{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:17:13Z","timestamp":1793060233548,"version":"4.5.0"},"reference-count":66,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["TTU 07.708"],"award-info":[{"award-number":["TTU 07.708"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI166638"],"award-info":[{"award-number":["AI166638"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI151436"],"award-info":[{"award-number":["AI151436"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["GM056927"],"award-info":[{"award-number":["GM056927"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI073898"],"award-info":[{"award-number":["AI073898"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI152543"],"award-info":[{"award-number":["AI152543"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI100853"],"award-info":[{"award-number":["AI100853"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI007180"],"award-info":[{"award-number":["AI007180"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Rep"],"published-print":{"date-parts":[[2023,12,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Unlike most RNA and DNA viruses that broadly stimulate mRNA decay and interfere with host gene expression, human cytomegalovirus (HCMV) extensively remodels the host translatome without producing an mRNA decay enzyme. By performing a targeted loss\u2010of\u2010function screen in primary human fibroblasts, we here identify the host CCR4\u2010NOT deadenylase complex members CNOT1 and CNOT3 as unexpected pro\u2010viral host factors that selectively regulate HCMV reproduction. We find that the scaffold subunit CNOT1 is specifically required for late viral gene expression and genome\u2010wide host responses in CCR4\u2010NOT\u2010disrupted cells. By profiling poly(A)\u2010tail lengths of individual HCMV and host mRNAs using nanopore direct RNA sequencing, we reveal poly(A)\u2010tails of viral messages to be markedly longer than those of cellular mRNAs and significantly less sensitive to CCR4\u2010NOT disruption. Our data establish that mRNA deadenylation by host CCR4\u2010NOT is critical for productive HCMV replication and define a new mechanism whereby herpesvirus infection subverts cellular mRNA metabolism to remodel the gene expression landscape of the infected cell. Moreover, we expose an unanticipated host factor with potential to become a therapeutic anti\u2010HCMV target.<\/jats:p>","DOI":"10.15252\/embr.202256327","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T04:04:47Z","timestamp":1697515487000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["CCR4\u2010NOT differentially controls host versus virus poly(a)\u2010tail length and regulates HCMV infection"],"prefix":"10.1038","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3577-4746","authenticated-orcid":false,"given":"Hannah M","family":"Burgess","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6840-6718","authenticated-orcid":false,"given":"Rebecca","family":"Grande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0575-0681","authenticated-orcid":false,"given":"Sofia","family":"Riccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2045-8816","authenticated-orcid":false,"given":"Ikshitaa","family":"Dinesh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0476-162X","authenticated-orcid":false,"given":"Gerlof Sebastiaan","family":"Winkler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4292-0599","authenticated-orcid":false,"given":"Daniel P","family":"Depledge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Mohr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,17]]},"reference":[{"key":"BFEMBR202256327_CR1","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1083\/jcb.96.3.717","volume":"96","author":"BW Baer","year":"1983","unstructured":"Baer BW, Kornberg RD (1983) The protein responsible for the repeating structure of cytoplasmic poly(A)\u2010ribonucleoprotein. J Cell Biol 96: 717\u2013721","journal-title":"J Cell Biol"},{"key":"BFEMBR202256327_CR2","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nsmb.3310","volume":"23","author":"R Batra","year":"2016","unstructured":"Batra R, Stark TJ, Clark E, Belzile JP, Wheeler EC, Yee BA, Huang H, Gelboin\u2010Burkhart C, Huelga SC, Aigner S et\u00a0al (2016) RNA\u2010binding protein CPEB1 remodels host and viral RNA landscapes. Nat Struct Mol Biol 23: 1101\u20131110","journal-title":"Nat Struct Mol Biol"},{"key":"BFEMBR202256327_CR3","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.49551","volume":"8","author":"C Bianco","year":"2019","unstructured":"Bianco C, Mohr I (2019) Ribosome biogenesis restricts innate immune responses to virus infection and DNA. Elife 8: e49551","journal-title":"Elife"},{"key":"BFEMBR202256327_CR4","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1172\/JCI45449","volume":"121","author":"M Boeckh","year":"2011","unstructured":"Boeckh M, Geballe AP (2011) Cytomegalovirus: pathogen, paradigm, and puzzle. J Clin Invest 121: 1673\u20131680","journal-title":"J Clin Invest"},{"key":"BFEMBR202256327_CR5","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1038\/nsmb.2681","volume":"20","author":"A Boland","year":"2013","unstructured":"Boland A, Chen Y, Raisch T, Jonas S, Kuzuo\u011flu\u2010\u00d6zt\u00fcrk D, Wohlbold L, Weichenrieder O, Izaurralde E (2013) Structure and assembly of the NOT module of the human CCR4\u2010NOT complex. Nat Struct Mol Biol 20: 1289\u20131297","journal-title":"Nat Struct Mol Biol"},{"key":"BFEMBR202256327_CR6","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.chom.2015.02.003","volume":"17","author":"HM Burgess","year":"2015","unstructured":"Burgess HM, Mohr I (2015) Cellular 5\u2032\u20103' mRNA exonuclease Xrn1 controls double\u2010stranded RNA accumulation and anti\u2010viral responses. Cell Host Microbe 17: 332\u2013344","journal-title":"Cell Host Microbe"},{"key":"BFEMBR202256327_CR7","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00829-18","volume":"92","author":"HM Burgess","year":"2018","unstructured":"Burgess HM, Mohr I (2018) Defining the role of stress granules in innate immune suppression by the herpes simplex virus 1 endoribonuclease VHS. J Virol 92: e00829\u201018","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR8","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1101\/gad.349276.121","volume":"36","author":"HM Burgess","year":"2022","unstructured":"Burgess HM, Vink EI, Mohr I (2022) Minding the message: tactics controlling RNA decay, modification, and translation in virus\u2010infected cells. Genes Dev 36: 108\u2013132","journal-title":"Genes Dev"},{"key":"BFEMBR202256327_CR9","doi-asserted-by":"crossref","DOI":"10.1126\/science.aay6912","volume":"368","author":"R Buschauer","year":"2020","unstructured":"Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R et\u00a0al (2020) The Ccr4\u2010Not complex monitors the translating ribosome for codon optimality. Science 368: eaay6912","journal-title":"Science"},{"key":"BFEMBR202256327_CR10","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02034-17","volume":"92","author":"N Chalabi Hagkarim","year":"2018","unstructured":"Chalabi Hagkarim N, Ryan EL, Byrd PJ, Hollingworth R, Shimwell NJ, Agathanggelou A, Vavasseur M, Kolbe V, Speiseder T, Dobner T et\u00a0al (2018) Degradation of a novel DNA damage response protein, tankyrase 1 binding protein 1, following adenovirus infection. J Virol 92: e02034\u201017","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR11","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1016\/j.molcel.2014.02.007","volume":"53","author":"H Chang","year":"2014","unstructured":"Chang H, Lim J, Ha M, Kim VN (2014) TAIL\u2010seq: genome\u2010wide determination of poly(A) tail length and 3\u2032 end modifications. Mol Cell 53: 1044\u20131052","journal-title":"Mol Cell"},{"key":"BFEMBR202256327_CR12","doi-asserted-by":"crossref","first-page":"5363","DOI":"10.1128\/JVI.00115-11","volume":"85","author":"B Dauber","year":"2011","unstructured":"Dauber B, Pelletier J, Smiley JR (2011) The herpes simplex virus 1 vhs protein enhances translation of viral true late mRNAs and virus production in a cell type\u2010dependent manner. J Virol 85: 5363\u20135373","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008111","volume":"15","author":"B Dauber","year":"2019","unstructured":"Dauber B, Saffran HA, Smiley JR (2019) The herpes simplex virus host shutoff (vhs) RNase limits accumulation of double stranded RNA in infected cells: evidence for accelerated decay of duplex RNA. PLoS Pathog 15: e1008111","journal-title":"PLoS Pathog"},{"key":"BFEMBR202256327_CR14","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1261\/rna.575107","volume":"13","author":"EL van Dijk","year":"2007","unstructured":"van Dijk EL, Schilders G, Pruijn GJM (2007) Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways. RNA 13: 1027\u20131035","journal-title":"RNA"},{"key":"BFEMBR202256327_CR15","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/wrna.56","volume":"2","author":"CR Eckmann","year":"2011","unstructured":"Eckmann CR, Rammelt C, Wahle E (2011) Control of poly(A) tail length. Wiley Interdiscip Rev RNA 2: 348\u2013361","journal-title":"Wiley Interdiscip Rev RNA"},{"key":"BFEMBR202256327_CR16","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1097\/00007890-199609150-00020","volume":"62","author":"LJ Geist","year":"1996","unstructured":"Geist LJ, Dai LY (1996) Cytomegalovirus modulates interleukin\u20106 gene expression. Transplantation 62: 653\u2013658","journal-title":"Transplantation"},{"key":"BFEMBR202256327_CR17","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.virol.2018.07.008","volume":"522","author":"S Gopal","year":"2018","unstructured":"Gopal S, Perez E Jr, Xia AY, Knowlton JJ, Cerqueira F, Dermody TS, Upton JW (2018) Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC\/CCT complex. Virology 522: 92\u2013105","journal-title":"Virology"},{"key":"BFEMBR202256327_CR18","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1002\/path.4437","volume":"235","author":"P Griffiths","year":"2015","unstructured":"Griffiths P, Baraniak I, Reeves M (2015) The pathogenesis of human cytomegalovirus. J Pathol 235: 288\u2013297","journal-title":"J Pathol"},{"key":"BFEMBR202256327_CR19","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005748","volume":"12","author":"T Harwardt","year":"2016","unstructured":"Harwardt T, Lukas S, Zenger M, Reitberger T, Danzer D, \u00dcbner T, Munday DC, Nevels M, Paulus C (2016) Human cytomegalovirus immediate\u2010early 1 protein rewires upstream STAT3 to downstream STAT1 signaling switching an IL6\u2010type to an IFN\u03b3\u2010like response. PLoS Pathog 12: e1005748","journal-title":"PLoS Pathog"},{"key":"BFEMBR202256327_CR20","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1038\/s41587-021-01059-3","volume":"40","author":"MY Hein","year":"2022","unstructured":"Hein MY, Weissman JS (2022) Functional single\u2010cell genomics of human cytomegalovirus infection. Nat Biotechnol 40: 391\u2013401","journal-title":"Nat Biotechnol"},{"key":"BFEMBR202256327_CR21","doi-asserted-by":"crossref","DOI":"10.3390\/v13091836","volume":"13","author":"T Hennig","year":"2021","unstructured":"Hennig T, Djakovic L, D\u00f6lken L, Whisnant AW (2021) A review of the multipronged attack of herpes simplex virus 1 on the host transcriptional machinery. Viruses 13: 1836","journal-title":"Viruses"},{"key":"BFEMBR202256327_CR22","doi-asserted-by":"crossref","first-page":"10490","DOI":"10.1038\/s41598-022-14362-z","volume":"12","author":"KL Hsu","year":"2022","unstructured":"Hsu KL, Yen HS, Yeang CH (2022) Cooperative stability renders protein complex formation more robust and controllable. Sci Rep 12: 10490","journal-title":"Sci Rep"},{"key":"BFEMBR202256327_CR23","doi-asserted-by":"crossref","first-page":"4219","DOI":"10.1016\/j.bmcl.2015.07.095","volume":"25","author":"GP Jadhav","year":"2015","unstructured":"Jadhav GP, Kaur I, Maryati M, Airhihen B, Fischer PM, Winkler GS (2015) Discovery, synthesis and biochemical profiling of purine\u20102,6\u2010dione derivatives as inhibitors of the human poly(A)\u2010selective ribonuclease Caf1. Bioorg Med Chem Lett 25: 4219\u20134224","journal-title":"Bioorg Med Chem Lett"},{"key":"BFEMBR202256327_CR24","first-page":"D498","volume":"48","author":"B Jassal","year":"2020","unstructured":"Jassal B, Matthews L, Viteri G, Gong C, Lorente P, Fabregat A, Sidiropoulos K, Cook J, Gillespie M, Haw R et\u00a0al (2020) The reactome pathway knowledgebase. Nucleic Acids Res 48: D498\u2013D503","journal-title":"Nucleic Acids Res"},{"key":"BFEMBR202256327_CR25","doi-asserted-by":"crossref","DOI":"10.12703\/r\/10-71","volume":"10","author":"AD Keegan","year":"2021","unstructured":"Keegan AD, Leonard WJ, Zhu J (2021) Recent advances in understanding the role of IL\u20104 signaling. Fac Rev 10: 71","journal-title":"Fac Rev"},{"key":"BFEMBR202256327_CR26","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/s41594-020-0427-3","volume":"27","author":"D Kim","year":"2020","unstructured":"Kim D, Lee YS, Jung SJ, Yeo J, Seo JJ, Lee YY, Lim J, Chang H, Song J, Yang J et\u00a0al (2020) Viral hijacking of the TENT4\u2010ZCCHC14 complex protects viral RNAs via mixed tailing. Nat Struct Mol Biol 27: 581\u2013588","journal-title":"Nat Struct Mol Biol"},{"key":"BFEMBR202256327_CR27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.bbaexp.2004.03.008","volume":"1678","author":"U K\u00fchn","year":"2004","unstructured":"K\u00fchn U, Wahle E (2004) Structure and function of poly(A) binding proteins. Biochim Biophys Acta 1678: 67\u201384","journal-title":"Biochim Biophys Acta"},{"key":"BFEMBR202256327_CR28","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1073\/pnas.84.7.1926","volume":"84","author":"AD Kwong","year":"1987","unstructured":"Kwong AD, Frenkel N (1987) Herpes simplex virus\u2010infected cells contain a function(s) that destabilizes both host and viral mRNAs. Proc Natl Acad Sci USA 84: 1926\u20131930","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBR202256327_CR29","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1093\/bioinformatics\/bty191","volume":"34","author":"H Li","year":"2018","unstructured":"Li H (2018) Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics 34: 3094\u20133100","journal-title":"Bioinformatics"},{"key":"BFEMBR202256327_CR30","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R (2009) The Sequence Alignment\/Map format and SAMtools. Bioinformatics 25: 2078\u20132079","journal-title":"Bioinformatics"},{"key":"BFEMBR202256327_CR31","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1126\/science.aam5794","volume":"361","author":"J Lim","year":"2018","unstructured":"Lim J, Kim D, Lee YS, Ha M, Lee M, Yeo J, Chang H, Song J, Ahn K, Kim VN (2018) Mixed tailing by TENT4A and TENT4B shields mRNA from rapid deadenylation. Science 361: 701\u2013704","journal-title":"Science"},{"key":"BFEMBR202256327_CR32","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real\u2010time quantitative PCR and the 2(\u2010Delta Delta C(T)) Method. Methods 25: 402\u2013408","journal-title":"Methods"},{"key":"BFEMBR202256327_CR33","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.59186","volume":"9","author":"S Mattijssen","year":"2020","unstructured":"Mattijssen S, Iben JR, Li T, Coon SL, Maraia RJ (2020) Single molecule poly(A) tail\u2010seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails. Elife 9: e59186","journal-title":"Elife"},{"key":"BFEMBR202256327_CR34","doi-asserted-by":"crossref","first-page":"5627","DOI":"10.1073\/pnas.1202829109","volume":"109","author":"C McKinney","year":"2012","unstructured":"McKinney C, Perez C, Mohr I (2012) Poly(A) binding protein abundance regulates eukaryotic translation initiation factor 4F assembly in human\u00a0cytomegalovirus\u2010infected cells. Proc Natl Acad Sci USA 109: 5627\u20135632","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBR202256327_CR35","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.celrep.2013.11.045","volume":"6","author":"C McKinney","year":"2014","unstructured":"McKinney C, Zavadil J, Bianco C, Shiflett L, Brown S, Mohr I (2014) Global reprogramming of the cellular translational landscape facilitates cytomegalovirus replication. Cell Rep 6: 9\u201317","journal-title":"Cell Rep"},{"key":"BFEMBR202256327_CR36","doi-asserted-by":"crossref","first-page":"4678","DOI":"10.1038\/emboj.2011.320","volume":"30","author":"M Morita","year":"2011","unstructured":"Morita M, Oike Y, Nagashima T, Kadomatsu T, Tabata M, Suzuki T, Nakamura T, Yoshida N, Okada M, Yamamoto T (2011) Obesity resistance and increased hepatic expression of catabolism\u2010related mRNAs in Cnot3+\/\u2212 mice. EMBO J 30: 4678\u20134691","journal-title":"EMBO J"},{"key":"BFEMBR202256327_CR37","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.49894","volume":"8","author":"LV Nobre","year":"2019","unstructured":"Nobre LV, Nightingale K, Ravenhill BJ, Antrobus R, Soday L, Nichols J, Davies JA, Seirafian S, Wang EC, Davison AJ et\u00a0al (2019) Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. Elife 8: e49894","journal-title":"Elife"},{"key":"BFEMBR202256327_CR38","doi-asserted-by":"crossref","first-page":"5527","DOI":"10.1128\/JVI.02047-07","volume":"82","author":"TJ Pasieka","year":"2008","unstructured":"Pasieka TJ, Lu B, Crosby SD, Wylie KM, Morrison LA, Alexander DE, Menachery VD, Leib DA (2008) Herpes simplex virus virion host shutoff attenuates establishment of the antiviral state. J Virol 82: 5527\u20135535","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR39","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/s41580-021-00417-y","volume":"23","author":"LA Passmore","year":"2022","unstructured":"Passmore LA, Coller J (2022) Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression. Nat Rev Mol Cell Biol 23: 93\u2013106","journal-title":"Nat Rev Mol Cell Biol"},{"key":"BFEMBR202256327_CR40","doi-asserted-by":"crossref","DOI":"10.3390\/v13050817","volume":"13","author":"MR Perera","year":"2021","unstructured":"Perera MR, Wills MR, Sinclair JH (2021) HCMV antivirals and strategies to target the latent reservoir. Viruses 13: 817","journal-title":"Viruses"},{"key":"BFEMBR202256327_CR41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1128\/JVI.01778-10","volume":"85","author":"C Perez","year":"2011","unstructured":"Perez C, McKinney C, Chulunbaatar U, Mohr I (2011) Translational control of the abundance of cytoplasmic poly(A) binding protein in human cytomegalovirus\u2010infected cells. J Virol 85: 156\u2013164","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR42","doi-asserted-by":"crossref","first-page":"11.12.11","DOI":"10.1002\/0471250953.bi1112s47","volume":"47","author":"AR Quinlan","year":"2014","unstructured":"Quinlan AR (2014) BEDTools: the Swiss\u2010Army Tool for genome feature analysis. Curr Protoc Bioinformatics 47: 11.12.11\u201311.12.34","journal-title":"Curr Protoc Bioinformatics"},{"key":"BFEMBR202256327_CR43","doi-asserted-by":"crossref","first-page":"3173","DOI":"10.1038\/s41467-019-11094-z","volume":"10","author":"T Raisch","year":"2019","unstructured":"Raisch T, Chang CT, Levdansky Y, Muthukumar S, Raunser S, Valkov E (2019) Reconstitution of recombinant human CCR4\u2010NOT reveals molecular insights into regulated deadenylation. Nat Commun 10: 3173","journal-title":"Nat Commun"},{"key":"BFEMBR202256327_CR44","doi-asserted-by":"crossref","first-page":"10763","DOI":"10.1128\/JVI.01197-13","volume":"87","author":"JM Reitsma","year":"2013","unstructured":"Reitsma JM, Sato H, Nevels M, Terhune SS, Paulus C (2013) Human cytomegalovirus IE1 protein disrupts interleukin\u20106 signaling by sequestering STAT3 in the nucleus. J Virol 87: 10763\u201310776","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR45","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1101\/gad.319475.118","volume":"32","author":"RM Rubio","year":"2018","unstructured":"Rubio RM, Depledge DP, Bianco C, Thompson L, Mohr I (2018) RNA m(6) A modification enzymes shape innate responses to DNA by regulating interferon beta. Genes Dev 32: 1472\u20131484","journal-title":"Genes Dev"},{"key":"BFEMBR202256327_CR46","doi-asserted-by":"crossref","first-page":"7126","DOI":"10.1038\/ncomms8126","volume":"6","author":"AJ Rutkowski","year":"2015","unstructured":"Rutkowski AJ, Erhard F, L'Hernault A, Bonfert T, Schilhabel M, Crump C, Rosenstiel P, Efstathiou S, Zimmer R, Friedel CC et\u00a0al (2015) Widespread disruption of host transcription termination in HSV\u20101 infection. Nat Commun 6: 7126","journal-title":"Nat Commun"},{"key":"BFEMBR202256327_CR47","volume":"9","author":"S Scarpini","year":"2021","unstructured":"Scarpini S, Morigi F, Betti L, Dondi A, Biagi C, Lanari M (2021) Development of a vaccine against human cytomegalovirus: advances, barriers, and implications for the clinical practice. Vaccines (Basel) 9: 551","journal-title":"Vaccines (Basel)"},{"key":"BFEMBR202256327_CR48","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1261\/rna.079050.121","volume":"28","author":"J Shin","year":"2022","unstructured":"Shin J, Paek KY, Chikhaoui L, Jung S, Ponny S, Suzuki Y, Padmanabhan K, Richter JD (2022) Oppositional poly(A) tail length regulation by FMRP and CPEB1. RNA 28: 756\u2013765","journal-title":"RNA"},{"key":"BFEMBR202256327_CR49","doi-asserted-by":"crossref","first-page":"18619","DOI":"10.1073\/pnas.1909314116","volume":"116","author":"J Song","year":"2019","unstructured":"Song J, Lee S, Cho DY, Lee S, Kim H, Yu N, Lee S, Ahn K (2019) Human cytomegalovirus induces and exploits Roquin to counteract the IRF1\u2010mediated antiviral state. Proc Natl Acad Sci USA 116: 18619\u201318628","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBR202256327_CR50","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2022.167523","volume":"434","author":"PN Stoney","year":"2022","unstructured":"Stoney PN, Yanagiya A, Nishijima S, Yamamoto T (2022) CNOT7 outcompetes its paralog CNOT8 for integration into the CCR4\u2010NOT complex. J Mol Biol 434: 167523","journal-title":"J Mol Biol"},{"key":"BFEMBR202256327_CR51","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008390","volume":"16","author":"J Tai\u2010Schmiedel","year":"2020","unstructured":"Tai\u2010Schmiedel J, Karniely S, Lau B, Ezra A, Eliyahu E, Nachshon A, Kerr K, Su\u00e1rez N, Schwartz M, Davison AJ et\u00a0al (2020) Human cytomegalovirus long noncoding RNA4.9 regulates viral DNA replication. PLoS Pathog 16: e1008390","journal-title":"PLoS Pathog"},{"key":"BFEMBR202256327_CR52","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1128\/JVI.02124-06","volume":"81","author":"S Terhune","year":"2007","unstructured":"Terhune S, Torigoi E, Moorman N, Silva M, Qian Z, Shenk T, Yu D (2007) Human cytomegalovirus UL38 protein blocks apoptosis. J Virol 81: 3109\u20133123","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR53","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2022.110767","volume":"39","author":"L Thompson","year":"2022","unstructured":"Thompson L, Depledge DP, Burgess HM, Mohr I (2022) An eIF3d\u2010dependent switch regulates HCMV replication by remodeling the infected cell\u00a0translation landscape to mimic chronic ER stress. Cell Rep 39: 110767","journal-title":"Cell Rep"},{"key":"BFEMBR202256327_CR54","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005288","volume":"11","author":"O Tirosh","year":"2015","unstructured":"Tirosh O, Cohen Y, Shitrit A, Shani O, Le\u2010Trilling VTK, Trilling M, Friedlander G, Tanenbaum M, Stern\u2010Ginossar N (2015) The transcription and translation landscapes during human cytomegalovirus infection reveal novel host\u2010pathogen interactions. PLoS Pathog 11: e1005288","journal-title":"PLoS Pathog"},{"key":"BFEMBR202256327_CR55","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002444","volume":"7","author":"M Umashankar","year":"2011","unstructured":"Umashankar M, Petrucelli A, Cicchini L, Caposio P, Kreklywich CN, Rak M, Bughio F, Goldman DC, Hamlin KL, Nelson JA et\u00a0al (2011) A novel human cytomegalovirus locus modulates cell type\u2010specific outcomes of infection. PLoS Pathog 7: e1002444","journal-title":"PLoS Pathog"},{"key":"BFEMBR202256327_CR56","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/ni.2366","volume":"13","author":"DA Vignali","year":"2012","unstructured":"Vignali DA, Kuchroo VK (2012) IL\u201012 family cytokines: immunological playmakers. Nat Immunol 13: 722\u2013728","journal-title":"Nat Immunol"},{"key":"BFEMBR202256327_CR67","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.cell.2012.08.004","volume":"150","author":"A Wagschal","year":"2012","unstructured":"Wagschal A, Rousset E, Basavarajaiah P, Contreras X, Harwig A, Laurent-Chabalier S, Nakamura M, Chen X, Zhang K, Meziane O et\u00a0al (2012) Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII. Cell 150: 1147\u20131157","journal-title":"Cell"},{"key":"BFEMBR202256327_CR57","doi-asserted-by":"crossref","first-page":"8057","DOI":"10.1128\/JVI.79.13.8057-8064.2005","volume":"79","author":"D Walsh","year":"2005","unstructured":"Walsh D, Perez C, Notary J, Mohr I (2005) Regulation of the translation initiation factor eIF4F by multiple mechanisms in human cytomegalovirus\u2010infected cells. J Virol 79: 8057\u20138064","journal-title":"J Virol"},{"key":"BFEMBR202256327_CR58","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1016\/j.cell.2014.04.028","volume":"157","author":"MP Weekes","year":"2014","unstructured":"Weekes MP, Tomasec P, Huttlin EL, Fielding CA, Nusinow D, Stanton RJ, Wang ECY, Aicheler R, Murrell I, Wilkinson GWG et\u00a0al (2014) Quantitative temporal viromics: an approach to investigate host\u2010pathogen interaction. Cell 157: 1460\u20131472","journal-title":"Cell"},{"key":"BFEMBR202256327_CR59","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nature03035","volume":"432","author":"S West","year":"2004","unstructured":"West S, Gromak N, Proudfoot NJ (2004) Human 5\u2032 \u2192 3\u2032 exonuclease Xrn2 promotes transcription termination at co\u2010transcriptional cleavage sites. Nature 432: 522\u2013525","journal-title":"Nature"},{"key":"BFEMBR202256327_CR60","doi-asserted-by":"crossref","DOI":"10.1007\/978-0-387-98141-3","volume-title":"ggplot2: elegant graphics for data analysis","author":"H Wickham","year":"2009","unstructured":"Wickham H (2009) ggplot2: elegant graphics for data analysis. New York, NY: Springer"},{"key":"BFEMBR202256327_CR61","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00430-015-0411-4","volume":"204","author":"GW Wilkinson","year":"2015","unstructured":"Wilkinson GW, Davison AJ, Tomasec P, Fielding CA, Aicheler R, Murrell I, Seirafian S, Wang EC, Weekes M, Lehner PJ et\u00a0al (2015) Human cytomegalovirus: taking the strain. Med Microbiol Immunol 204: 273\u2013284","journal-title":"Med Microbiol Immunol"},{"key":"BFEMBR202256327_CR62","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1038\/s41592-019-0617-2","volume":"16","author":"RE Workman","year":"2019","unstructured":"Workman RE, Tang AD, Tang PS, Jain M, Tyson JR, Razaghi R, Zuzarte PC, Gilpatrick T, Payne A, Quick J et\u00a0al (2019) Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat Methods 16: 1297\u20131305","journal-title":"Nat Methods"},{"key":"BFEMBR202256327_CR63","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1016\/S0006-291X(02)00562-4","volume":"294","author":"Z Yang","year":"2002","unstructured":"Yang Z, Wara\u2010aswapati N, Yoshida Y, Walker N, Galson DL, Listman J, Auron PE (2002) Dual regulatory role of human cytomegalovirus immediate\u2010early protein in IL1B transcription is dependent upon Spi\u20101\/PU.1. Biochem Biophys Res Commun 294: 854\u2013863","journal-title":"Biochem Biophys Res Commun"},{"key":"BFEMBR202256327_CR64","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1016\/j.molcel.2018.05.009","volume":"70","author":"H Yi","year":"2018","unstructured":"Yi H, Park J, Ha M, Lim J, Chang H, Kim VN (2018) PABP cooperates with the CCR4\u2010NOT complex to promote mRNA deadenylation and block precocious decay. Mol Cell 70: 1081\u20131088.e5","journal-title":"Mol Cell"},{"key":"BFEMBR202256327_CR65","volume":"9","author":"ZJ Zhang","year":"2020","unstructured":"Zhang ZJ, Gao Q, Fang XD, Ding ZH, Gao DM, Xu WY, Cao Q, Qiao JH, Yang YZ, Han C et\u00a0al (2020) CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication. eLife 9: e53753","journal-title":"eLife"}],"container-title":["The EMBO Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embr.202256327.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/embr.202256327\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/embr.202256327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/embr.202256327.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:10:32Z","timestamp":1779113432000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/embr.202256327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,17]]},"references-count":66,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12,6]]}},"alternative-id":["BFEMBR202256327"],"URL":"https:\/\/doi.org\/10.15252\/embr.202256327","relation":{},"ISSN":["1469-221X","1469-3178"],"issn-type":[{"value":"1469-221X","type":"print"},{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,17]]},"assertion":[{"value":"18 October 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 September 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 September 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 October 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"EMBR202256327"}}