{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:32:41Z","timestamp":1791959561666,"version":"4.3.4"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T00:00:00Z","timestamp":1652659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T00:00:00Z","timestamp":1652659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G.0C49.13N"],"award-info":[{"award-number":["G.0C49.13N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["FWOOPR2018010801"],"award-info":[{"award-number":["FWOOPR2018010801"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["3G.0448.18"],"award-info":[{"award-number":["3G.0448.18"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["3G.0447.18"],"award-info":[{"award-number":["3G.0447.18"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007229","name":"UGent|Bijzonder Onderzoeksfonds UGent","doi-asserted-by":"publisher","award":["BOF24Y2019003201"],"award-info":[{"award-number":["BOF24Y2019003201"]}],"id":[{"id":"10.13039\/501100007229","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Rep"],"published-print":{"date-parts":[[2022,7,5]]},"DOI":"10.15252\/embr.202154339","type":"journal-article","created":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T05:26:06Z","timestamp":1652678766000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Nlrp3 inflammasome activation in macrophages suffices for inducing autoinflammation in mice"],"prefix":"10.1038","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2314-7326","authenticated-orcid":false,"given":"Ulrika C","family":"Frising","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-827X","authenticated-orcid":false,"given":"Silvia","family":"Ribo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5499-0252","authenticated-orcid":false,"given":"M Giulia","family":"Doglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-9342","authenticated-orcid":false,"given":"Bernard","family":"Malissen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8427-4775","authenticated-orcid":false,"given":"Geert","family":"van Loo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5012-654X","authenticated-orcid":false,"given":"Andy","family":"Wullaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"key":"BFEMBR202154339_CR1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jim.2014.05.009","volume":"408","author":"CL Abram","year":"2014","unstructured":"Abram CL, Roberge GL, Hu Y, Lowell CA (2014) Comparative analysis of the efficiency and specificity of myeloid\u2010Cre deleting strains using ROSA\u2010EYFP reporter mice. J Immunol Methods 408: 89\u2013100","journal-title":"J Immunol Methods"},{"key":"BFEMBR202154339_CR2","doi-asserted-by":"crossref","first-page":"3340","DOI":"10.1002\/art.10688","volume":"46","author":"I Aksentijevich","year":"2002","unstructured":"Aksentijevich I, Nowak M, Mallah M, Chae JJ, Watford WT, Hofmann SR, Stein L, Russo R, Goldsmith D, Dent P et al (2002) De novo CIAS1 mutations, cytokine activation, and evidence for genetic heterogeneity in patients with neonatal\u2010onset multisystem inflammatory disease (NOMID): a new member of the expanding family of pyrin\u2010associated autoinflammatory diseases. Arthritis Rheum 46: 3340\u20133348","journal-title":"Arthritis Rheum"},{"key":"BFEMBR202154339_CR3","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s10875-019-00638-z","volume":"39","author":"LM Booshehri","year":"2019","unstructured":"Booshehri LM, Hoffman HM (2019) CAPS and NLRP3. J Clin Immunol 39: 277\u2013286","journal-title":"J Clin Immunol"},{"key":"BFEMBR202154339_CR4","doi-asserted-by":"crossref","first-page":"4695","DOI":"10.1172\/JCI71543","volume":"123","author":"SD Brydges","year":"2013","unstructured":"Brydges SD, Broderick L, McGeough MD, Pena CA, Mueller JL, Hoffman HM (2013) Divergence of IL\u20101, IL\u201018, and cell death in NLRP3 inflammasomopathies. J Clin Invest 123: 4695\u20134705","journal-title":"J Clin Invest"},{"key":"BFEMBR202154339_CR5","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.immuni.2009.05.005","volume":"30","author":"SD Brydges","year":"2009","unstructured":"Brydges SD, Mueller JL, McGeough MD, Pena CA, Misaghi A, Gandhi C, Putnam CD, Boyle DL, Firestein GS, Horner AA et al (2009) Inflammasome\u2010mediated disease animal models reveal roles for innate but not adaptive immunity. Immunity 30: 875\u2013887","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR6","doi-asserted-by":"publisher","DOI":"10.1002\/hep.32320","author":"DM Calcagno","year":"2022","unstructured":"Calcagno DM, Chu A, Gaul S, Taghdiri N, Toomu A, Leszczynska A, Kaufmann B, Papouchado B, Wree A, Geisler L et al (2022) NOD\u2010like receptor protein 3 activation causes spontaneous inflammation and fibrosis that mimics human NASH. Hepatology, https:\/\/doi.org\/10.1002\/hep.32320","journal-title":"Hepatology"},{"key":"BFEMBR202154339_CR7","doi-asserted-by":"crossref","first-page":"4561","DOI":"10.1172\/JCI134778","volume":"130","author":"G Camilli","year":"2020","unstructured":"Camilli G, Bohm M, Piffer AC, Lavenir R, Williams DL, Neven B, Grateau G, Georgin\u2010Lavialle S, Quintin J (2020) beta\u2010Glucan\u2010induced reprogramming of human macrophages inhibits NLRP3 inflammasome activation in cryopyrinopathies. J Clin Invest 130: 4561\u20134573","journal-title":"J Clin Invest"},{"key":"BFEMBR202154339_CR8","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/s41590-019-0313-5","volume":"20","author":"Z Erlich","year":"2019","unstructured":"Erlich Z, Shlomovitz I, Edry\u2010Botzer L, Cohen H, Frank D, Wang H, Lew AM, Lawlor KE, Zhan Y, Vince JE et al (2019) Macrophages, rather than DCs, are responsible for inflammasome activity in the GM\u2010CSF BMDC model. Nat Immunol 20: 397\u2013406","journal-title":"Nat Immunol"},{"key":"BFEMBR202154339_CR9","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.immuni.2018.02.002","volume":"48","author":"M Evrard","year":"2018","unstructured":"Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S et al (2018) Developmental analysis of bone marrow neutrophils reveals populations specialized in expansion, trafficking, and effector functions. Immunity 48: 364\u2013379.e8","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR10","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1086\/341357","volume":"71","author":"J Feldmann","year":"2002","unstructured":"Feldmann J, Prieur AM, Quartier P, Berquin P, Certain S, Cortis E, Teillac\u2010Hamel D, Fischer A, de Saint BG (2002) Chronic infantile neurological cutaneous and articular syndrome is caused by mutations in CIAS1, a gene highly expressed in polymorphonuclear cells and chondrocytes. Am J Hum Genet 71: 198\u2013203","journal-title":"Am J Hum Genet"},{"key":"BFEMBR202154339_CR11","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1038\/ni.2419","volume":"13","author":"EL Gautier","year":"2012","unstructured":"Gautier EL, Shay T, Miller J, Greter M, Jakubzick C, Ivanov S, Helft J, Chow A, Elpek KG, Gordonov S et al (2012) Gene\u2010expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nat Immunol 13: 1118\u20131128","journal-title":"Nat Immunol"},{"key":"BFEMBR202154339_CR12","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.immuni.2018.10.005","volume":"49","author":"M Guilliams","year":"2018","unstructured":"Guilliams M, Mildner A, Yona S (2018) Developmental and functional heterogeneity of monocytes. Immunity 49: 595\u2013613","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR13","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.immuni.2020.02.015","volume":"52","author":"M Guilliams","year":"2020","unstructured":"Guilliams M, Thierry GR, Bonnardel J, Bajenoff M (2020) Establishment and maintenance of the macrophage niche. Immunity 52: 434\u2013451","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jaci.2016.05.001","volume":"138","author":"HM Hoffman","year":"2016","unstructured":"Hoffman HM, Broderick L (2016) The role of the inflammasome in patients with autoinflammatory diseases. J Allergy Clin Immunol 138: 3\u201314","journal-title":"J Allergy Clin Immunol"},{"key":"BFEMBR202154339_CR15","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/ng756","volume":"29","author":"HM Hoffman","year":"2001","unstructured":"Hoffman HM, Mueller JL, Broide DH, Wanderer AA, Kolodner RD (2001a) Mutation of a new gene encoding a putative pyrin\u2010like protein causes familial cold autoinflammatory syndrome and Muckle\u2010Wells syndrome. Nat Genet 29: 301\u2013305","journal-title":"Nat Genet"},{"key":"BFEMBR202154339_CR16","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1067\/mai.2001.118790","volume":"108","author":"HM Hoffman","year":"2001","unstructured":"Hoffman HM, Wanderer AA, Broide DH (2001b) Familial cold autoinflammatory syndrome: phenotype and genotype of an autosomal dominant periodic fever. J Allergy Clin Immunol 108: 615\u2013620","journal-title":"J Allergy Clin Immunol"},{"key":"BFEMBR202154339_CR17","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1038\/s41590-021-00968-4","volume":"22","author":"TE Khoyratty","year":"2021","unstructured":"Khoyratty TE, Ai Z, Ballesteros I, Eames HL, Mathie S, Mart\u00edn\u2010Salamanca S, Wang L, Hemmings A, Willemsen N, von Werz V et al (2021) Distinct transcription factor networks control neutrophil\u2010driven inflammation. Nat Immunol 22: 1093\u20131106","journal-title":"Nat Immunol"},{"key":"BFEMBR202154339_CR18","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.immuni.2020.06.005","volume":"53","author":"I Kwok","year":"2020","unstructured":"Kwok I, Becht E, Xia YU, Ng M, Teh YC, Tan L, Evrard M, Li JLY, Tran HTN, Tan Y et al (2020) Combinatorial single\u2010cell analyses of granulocyte\u2010monocyte progenitor heterogeneity reveals an early uni\u2010potent neutrophil progenitor. Immunity 53: 303\u2013318.e5","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR19","doi-asserted-by":"crossref","first-page":"2416","DOI":"10.1056\/NEJMoa0810787","volume":"360","author":"HJ Lachmann","year":"2009","unstructured":"Lachmann HJ, Kone\u2010Paut I, Kuemmerle\u2010Deschner JB, Leslie KS, Hachulla E, Quartier P, Gitton X, Widmer A, Patel N, Hawkins PN et al (2009) Use of canakinumab in the cryopyrin\u2010associated periodic syndrome. N Engl J Med 360: 2416\u20132425","journal-title":"N Engl J Med"},{"key":"BFEMBR202154339_CR20","doi-asserted-by":"crossref","first-page":"2707","DOI":"10.4049\/jimmunol.1101737","volume":"189","author":"MD McGeough","year":"2012","unstructured":"McGeough MD, Pena CA, Mueller JL, Pociask DA, Broderick L, Hoffman HM, Brydges SD (2012) Cutting edge: IL\u20106 is a marker of inflammation with no direct role in inflammasome\u2010mediated mouse models. J Immunol 189: 2707\u20132711","journal-title":"J Immunol"},{"key":"BFEMBR202154339_CR21","doi-asserted-by":"crossref","first-page":"4488","DOI":"10.1172\/JCI90699","volume":"127","author":"MD McGeough","year":"2017","unstructured":"McGeough MD, Wree A, Inzaugarat ME, Haimovich A, Johnson CD, Pena CA, Goldbach\u2010Mansky R, Broderick L, Feldstein AE, Hoffman HM (2017) TNF regulates transcription of NLRP3 inflammasome components and inflammatory molecules in cryopyrinopathies. J Clin Invest 127: 4488\u20134497","journal-title":"J Clin Invest"},{"key":"BFEMBR202154339_CR22","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.immuni.2012.04.013","volume":"37","author":"Y Nakamura","year":"2012","unstructured":"Nakamura Y, Franchi L, Kambe N, Meng G, Strober W, Nunez G (2012) Critical role for mast cells in interleukin\u20101beta\u2010driven skin inflammation associated with an activating mutation in the nlrp3 protein. Immunity 37: 85\u201395","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR23","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.1038\/s41467-019-11053-8","volume":"10","author":"R Rojo","year":"2019","unstructured":"Rojo R, Raper A, Ozdemir DD, Lefevre L, Grabert K, Wollscheid\u2010Lengeling E, Bradford B, Caruso M, Gazova I, S\u00e1nchez A et al (2019) Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations. Nat Commun 10: 3215","journal-title":"Nat Commun"},{"key":"BFEMBR202154339_CR24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0022-1759(94)90012-4","volume":"174","author":"N Van Rooijen","year":"1994","unstructured":"Van Rooijen N, Sanders A (1994) Liposome mediated depletion of macrophages: mechanism of action, preparation of liposomes and applications. J Immunol Methods 174: 83\u201393","journal-title":"J Immunol Methods"},{"key":"BFEMBR202154339_CR25","doi-asserted-by":"crossref","first-page":"e123294","DOI":"10.1172\/jci.insight.123294","volume":"5","author":"S Schuster\u2010Gaul","year":"2020","unstructured":"Schuster\u2010Gaul S, Geisler LJ, McGeough MD, Johnson CD, Zagorska A, Li LI, Wree A, Barry V, Mikaelian I, Jih LJ et al (2020) ASK1 inhibition reduces cell death and hepatic fibrosis in an Nlrp3 mutant liver injury model. JCI Insight 5: e123294","journal-title":"JCI Insight"},{"key":"BFEMBR202154339_CR26","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.immuni.2018.07.004","volume":"49","author":"CL Scott","year":"2018","unstructured":"Scott CL, T\u2019Jonck W, Martens L, Todorov H, Sichien D, Soen B, Bonnardel J, De Prijck S, Vandamme N, Cannoodt R et al (2018) The transcription factor ZEB2 is required to maintain the tissue\u2010specific identities of macrophages. Immunity 49: 312\u2013325.e5","journal-title":"Immunity"},{"key":"BFEMBR202154339_CR27","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20201466","volume":"218","author":"J Stackowicz","year":"2021","unstructured":"Stackowicz J, Gaudenzio N, Serhan N, Conde E, Godon O, Marichal T, Starkl P, Balbino B, Roers A, Bruhns P et al (2021) Neutrophil\u2010specific gain\u2010of\u2010function mutations in Nlrp3 promote development of cryopyrin\u2010associated periodic syndrome. J Exp Med 218: e20201466","journal-title":"J Exp Med"},{"key":"BFEMBR202154339_CR28","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1002\/humu.20080","volume":"24","author":"I Touitou","year":"2004","unstructured":"Touitou I, Lesage S, McDermott M, Cuisset L, Hoffman H, Dode C, Shoham N, Aganna E, Hugot J\u2010P, Wise C et al (2004) Infevers: an evolving mutation database for auto\u2010inflammatory syndromes. Hum Mutat 24: 194\u2013198","journal-title":"Hum Mutat"},{"key":"BFEMBR202154339_CR29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000354","volume":"17","author":"L Vande Walle","year":"2019","unstructured":"Vande Walle L, Stowe IB, \u0160\u00e1cha P, Lee BL, Demon D, Fossoul A, Van Hauwermeiren F, Saavedra PHV, \u0160imon P, \u0160ubrt V et al (2019) MCC950\/CRID3 potently targets the NACHT domain of wild\u2010type NLRP3 but not disease\u2010associated mutants for inflammasome inhibition. PLoS Biol 17: e3000354","journal-title":"PLoS Biol"},{"key":"BFEMBR202154339_CR30","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/nature05541","volume":"445","author":"A Ventura","year":"2007","unstructured":"Ventura A, Kirsch DG, McLaughlin ME, Tuveson DA, Grimm J, Lintault L, Newman J, Reczek EE, Weissleder R, Jacks T (2007) Restoration of p53 function leads to tumour regression in vivo. Nature 445: 661\u2013665","journal-title":"Nature"},{"key":"BFEMBR202154339_CR31","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1681\/ASN.2010020143","volume":"21","author":"A Vilaysane","year":"2010","unstructured":"Vilaysane A, Chun J, Seamone ME, Wang W, Chin R, Hirota S, Li Y, Clark SA, Tschopp J, Trpkov K et al (2010) The NLRP3 inflammasome promotes renal inflammation and contributes to CKD. J Am Soc Nephrol 21: 1732\u20131744","journal-title":"J Am Soc Nephrol"},{"key":"BFEMBR202154339_CR32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000047","volume":"16","author":"J Xiao","year":"2018","unstructured":"Xiao J, Wang C, Yao J\u2010C, Alippe Y, Xu C, Kress D, Civitelli R, Abu\u2010Amer Y, Kanneganti T\u2010D, Link DC et al (2018) Gasdermin D mediates the pathogenesis of neonatal\u2010onset multisystem inflammatory disease in mice. PLoS Biol 16: e3000047","journal-title":"PLoS Biol"},{"key":"BFEMBR202154339_CR33","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.immuni.2017.10.021","volume":"47","author":"A Yanez","year":"2017","unstructured":"Yanez A, Coetzee SG, Olsson A, Muench DE, Berman BP, Hazelett DJ, Salomonis N, Grimes HL, Goodridge HS (2017) Granulocyte\u2010monocyte progenitors and monocyte\u2010dendritic cell progenitors independently produce functionally distinct monocytes. Immunity 47: 890\u2013902 e894","journal-title":"Immunity"}],"container-title":["The EMBO Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embr.202154339.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.15252\/embr.202154339","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/embr.202154339\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/embr.202154339","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embr.202154339.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:01:35Z","timestamp":1779112895000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/embr.202154339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,16]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7,5]]}},"alternative-id":["BFEMBR202154339"],"URL":"https:\/\/doi.org\/10.15252\/embr.202154339","relation":{},"ISSN":["1469-221X","1469-3178"],"issn-type":[{"value":"1469-221X","type":"print"},{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,16]]},"assertion":[{"value":"15 November 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 April 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 May 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosure and competing interests statement"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}],"article-number":"EMBR202154339"}}