Methods"},{"key":"BFEMBR201847047_CR69","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1093\/bioinformatics\/btp184","volume":"25","author":"S Preibisch","year":"2009","unstructured":"Preibisch S, Saalfeld S, Tomancak P (2009) Globally optimal stitching of tiled 3D microscopic image acquisitions. Bioinformatics 25: 1463\u20131465","journal-title":"Bioinformatics"},{"key":"BFEMBR201847047_CR70","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1242\/dev.082586","volume":"139","author":"A Kaufmann","year":"2012","unstructured":"Kaufmann A, Mickoleit M, Weber M, Huisken J (2012) Multilayer mounting enables long\u2010term imaging of zebrafish development in a light sheet microscope. Development 139: 3242\u20133247","journal-title":"Development"},{"key":"BFEMBR201847047_CR71","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/978-1-61779-523-7_6","volume":"843","author":"CE Schmitt","year":"2012","unstructured":"Schmitt CE, Holland MB, Jin SW (2012) Visualizing vascular networks in zebrafish: an introduction to microangiography. Methods Mol Biol 843: 59\u201367","journal-title":"Methods Mol Biol"},{"key":"BFEMBR201847047_CR72","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/nm1195-1143","volume":"1","author":"BM Weinstein","year":"1995","unstructured":"Weinstein BM, Stemple DL, Driever W, Fishman MC (1995) Gridlock, a localized heritable vascular patterning defect in the zebrafish. Nat Med 1: 1143\u20131147","journal-title":"Nat Med"},{"key":"BFEMBR201847047_CR73","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1242\/dev.100495","volume":"141","author":"L Le Guen","year":"2014","unstructured":"Le Guen L, Karpanen T, Schulte D, Harris NC, Koltowska K, Roukens G, Bower NI, van Impel A, Stacker SA, Achen MG et\u00a0al (2014) Ccbe1 regulates Vegfc\u2010mediated induction of Vegfr3 signaling during embryonic lymphangiogenesis. Development 141: 1239\u20131249","journal-title":"Development"},{"key":"BFEMBR201847047_CR74","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1161\/ATVBAHA.110.203034","volume":"30","author":"I Geudens","year":"2010","unstructured":"Geudens I, Herpers R, Hermans K, Segura I, Ruiz de Almodovar C, Bussmann J, De Smet F, Vandevelde W, Hogan BM, Siekmann A et\u00a0al (2010) Role of delta\u2010like\u20104\/Notch in the formation and wiring of the lymphatic network in zebrafish. Arterioscler Thromb Vasc Biol 30: 1695\u20131702","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"BFEMBR201847047_CR75","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1242\/dev.099986","volume":"141","author":"A Quillien","year":"2014","unstructured":"Quillien A, Moore JC, Shin M, Siekmann AF, Smith T, Pan L, Moens CB, Parsons MJ, Lawson ND (2014) Distinct Notch signaling outputs pattern the developing arterial system. Development 141: 1544\u20131552","journal-title":"Development"},{"key":"BFEMBR201847047_CR76","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/35014075","volume":"2","author":"WM Morton","year":"2000","unstructured":"Morton WM, Ayscough KR, McLaughlin PJ (2000) Latrunculin alters the actin\u2010monomer subunit interface to prevent polymerization. Nat Cell Biol 2: 376\u2013378","journal-title":"Nat Cell Biol"},{"key":"BFEMBR201847047_CR77","doi-asserted-by":"crossref","first-page":"35557","DOI":"10.1074\/jbc.M405319200","volume":"279","author":"M Kov\u00e1cs","year":"2004","unstructured":"Kov\u00e1cs M, T\u00f3th J, Het\u00e9nyi C, M\u00e1ln\u00e1si\u2010Csizmadia A, Sellers JR (2004) Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279: 35557\u201335563","journal-title":"J Biol Chem"},{"key":"BFEMBR201847047_CR78","doi-asserted-by":"crossref","first-page":"9134","DOI":"10.1158\/0008-5472.CAN-05-4290","volume":"66","author":"K Nakamura","year":"2006","unstructured":"Nakamura K, Taguchi E, Miura T, Yamamoto A, Takahashi K, Bichat F, Guilbaud N, Hasegawa K, Kubo K, Fujiwara Y et\u00a0al (2006) KRN951, a highly potent inhibitor of vascular endothelial growth factor receptor tyrosine kinases, has antitumor activities and affects functional vascular properties. Cancer Res 66: 9134\u20139142","journal-title":"Cancer Res"},{"key":"BFEMBR201847047_CR79","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1093\/embo-reports\/kvf124","volume":"3","author":"A Geling","year":"2002","unstructured":"Geling A, Steiner H, Willem M, Bally\u2010Cuif L, Haass C (2002) A gamma\u2010secretase inhibitor blocks Notch signaling in\u00a0vivo and causes a severe neurogenic phenotype in zebrafish. EMBO Rep 3: 688\u2013694","journal-title":"EMBO Rep"}],"container-title":["The EMBO Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embr.201847047.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.15252\/embr.201847047","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/embr.201847047\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/embr.201847047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embr.201847047.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T13:31:53Z","timestamp":1779111113000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/embr.201847047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,18]]},"references-count":79,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8,1]]}},"alternative-id":["BFEMBR201847047"],"URL":"https:\/\/doi.org\/10.15252\/embr.201847047","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/416206","asserted-by":"object"}]},"ISSN":["1469-221X","1469-3178"],"issn-type":[{"value":"1469-221X","type":"print"},{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,18]]},"assertion":[{"value":"12 September 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 May 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 May 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 June 2019","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}],"article-number":"EMBR201847047"}}