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Here, we report a simple optochemical method to induce cell contractions\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    during\n                    <jats:italic>Drosophila<\/jats:italic>\n                    morphogenesis at single\u2010cell resolution. We employed the photolabile Ca\n                    <jats:sup>2+<\/jats:sup>\n                    chelator\n                    <jats:italic>o<\/jats:italic>\n                    \u2010nitrophenyl EGTA to induce bursts of intracellular free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    by laser photolysis in the epithelial tissue. Ca\n                    <jats:sup>2+<\/jats:sup>\n                    bursts appear within seconds and are restricted to individual target cells. Cell contraction reliably followed within a minute, causing an approximately 50% drop in the cross\u2010sectional area. Increased Ca\n                    <jats:sup>2+<\/jats:sup>\n                    levels are reversible, and the target cells further participated in tissue morphogenesis. Depending on Rho kinase (ROCK) activity but not RhoGEF2, cell contractions are paralleled with non\u2010muscle myosin II accumulation in the apico\u2010medial cortex, indicating that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    bursts trigger non\u2010muscle myosin II activation. Our approach can be, in principle, adapted to many experimental systems and species, as no specific genetic elements are required.\n                  <\/jats:p>","DOI":"10.15252\/embr.201947755","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T14:38:16Z","timestamp":1575643096000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["In vivo optochemical control of cell contractility at single\u2010cell resolution"],"prefix":"10.1038","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0672-4002","authenticated-orcid":false,"given":"Deqing","family":"Kong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"H\u00e4ring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1114-9233","authenticated-orcid":false,"given":"J\u00f6rg","family":"Gro\u00dfhans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,30]]},"reference":[{"key":"BFEMBR201947755_CR1","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/nature02590","volume":"429","author":"C Bertet","year":"2004","unstructured":"Bertet C, Sulak L, Lecuit T (2004) Myosin\u2010dependent junction remodelling controls planar cell intercalation and axis elongation. 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