{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:49:30Z","timestamp":1792802970779,"version":"4.5.0"},"reference-count":55,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62175031"],"award-info":[{"award-number":["62175031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61727823"],"award-info":[{"award-number":["61727823"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A201252"],"award-info":[{"award-number":["U22A201252"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013092","name":"Science and Technology Planning Project of Fuzhou","doi-asserted-by":"publisher","award":["2021\u2010R\u2010105"],"award-info":[{"award-number":["2021\u2010R\u2010105"]}],"id":[{"id":"10.13039\/501100013092","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013092","name":"Science and Technology Planning Project of Fuzhou","doi-asserted-by":"publisher","award":["2021\u2010S\u2010wt3"],"award-info":[{"award-number":["2021\u2010S\u2010wt3"]}],"id":[{"id":"10.13039\/501100013092","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017357","name":"Science and Technology Projects of Fujian Province","doi-asserted-by":"publisher","award":["2022J011282"],"award-info":[{"award-number":["2022J011282"]}],"id":[{"id":"10.13039\/100017357","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientic Foundation of Fuzhou Municipal Health Commission","award":["2022\u2010S\u2010rc1"],"award-info":[{"award-number":["2022\u2010S\u2010rc1"]}],"id":[{"id":"https:\/\/ror.org\/025qxt858","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Mol Med"],"published-print":{"date-parts":[[2023,10,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neoantigens are emerging as attractive targets to develop personalized cancer vaccines, but their immunization efficacy is severely hampered by their restricted accessibility to lymphoid tissues where immune responses are initiated. Leveraging the capability of red blood cells (RBCs) to capture and present pathogens in peripheral blood to the antigen\u2010presenting cells (APCs) in spleen, we developed a RBC\u2010driven spleen targeting strategy to deliver DNA vaccine encoding hepatocellular carcinoma (HCC) neoantigen. The DNA vaccine\u2010encapsulating polymeric nanoparticles that were intentionally hitchhiked on the preisolated RBCs could preferentially accumulate in the spleen to promote the neoantigen expression by APCs, resulting in the burst of neoantigen\u2010specific T\u2010cell immunity to prevent tumorigenesis in a personalized manner, and slow down tumor growth in the established aggressively growing HCC. Remarkably, when combined with anti\u2010PD\u20101, the vaccine achieved complete tumor regression and generated a robust systemic immune response with long\u2010term tumor\u2010specific immunological memory, which thoroughly prevented tumor recurrence and spontaneous lung metastasis. This study offers a prospective strategy to develop personalized neoantigen vaccines for augmenting cancer immunotherapy efficiency in immune \u201ccold\u201d HCC.<\/jats:p>","DOI":"10.15252\/emmm.202216836","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T11:16:26Z","timestamp":1691493386000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Spleen\u2010targeted neoantigen DNA vaccine for\u00a0personalized immunotherapy of hepatocellular\u00a0carcinoma"],"prefix":"10.1038","volume":"15","author":[{"given":"Ming","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5717-0997","authenticated-orcid":false,"given":"Zijin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0912-8372","authenticated-orcid":false,"given":"Zhixiong","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenli","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7036-5783","authenticated-orcid":false,"given":"Aoxue","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5166-0223","authenticated-orcid":false,"given":"Liming","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3096-4981","authenticated-orcid":false,"given":"Xiaolong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"BFEMMM202216836_CR1","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.abc6659","volume":"12","author":"S Atasheva","year":"2020","unstructured":"Atasheva S, Emerson CC, Yao J, Young C, Stewart PL, Shayakhmetov DM (2020) Systemic cancer therapy with engineered adenovirus that evades innate immunity. Sci Transl Med 12: eabc6659","journal-title":"Sci Transl Med"},{"key":"BFEMMM202216836_CR2","doi-asserted-by":"crossref","first-page":"8368","DOI":"10.1158\/0008-5472.CAN-10-1322","volume":"70","author":"R Bos","year":"2010","unstructured":"Bos R, Sherman LA (2010) CD4+ T\u2010cell help in the tumor milieu is required for recruitment and cytolytic function of CD8+ T lymphocytes. Cancer Res 70: 8368\u20138377","journal-title":"Cancer Res"},{"key":"BFEMMM202216836_CR3","doi-asserted-by":"crossref","first-page":"2684","DOI":"10.1038\/s41467-018-05079-7","volume":"9","author":"JS Brenner","year":"2018","unstructured":"Brenner JS, Pan DC, Myerson JW, Marcos\u2010Contreras OA, Villa CH, Patel P, Hekierski H, Chatterjee S, Tao J\u2010Q, Parhiz H et\u00a0al (2018) Red blood cell\u2010hitchhiking boosts delivery of nanocarriers to chosen organs by orders of magnitude. Nat Commun 9: 2684","journal-title":"Nat Commun"},{"key":"BFEMMM202216836_CR4","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12943-021-01467-8","volume":"20","author":"Z Cai","year":"2021","unstructured":"Cai Z, Su X, Qiu L, Li Z, Li X, Dong X, Wei F, Zhou Y, Luo L, Chen G et\u00a0al (2021) Personalized neoantigen vaccine prevents postoperative recurrence in hepatocellular carcinoma patients with vascular invasion. Mol Cancer 20: 164","journal-title":"Mol Cancer"},{"key":"BFEMMM202216836_CR5","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1126\/science.aaa3828","volume":"348","author":"BM Carreno","year":"2015","unstructured":"Carreno BM, Magrini V, Becker\u2010Hapak M, Kaabinejadian S, Hundal J, Petti AA, Ly A, Lie W\u2010R, Hildebrand WH, Mardis ER et\u00a0al (2015) A dendritic cell vaccine increases the breadth and diversity of melanoma neoantigen\u2010specific T cells. Science 348: 803\u2013808","journal-title":"Science"},{"key":"BFEMMM202216836_CR6","volume":"13","author":"L Chen","year":"2021","unstructured":"Chen L, Qin H, Zhao R, Zhao X, Lin L, Chen Y, Lin Y, Li Y, Qin Y, Li Y et\u00a0al (2021) Bacterial cytoplasmic membranes synergistically enhance the antitumor activity of autologous cancer vaccines. Sci Transl Med 13: eabc2816","journal-title":"Sci Transl Med"},{"key":"BFEMMM202216836_CR7","volume":"10","author":"H Chen","year":"2022","unstructured":"Chen H, Li Z, Qiu L, Dong X, Chen G, Shi Y, Cai L, Liu W, Ye H, Zhou Y et\u00a0al (2022) Personalized neoantigen vaccine combined with PD\u20101 blockade increases CD8+ tissue\u2010resident memory T\u2010cell infiltration in preclinical hepatocellular carcinoma models. J Immunother Cancer 10: e004389","journal-title":"J Immunother Cancer"},{"key":"BFEMMM202216836_CR8","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/s41565-020-0669-6","volume":"15","author":"Q Cheng","year":"2020","unstructured":"Cheng Q, Wei T, Farbiak L, Johnson LT, Dilliard SA, Siegwart DJ (2020) Selective organ targeting (SORT) nanoparticles for tissue\u2010specific mRNA delivery and CRISPR\u2013Cas gene editing. Nat Nanotechnol 15: 313\u2013320","journal-title":"Nat Nanotechnol"},{"key":"BFEMMM202216836_CR9","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1186\/s40425-019-0768-9","volume":"7","author":"AA Davis","year":"2019","unstructured":"Davis AA, Patel VG (2019) The role of PD\u2010L1 expression as a predictive biomarker: an analysis of all US Food and Drug Administration (FDA) approvals of immune checkpoint inhibitors. J Immunother Cancer 7: 278","journal-title":"J Immunother Cancer"},{"key":"BFEMMM202216836_CR10","doi-asserted-by":"crossref","first-page":"223","DOI":"10.4049\/jimmunol.1800933","volume":"205","author":"P Deb","year":"2020","unstructured":"Deb P, Dai J, Singh S, Kalyoussef E, Fitzgerald\u2010Bocarsly P (2020) Triggering of the cGAS\u2013STING pathway in human plasmacytoid dendritic cells inhibits TLR9\u2010mediated IFN production. J Immunol 205: 223\u2013236","journal-title":"J Immunol"},{"key":"BFEMMM202216836_CR11","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","volume":"29","author":"A Dobin","year":"2012","unstructured":"Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR (2012) STAR: ultrafast universal RNA\u2010seq aligner. Bioinformatics 29: 15\u201321","journal-title":"Bioinformatics"},{"key":"BFEMMM202216836_CR12","doi-asserted-by":"crossref","first-page":"4666","DOI":"10.1021\/acsnano.1c11374","volume":"16","author":"LT Ferguson","year":"2022","unstructured":"Ferguson LT, Hood ED, Shuvaeva T, Shuvaev VV, Basil MC, Wang Z, Nong J, Ma X, Wu J, Myerson JW et\u00a0al (2022) Dual affinity to RBCs and target cells (DART) enhances both organ\u2010 and cell type\u2010targeting of intravascular nanocarriers. ACS Nano 16: 4666\u20134683","journal-title":"ACS Nano"},{"key":"BFEMMM202216836_CR13","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/j.celrep.2017.04.031","volume":"19","author":"A Garcia\u2010Diaz","year":"2017","unstructured":"Garcia\u2010Diaz A, Shin DS, Moreno BH, Saco J, Escuin\u2010Ordinas H, Rodriguez GA, Zaretsky JM, Sun L, Hugo W, Wang X et\u00a0al (2017) Interferon receptor signaling pathways regulating PD\u2010L1 and PD\u2010L2 expression. Cell Rep 19: 1189\u20131201","journal-title":"Cell Rep"},{"key":"BFEMMM202216836_CR14","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.41090","volume":"7","author":"RS Gejman","year":"2018","unstructured":"Gejman RS, Chang AY, Jones HF, DiKun K, Hakimi AA, Schietinger A, Scheinberg DA (2018) Rejection of immunogenic tumor clones is limited by clonal fraction. Elife 7: e41090","journal-title":"Elife"},{"key":"BFEMMM202216836_CR15","volume":"5","author":"X Han","year":"2019","unstructured":"Han X, Shen S, Fan Q, Chen G, Archibong E, Dotti G, Liu Z, Gu Z, Wang C (2019) Red blood cell\u2010derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy. Sci Adv 5: eaaw6870","journal-title":"Sci Adv"},{"key":"BFEMMM202216836_CR16","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/nri.2017.131","volume":"18","author":"Z Hu","year":"2018","unstructured":"Hu Z, Ott PA, Wu CJ (2018) Towards personalized, tumour\u2010specific, therapeutic vaccines for cancer. Nat Rev Immunol 18: 168\u2013182","journal-title":"Nat Rev Immunol"},{"key":"BFEMMM202216836_CR17","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/s41551-018-0284-0","volume":"2","author":"MA Islam","year":"2018","unstructured":"Islam MA, Xu Y, Tao W, Ubellacker JM, Lim M, Aum D, Lee GY, Zhou K, Zope H, Yu M et\u00a0al (2018) Restoration of tumour\u2010growth suppression in vivo via systemic nanoparticle\u2010mediated delivery of PTEN mRNA. Nat Biomed Eng 2: 850\u2013864","journal-title":"Nat Biomed Eng"},{"key":"BFEMMM202216836_CR18","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/ni957","volume":"4","author":"AA Itano","year":"2003","unstructured":"Itano AA, Jenkins MK (2003) Antigen presentation to naive CD4 T cells in the lymph node. Nat Immunol 4: 733\u2013739","journal-title":"Nat Immunol"},{"key":"BFEMMM202216836_CR19","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12943-018-0928-4","volume":"18","author":"X Jiang","year":"2019","unstructured":"Jiang X, Wang J, Deng X, Xiong F, Ge J, Xiang B, Wu X, Ma J, Zhou M, Li X et\u00a0al (2019) Role of the tumor microenvironment in PD\u2010L1\/PD\u20101\u2010mediated tumor immune escape. Mol Cancer 18: 10","journal-title":"Mol Cancer"},{"key":"BFEMMM202216836_CR20","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1038\/s41565-017-0043-5","volume":"13","author":"R Kedmi","year":"2018","unstructured":"Kedmi R, Veiga N, Ramishetti S, Goldsmith M, Rosenblum D, Dammes N, Hazan\u2010Halevy I, Nahary L, Leviatan\u2010Ben\u2010Arye S, Harlev M et\u00a0al (2018) A modular platform for targeted RNAi therapeutics. Nat Nanotechnol 13: 214\u2013219","journal-title":"Nat Nanotechnol"},{"key":"BFEMMM202216836_CR21","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1056\/NEJMe2004851","volume":"382","author":"RK Kelley","year":"2020","unstructured":"Kelley RK (2020) Atezolizumab plus Bevacizumab \u2014 a landmark in liver cancer. N Engl J Med 382: 1953\u20131955","journal-title":"N Engl J Med"},{"key":"BFEMMM202216836_CR22","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/nature18300","volume":"534","author":"LM Kranz","year":"2016","unstructured":"Kranz LM, Diken M, Haas H, Kreiter S, Loquai C, Reuter KC, Meng M, Fritz D, Vascotto F, Hefesha H et\u00a0al (2016) Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy. Nature 534: 396\u2013401","journal-title":"Nature"},{"key":"BFEMMM202216836_CR23","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1038\/s41551-020-00623-7","volume":"4","author":"M Krohn\u2010Grimberghe","year":"2020","unstructured":"Krohn\u2010Grimberghe M, Mitchell MJ, Schloss MJ, Khan OF, Courties G, Guimaraes PPG, Rohde D, Cremer S, Kowalski PS, Sun Y et\u00a0al (2020) Nanoparticle\u2010encapsulated siRNAs for gene silencing in the haematopoietic stem\u2010cell niche. Nat Biomed Eng 4: 1076\u20131089","journal-title":"Nat Biomed Eng"},{"key":"BFEMMM202216836_CR24","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/nmat4822","volume":"16","author":"R Kuai","year":"2017","unstructured":"Kuai R, Ochyl LJ, Bahjat KS, Schwendeman A, Moon JJ (2017) Designer vaccine nanodiscs for personalized cancer immunotherapy. Nat Mater 16: 489\u2013496","journal-title":"Nat Mater"},{"key":"BFEMMM202216836_CR25","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/s13073-021-00872-4","volume":"13","author":"L Li","year":"2021","unstructured":"Li L, Zhang X, Wang X, Kim SW, Herndon JM, Becker\u2010Hapak MK, Carreno BM, Myers NB, Sturmoski MA, McLellan MD et\u00a0al (2021) Optimized polyepitope neoantigen DNA vaccines elicit neoantigen\u2010specific immune responses in preclinical models and in clinical translation. Genome Med 13: 56","journal-title":"Genome Med"},{"key":"BFEMMM202216836_CR26","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.mpsur.2010.01.004","volume":"28","author":"DM Lloyd","year":"2010","unstructured":"Lloyd DM, Strickland AD (2010) Surgery of the spleen. Surgery (Oxford) 28: 229\u2013233","journal-title":"Surgery (Oxford)"},{"key":"BFEMMM202216836_CR27","first-page":"1071","volume":"59","author":"AL Marzo","year":"1999","unstructured":"Marzo AL, Lake RA, Robinson BWS, Scott B (1999) T\u2010cell receptor transgenic analysis of tumor\u2010specific CD8 and CD4 responses in the eradication of solid tumors. Cancer Res 59: 1071\u20131079","journal-title":"Cancer Res"},{"key":"BFEMMM202216836_CR28","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s12026-018-8988-5","volume":"66","author":"H Minasyan","year":"2018","unstructured":"Minasyan H (2018) Phagocytosis and oxycytosis: two arms of human innate immunity. Immunol Res 66: 271\u2013280","journal-title":"Immunol Res"},{"key":"BFEMMM202216836_CR29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jconrel.2022.01.020","volume":"343","author":"T Nakamura","year":"2022","unstructured":"Nakamura T, Haloho SEE, Harashima H (2022) Intravenous liposomal vaccine enhances CTL generation, but not until antigen presentation. J Control Release 343: 1\u201312","journal-title":"J Control Release"},{"key":"BFEMMM202216836_CR30","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nmeth.3337","volume":"12","author":"AM Newman","year":"2015","unstructured":"Newman AM, Liu CL, Green MR, Gentles AJ, Feng W, Xu Y, Hoang CD, Diehn M, Alizadeh AA (2015) Robust enumeration of cell subsets from tissue expression profiles. Nat Methods 12: 453\u2013457","journal-title":"Nat Methods"},{"key":"BFEMMM202216836_CR31","doi-asserted-by":"crossref","first-page":"11623","DOI":"10.1021\/acsnano.0c04188","volume":"14","author":"TL Nguyen","year":"2020","unstructured":"Nguyen TL, Yin Y, Choi Y, Jeong JH, Kim J (2020) Enhanced cancer DNA vaccine via direct transfection to host dendritic cells recruited in injectable scaffolds. ACS Nano 14: 11623\u201311636","journal-title":"ACS Nano"},{"key":"BFEMMM202216836_CR32","first-page":"A723","volume":"10","author":"R Perales","year":"2022","unstructured":"Perales R, Perales\u2010Puchalt A, Bartha G, Northcott J, Chen R, Lyle J, Norton D, Cooch N, Gane E, Yarchoan M et\u00a0al (2022) 692 Circulating tumor DNA analysis of advanced hepatocellular cancer (HCC) patients treated with neoantigen targeted personalized cancer DNA vaccine (GNOS\u2010PV02) in combination with plasmid IL\u201012 (pIL12) and anti\u2010PD1 (pembrolizumab). J\u00a0Immunother Cancer 10: A723","journal-title":"J\u00a0Immunother Cancer"},{"key":"BFEMMM202216836_CR33","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s12026-009-8113-x","volume":"45","author":"AH Rahman","year":"2009","unstructured":"Rahman AH, Taylor DK, Turka LA (2009) The contribution of direct TLR signaling to T cell responses. Immunol Res 45: 25\u201336","journal-title":"Immunol Res"},{"key":"BFEMMM202216836_CR34","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/nrc2326","volume":"8","author":"J Rice","year":"2008","unstructured":"Rice J, Ottensmeier CH, Stevenson FK (2008) DNA vaccines: precision tools for activating effective immunity against cancer. Nat Rev Cancer 8: 108\u2013120","journal-title":"Nat Rev Cancer"},{"key":"BFEMMM202216836_CR35","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkv007","volume":"43","author":"ME Ritchie","year":"2015","unstructured":"Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W, Smyth GK (2015) limma powers differential expression analyses for RNA\u2010sequencing and microarray studies. Nucleic Acids Res 43: e47","journal-title":"Nucleic Acids Res"},{"key":"BFEMMM202216836_CR36","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/nature23003","volume":"547","author":"U Sahin","year":"2017","unstructured":"Sahin U, Derhovanessian E, Miller M, Kloke B\u2010P, Simon P, L\u00f6wer M, Bukur V, Tadmor AD, Luxemburger U, Schr\u00f6rs B et\u00a0al (2017) Personalized RNA mutanome vaccines mobilize poly\u2010specific therapeutic immunity against cancer. Nature 547: 222\u2013226","journal-title":"Nature"},{"key":"BFEMMM202216836_CR37","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/s41586-020-2537-9","volume":"585","author":"U Sahin","year":"2020","unstructured":"Sahin U, Oehm P, Derhovanessian E, Jabulowsky RA, Vormehr M, Gold M, Maurus D, Schwarck\u2010Kokarakis D, Kuhn AN, Omokoko T et\u00a0al (2020) An RNA vaccine drives immunity in checkpoint\u2010inhibitor\u2010treated melanoma. Nature 585: 107\u2013112","journal-title":"Nature"},{"key":"BFEMMM202216836_CR38","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/s41575-021-00438-0","volume":"18","author":"B Sangro","year":"2021","unstructured":"Sangro B, Sarobe P, Herv\u00e1s\u2010Stubbs S, Melero I (2021) Advances in immunotherapy for hepatocellular carcinoma. Nat Rev Gastroenterol Hepatol 18: 525\u2013543","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"BFEMMM202216836_CR39","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1126\/science.aaa4971","volume":"348","author":"TN Schumacher","year":"2015","unstructured":"Schumacher TN, Schreiber RD (2015) Neoantigens in cancer immunotherapy. Science 348: 69\u201374","journal-title":"Science"},{"key":"BFEMMM202216836_CR40","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/nri2858","volume":"10","author":"AW Thomson","year":"2010","unstructured":"Thomson AW, Knolle PA (2010) Antigen\u2010presenting cell function in the tolerogenic liver environment. Nat Rev Immunol 10: 753\u2013766","journal-title":"Nat Rev Immunol"},{"key":"BFEMMM202216836_CR41","doi-asserted-by":"crossref","first-page":"17727","DOI":"10.1073\/pnas.2002880117","volume":"117","author":"A Ukidve","year":"2020","unstructured":"Ukidve A, Zhao Z, Fehnel A, Krishnan V, Pan DC, Gao Y, Mandal A, Muzykantov V, Mitragotri S (2020) Erythrocyte\u2010driven immunization via biomimicry of their natural antigen\u2010presenting function. Proc Natl Acad Sci USA 117: 17727\u201317736","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMMM202216836_CR42","volume":"9","author":"Y Wang","year":"2022","unstructured":"Wang Y, Zhao Q, Zhao B, Zheng Y, Zhuang Q, Liao N, Wang P, Cai Z, Zhang D, Zeng Y et\u00a0al (2022) Remodeling tumor\u2010associated neutrophils to enhance dendritic cell\u2010based HCC neoantigen nano\u2010vaccine efficiency. Adv Sci 9: 2105631","journal-title":"Adv Sci"},{"key":"BFEMMM202216836_CR43","volume":"23","author":"M Wu","year":"2020","unstructured":"Wu M, Zheng D, Zhang D, Yu P, Peng L, Chen F, Lin Z, Cai Z, Li J, Wei Z et\u00a0al (2020) Converting immune cold into hot by biosynthetic functional vesicles to boost systematic antitumor immunity. iScience 23: 101341","journal-title":"iScience"},{"key":"BFEMMM202216836_CR44","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1038\/s41565-020-00781-4","volume":"15","author":"J Xu","year":"2020","unstructured":"Xu J, Lv J, Zhuang Q, Yang Z, Cao Z, Xu L, Pei P, Wang C, Wu H, Dong Z et\u00a0al (2020) A general strategy towards personalized nanovaccines based on fluoropolymers for post\u2010surgical cancer immunotherapy. Nat Nanotechnol 15: 1043\u20131052","journal-title":"Nat Nanotechnol"},{"key":"BFEMMM202216836_CR45","first-page":"A481","volume":"9","author":"M Yarchoan","year":"2021","unstructured":"Yarchoan M, Gane E, Marron T, Rochestie S, Cooch N, Peters J, Csiki I, Perales\u2010Puchalt A, Sardesai N (2021) 453 Personalized DNA neoantigen vaccine (GNOS\u2010PV02) in combination with plasmid IL\u201012 and pembrolizumab for the treatment of patients with advanced hepatocellular carcinoma. J Immunother Cancer 9: A481","journal-title":"J Immunother Cancer"},{"key":"BFEMMM202216836_CR46","volume":"8","author":"Y Zeng","year":"2020","unstructured":"Zeng Y, Zhang W, Li Z, Zheng Y, Wang Y, Chen G, Qiu L, Ke K, Su X, Cai Z (2020) Personalized neoantigen\u2010based immunotherapy for advanced collecting duct carcinoma: case report. J Immunother Cancer 8: e000217","journal-title":"J Immunother Cancer"},{"key":"BFEMMM202216836_CR47","volume":"7","author":"Y Zhai","year":"2021","unstructured":"Zhai Y, He X, Li Y, Han R, Ma Y, Gao P, Qian Z, Gu Y, Li S (2021) A splenic\u2010targeted versatile antigen courier: iPSC wrapped in coalescent erythrocyte\u2010liposome as tumor nanovaccine. Sci Adv 7: eabi6326","journal-title":"Sci Adv"},{"key":"BFEMMM202216836_CR48","volume":"35","author":"L Zhang","year":"2020","unstructured":"Zhang L, Sun X, Jia Y, Liu X, Dong M, Xu ZP, Liu R (2020) Nanovaccine's rapid induction of anti\u2010tumor immunity significantly improves malignant cancer immunotherapy. Nano Today 35: 100923","journal-title":"Nano Today"},{"key":"BFEMMM202216836_CR49","volume":"8","author":"D Zhang","year":"2021","unstructured":"Zhang D, Lin Z, Wu M, Cai Z, Zheng Y, He L, Li Z, Zhou J, Sun L, Chen G et\u00a0al (2021a) Cytosolic delivery of thiolated neoantigen nano\u2010vaccine combined with immune checkpoint blockade to boost anti\u2010cancer T cell immunity. Adv Sci 8: 2003504","journal-title":"Adv Sci"},{"key":"BFEMMM202216836_CR50","volume":"118","author":"H Zhang","year":"2021","unstructured":"Zhang H, You X, Wang X, Cui L, Wang Z, Xu F, Li M, Yang Z, Liu J, Huang P et\u00a0al (2021b) Delivery of mRNA vaccine with a lipid\u2010like material potentiates antitumor efficacy through Toll\u2010like receptor 4 signaling. Proc Natl Acad Sci USA 118: e2005191118","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMMM202216836_CR51","volume":"5","author":"Z Zhao","year":"2019","unstructured":"Zhao Z, Ukidve A, Gao Y, Kim J, Mitragotri S (2019) Erythrocyte leveraged chemotherapy (ELeCt): Nanoparticle assembly on erythrocyte surface to combat lung metastasis. Sci Adv 5: eaax9250","journal-title":"Sci Adv"},{"key":"BFEMMM202216836_CR52","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/s41551-020-00644-2","volume":"5","author":"Z Zhao","year":"2021","unstructured":"Zhao Z, Ukidve A, Krishnan V, Fehnel A, Pan DC, Gao Y, Kim J, Evans MA, Mandal A, Guo J et\u00a0al (2021) Systemic tumour suppression via the preferential accumulation of erythrocyte\u2010anchored chemokine\u2010encapsulating nanoparticles in lung metastases. Nat Biomed Eng 5: 441\u2013454","journal-title":"Nat Biomed Eng"},{"key":"BFEMMM202216836_CR53","volume":"34","author":"J Zhao","year":"2022","unstructured":"Zhao J, Xu Y, Ma S, Wang Y, Huang Z, Qu H, Yao H, Zhang Y, Wu G, Huang L et\u00a0al (2022a) A minimalist binary vaccine carrier for personalized postoperative cancer vaccine therapy. Adv Mater 34: 2109254","journal-title":"Adv Mater"},{"key":"BFEMMM202216836_CR54","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1021\/acs.nanolett.1c04977","volume":"22","author":"Q Zhao","year":"2022","unstructured":"Zhao Q, Wang Y, Zhao B, Chen H, Cai Z, Zheng Y, Zeng Y, Zhang D, Liu X (2022b) Neoantigen immunotherapeutic\u2010gel combined with TIM\u20103 blockade effectively restrains orthotopic hepatocellular carcinoma progression. Nano Lett 22: 2048\u20132058","journal-title":"Nano Lett"},{"key":"BFEMMM202216836_CR55","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1038\/s41467-017-02191-y","volume":"8","author":"G Zhu","year":"2017","unstructured":"Zhu G, Lynn GM, Jacobson O, Chen K, Liu Y, Zhang H, Ma Y, Zhang F, Tian R, Ni Q et\u00a0al (2017) Albumin\/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy. Nat Commun 8: 1954","journal-title":"Nat Commun"}],"container-title":["EMBO Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/emmm.202216836.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/emmm.202216836\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/emmm.202216836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/emmm.202216836.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T06:47:23Z","timestamp":1779086843000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/emmm.202216836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,8]]},"references-count":55,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10,11]]}},"alternative-id":["BFEMMM202216836"],"URL":"https:\/\/doi.org\/10.15252\/emmm.202216836","relation":{},"ISSN":["1757-4676","1757-4684"],"issn-type":[{"value":"1757-4676","type":"print"},{"value":"1757-4684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,8]]},"assertion":[{"value":"3 September 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 July 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 July 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 August 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"EMMM202216836"}}