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However, a limited number of organ donors and insufficient vascularization with islet engraftment failure restrict the successful transfer of this approach into clinical practice. To overcome these problems, we herein introduce a novel strategy for the generation of prevascularized islet organoids by the fusion of pancreatic islet cells with functional native microvessels. These insulin\u2010secreting organoids exhibit a significantly higher angiogenic activity compared to freshly isolated islets, cultured islets, and non\u2010prevascularized islet organoids. This is caused by paracrine signaling between the \u03b2\u2010cells and the microvessels, mediated by insulin binding to its corresponding receptor on endothelial cells.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , the prevascularized islet organoids are rapidly blood\u2010perfused after transplantation by the interconnection of their autochthonous microvasculature with surrounding blood vessels. As a consequence, a lower number of islet grafts are required to restore normoglycemia in diabetic mice. Thus, prevascularized islet organoids may be used to improve the success rates of clinical islet transplantation.\n                  <\/jats:p>","DOI":"10.15252\/emmm.202012616","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T05:04:16Z","timestamp":1604293456000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["Improvement of islet transplantation by the fusion of islet cells with functional blood vessels"],"prefix":"10.1038","volume":"13","author":[{"given":"Lisa","family":"Nalbach","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia P","family":"Roma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate M","family":"Schmitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivien","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"K\u00f6rbel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selina","family":"Wrublewsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy","family":"Pack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Sp\u00e4ter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Metzger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian M","family":"Menger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian S","family":"Frueh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"G\u00f6tz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haopeng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn E","family":"Manning Fox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick E","family":"MacDonald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D","family":"Menger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7847-8456","authenticated-orcid":false,"given":"Matthias W","family":"Laschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1886-5657","authenticated-orcid":false,"given":"Emmanuel","family":"Ampofo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"BFEMMM202012616_CR1","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1016\/j.bbadis.2015.07.013","volume":"1852","author":"E Ampofo","year":"2015","unstructured":"Ampofo E, Rudzitis\u2010Auth J, Dahmke IN, Roessler OG, Thiel G, Montenarh M, Menger MD, Laschke MW (2015) Inhibition of protein kinase CK2 suppresses tumor necrosis factor (TNF)\u2010alpha\u2010induced leukocyte\u2010endothelial cell interaction. 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