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Ltd."}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Mol Med"],"published-print":{"date-parts":[[2021,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The genus Flavivirus comprises numerous emerging and re\u2010emerging arboviruses causing human illness. Vaccines are the best approach to prevent flavivirus diseases. But pathogen diversities are always one of the major hindrances for timely development of new vaccines when confronting unpredicted flavivirus outbreaks. We used West Nile virus (WNV) as a model to develop a new live\u2010attenuated vaccine (LAV), WNV\u2010poly(A), by replacing 5\u02b9 portion (corresponding to SL and DB domains in WNV) of 3\u02b9\u2010UTR with internal poly(A) tract. WNV\u2010poly(A) not only propagated efficiently in Vero cells, but also was highly attenuated in mouse model. A single\u2010dose vaccination elicited robust and long\u2010lasting immune responses, conferring full protection against WNV challenge. Such \u201cpoly(A)\u201d vaccine strategy may be promising for wide application in the development of flavivirus LAVs because of its general target regions in flaviviruses.<\/jats:p>","DOI":"10.15252\/emmm.202114108","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T10:08:49Z","timestamp":1628158129000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Rational design of West Nile virus vaccine through large replacement of 3\u2032 UTR with internal poly(A)"],"prefix":"10.1038","volume":"13","author":[{"given":"Ya\u2010Nan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu\u2010Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun\u2010Li","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010Yue","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Qing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4953-6709","authenticated-orcid":false,"given":"Xiao\u2010Dan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Lin","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Yong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Ming","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao\u2010Peng","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7770-4071","authenticated-orcid":false,"given":"Han\u2010Qing","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8895-3679","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"BFEMMM202114108_CR1","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1001\/jamaneurol.2016.0800","volume":"73","author":"JD Beckham","year":"2016","unstructured":"Beckham JD, Pastula DM, Massey A, Tyler KL (2016) Zika virus as an emerging global pathogen: neurological complications of Zika virus. 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