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This circular junction stretches while the parasites apply a traction force to pass through, a step that typically concurs with transient constriction of the parasite body. Here we analyse F\u2010actin dynamics during host cell invasion. Super\u2010resolution microscopy and real\u2010time imaging highlighted an F\u2010actin pool at the apex of pre\u2010invading parasite, an F\u2010actin ring at the junction area during invasion but also networks of perinuclear and posteriorly localised F\u2010actin. Mutant parasites with dysfunctional acto\u2010myosin showed significant decrease of junctional and perinuclear F\u2010actin and are coincidently affected in nuclear passage through the junction. We propose that the F\u2010actin machinery eases nuclear passage by stabilising the junction and pushing the nucleus through the constriction. Our analysis suggests that the junction opposes resistance to the passage of the parasite's nucleus and provides the first evidence for a dual contribution of actin\u2010forces during host cell invasion by apicomplexan parasites.\n                  <\/jats:p>","DOI":"10.15252\/embr.201948896","type":"journal-article","created":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T10:38:36Z","timestamp":1574937516000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Apicomplexan F\u2010actin is required for efficient nuclear entry during host cell invasion"],"prefix":"10.1038","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0430-1463","authenticated-orcid":false,"given":"Mario","family":"Del Rosario","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Periz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Pavlou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Lyth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9280-705X","authenticated-orcid":false,"given":"Fernanda","family":"Latorre\u2010Barragan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujaan","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gurman S","family":"Pall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5928-1850","authenticated-orcid":false,"given":"Johannes Felix","family":"Stortz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro","family":"Lemgruber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie A","family":"Whitelaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0275-352X","authenticated-orcid":false,"given":"Jake","family":"Baum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5677-7463","authenticated-orcid":false,"given":"Isabelle","family":"Tardieux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4816-5221","authenticated-orcid":false,"given":"Markus","family":"Meissner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,4]]},"reference":[{"key":"BFEMBR201948896_CR1","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.mib.2013.05.002","volume":"16","author":"M Meissner","year":"2013","unstructured":"Meissner M, Ferguson DJP, Frischknecht F (2013) Invasion factors of apicomplexan parasites: essential or redundant? 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