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In recent years, new methods to expand epithelial cells as three\u2010dimensional organoids have provided novel means to investigate the functions of these cells in health and disease. Here, we outline the history of stratified epithelial stem cell culture and the application of cultured epithelial cells in clinical therapies. We further discuss the derivation of organoids from other types of epithelia and the challenges that remain for the translation of novel stem cell therapies toward clinical use.\n                  <\/jats:p>","DOI":"10.15252\/emmm.201708213","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T20:11:08Z","timestamp":1514578268000},"page":"139-150","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-8791","authenticated-orcid":false,"given":"Robert E","family":"Hynds","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9655-3766","authenticated-orcid":false,"given":"Paola","family":"Bonfanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-5939","authenticated-orcid":false,"given":"Sam M","family":"Janes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,12,29]]},"reference":[{"key":"BFEMMM201708213_CR1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1002\/jcp.1040720208","volume":"72","author":"SA Aaronson","year":"1968","unstructured":"Aaronson SA, Todaro GJ (1968) Development of 3T3\u2010like lines from Balb\/C mouse embryo cultures \u2013 transformation susceptibility to Sv40. 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