{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:19:14Z","timestamp":1790306354522,"version":"4.1.0"},"reference-count":60,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1179906"],"award-info":[{"award-number":["OPP1179906"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS|NIH|National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["5R44AI058375"],"award-info":[{"award-number":["5R44AI058375"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS|NIH|National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["5R44AI055229"],"award-info":[{"award-number":["5R44AI055229"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Mol Med"],"published-print":{"date-parts":[[2023,6,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human monoclonal antibodies (mAbs) against the central repeat and junction domain of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    circumsporozoite protein (PfCSP) have been studied extensively to guide malaria vaccine design compared to antibodies against the PfCSP C terminus. Here, we describe the molecular characteristics and protective potential of 73 germline and mutated human mAbs against the highly immunogenic PfCSP C\u2010terminal domain. Two mAbs recognized linear epitopes in the C\u2010terminal linker with sequence similarity to repeat and junction motifs, whereas all others targeted conformational epitopes in the \u03b1\u2010thrombospondin repeat (\u03b1\u2010TSR) domain. Specificity for the polymorphic Th2R\/Th3R but not the conserved RII+\/CS.T3 region in the \u03b1\u2010TSR was associated with\n                    <jats:italic>IGHV3\u201021<\/jats:italic>\n                    \/\n                    <jats:italic>IGVL3\u201021<\/jats:italic>\n                    or\n                    <jats:italic>IGLV3\u20101<\/jats:italic>\n                    gene usage. Although the C terminus specific mAbs showed signs of more efficient affinity maturation and class\u2010switching compared to anti\u2010repeat mAbs, live sporozoite binding and inhibitory activity was limited to a single C\u2010linker reactive mAb with cross\u2010reactivity to the central repeat and junction. The data provide novel insights in the human anti\u2010C\u2010linker and anti\u2010\u03b1\u2010TSR antibody response that support exclusion of the PfCSP C terminus from malaria vaccine designs.\n                  <\/jats:p>","DOI":"10.15252\/emmm.202317454","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T03:14:27Z","timestamp":1682046867000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Molecular and functional properties of human Plasmodium falciparum CSP C\u2010terminus antibodies"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4547-0589","authenticated-orcid":false,"given":"Opeyemi Ernest","family":"Oludada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7387-6589","authenticated-orcid":false,"given":"Giulia","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8940-5423","authenticated-orcid":false,"given":"Clare","family":"Burn Aschner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna S","family":"Obraztsova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Prieto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Canetta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L","family":"Hoffman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter G","family":"Kremsner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9101-2768","authenticated-orcid":false,"given":"Benjamin","family":"Mordm\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0336-7268","authenticated-orcid":false,"given":"Rajagopal","family":"Murugan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7602-3995","authenticated-orcid":false,"given":"Jean\u2010Philippe","family":"Julien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4605-906X","authenticated-orcid":false,"given":"Elena A","family":"Levashina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3921-5933","authenticated-orcid":false,"given":"Hedda","family":"Wardemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"BFEMMM202317454_CR1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams PD, Afonine PV, Bunkoczi G, Chen VB, Davis IW, Echols N, Headd JJ, Hung LW, Kapral GJ, Grosse\u2010Kunstleve RW et\u00a0al (2010) PHENIX: a comprehensive python\u2010based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr 66: 213\u2013221","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFEMMM202317454_CR2","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1056\/NEJMoa1102287","volume":"365","author":"ST Agnandji","year":"2011","unstructured":"Agnandji ST, Lell B, Soulanoudjingar SS, Fernandes JF, Abossolo BP, Conzelmann C, Methogo BG, Doucka Y, Flamen A, Mordmuller B et\u00a0al (2011) First results of phase 3 trial of RTS,S\/AS01 malaria vaccine in African children. N Engl J Med 365: 1863\u20131875","journal-title":"N Engl J Med"},{"key":"BFEMMM202317454_CR3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0062427","volume":"8","author":"NR Aragam","year":"2013","unstructured":"Aragam NR, Thayer KM, Nge N, Hoffman I, Martinson F, Kamwendo D, Lin FC, Sutherland C, Bailey JA, Juliano JJ (2013) Diversity of T cell epitopes in Plasmodium falciparum circumsporozoite protein likely due to protein\u2010protein interactions. PLoS One 8: e62427","journal-title":"PLoS One"},{"key":"BFEMMM202317454_CR4","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/S1473-3099(11)70100-1","volume":"11","author":"KP Asante","year":"2011","unstructured":"Asante KP, Abdulla S, Agnandji S, Lyimo J, Vekemans J, Soulanoudjingar S, Owusu R, Shomari M, Leach A, Jongert E et\u00a0al (2011) Safety and efficacy of\u00a0the RTS,S\/AS01E candidate malaria vaccine given with expanded\u2010programme\u2010on\u2010immunisation vaccines: 19 month follow\u2010up of a randomised, open\u2010label, phase 2 trial. Lancet Infect Dis 11: 741\u2013749","journal-title":"Lancet Infect Dis"},{"key":"BFEMMM202317454_CR5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010409","volume":"18","author":"N Beutler","year":"2022","unstructured":"Beutler N, Pholcharee T, Oyen D, Flores\u2010Garcia Y, MacGill RS, Garcia E, Calla J, Parren M, Yang L, Volkmuth W et\u00a0al (2022) A novel CSP C\u2010terminal epitope targeted by an antibody with protective activity against Plasmodium falciparum. PLoS Pathog 18: e1010409","journal-title":"PLoS Pathog"},{"key":"BFEMMM202317454_CR6","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1016\/S0140-6736(01)06957-4","volume":"358","author":"KA Bojang","year":"2001","unstructured":"Bojang KA, Milligan PJ, Pinder M, Vigneron L, Alloueche A, Kester KE, Ballou WR, Conway DJ, Reece WH, Gothard P et\u00a0al (2001) Efficacy of RTS,S\/AS02 malaria vaccine against Plasmodium falciparum infection in semi\u2010immune adult men in The Gambia: a randomised trial. Lancet 358: 1927\u20131934","journal-title":"Lancet"},{"key":"BFEMMM202317454_CR57","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1002\/eji.201343917","volume":"44","author":"CE Busse","year":"2014","unstructured":"Busse CE, Czogiel I, Braun P, Arndt PF, Wardemann H (2014) Single-cell based high-throughput sequencing of full-length immunoglobulin heavy and light chain genes. Eur J Immunol 44: 597\u2013603","journal-title":"Eur J Immunol"},{"key":"BFEMMM202317454_CR7","doi-asserted-by":"crossref","first-page":"4880","DOI":"10.1016\/j.vaccine.2010.05.033","volume":"28","author":"S Casares","year":"2010","unstructured":"Casares S, Brumeanu TD, Richie TL (2010) The RTS,S malaria vaccine. Vaccine 28: 4880\u20134894","journal-title":"Vaccine"},{"key":"BFEMMM202317454_CR8","doi-asserted-by":"crossref","first-page":"3793","DOI":"10.1016\/j.vaccine.2019.05.059","volume":"37","author":"A Cawlfield","year":"2019","unstructured":"Cawlfield A, Genito CJ, Beck Z, Bergmann\u2010Leitner ES, Bitzer AA, Soto K, Zou X, Hadiwidjojo SH, Gerbasi RV, Mullins AB et\u00a0al (2019) Safety, toxicity and immunogenicity of a malaria vaccine based on the circumsporozoite protein (FMP013) with the adjuvant army liposome formulation containing QS21 (ALFQ). Vaccine 37: 3793\u20133803","journal-title":"Vaccine"},{"key":"BFEMMM202317454_CR9","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1186\/s12936-016-1348-9","volume":"15","author":"S Chaudhury","year":"2016","unstructured":"Chaudhury S, Ockenhouse CF, Regules JA, Dutta S, Wallqvist A, Jongert E, Waters NC, Lemiale F, Bergmann\u2010Leitner E (2016) The biological function of antibodies induced by the RTS,S\/AS01 malaria vaccine candidate is determined by their fine specificity. Malar J 15: 301","journal-title":"Malar J"},{"key":"BFEMMM202317454_CR10","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/j.vaccine.2020.12.055","volume":"39","author":"S Chaudhury","year":"2021","unstructured":"Chaudhury S, MacGill RS, Early AM, Bolton JS, King CR, Locke E, Pierson T, Wirth DF, Neafsey DE, Bergmann\u2010Leitner ES (2021) Breadth of humoral immune responses to the C\u2010terminus of the circumsporozoite protein is associated with protective efficacy induced by the RTS,S malaria vaccine. Vaccine 39: 968\u2013975","journal-title":"Vaccine"},{"key":"BFEMMM202317454_CR11","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1084\/jem.20040989","volume":"201","author":"A Coppi","year":"2005","unstructured":"Coppi A, Pinzon\u2010Ortiz C, Hutter C, Sinnis P (2005) The Plasmodium circumsporozoite protein is proteolytically processed during cell invasion. J Exp Med 201: 27\u201333","journal-title":"J Exp Med"},{"key":"BFEMMM202317454_CR12","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1126\/science.6204383","volume":"225","author":"JB Dame","year":"1984","unstructured":"Dame JB, Williams JL, McCutchan TF, Weber JL, Wirtz RA, Hockmeyer WT, Maloy WL, Haynes JD, Schneider I, Roberts D et\u00a0al (1984) Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. Science 225: 593\u2013599","journal-title":"Science"},{"key":"BFEMMM202317454_CR13","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1038\/s41467-019-10195-z","volume":"10","author":"C Dobano","year":"2019","unstructured":"Dobano C, Sanz H, Sorgho H, Dosoo D, Mpina M, Ubillos I, Aguilar R, Ford T, Diez\u2010Padrisa N, Williams NA et\u00a0al (2019) Concentration and avidity of antibodies to different circumsporozoite epitopes correlate with RTS,S\/AS01E malaria vaccine efficacy. Nat Commun 10: 2174","journal-title":"Nat Commun"},{"key":"BFEMMM202317454_CR14","doi-asserted-by":"crossref","first-page":"7817","DOI":"10.1073\/pnas.1205737109","volume":"109","author":"MB Doud","year":"2012","unstructured":"Doud MB, Koksal AC, Mi LZ, Song G, Lu C, Springer TA (2012) Unexpected fold in the circumsporozoite protein target of malaria vaccines. Proc Natl Acad Sci U S A 109: 7817\u20137822","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BFEMMM202317454_CR15","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley P, Lohkamp B, Scott WG, Cowtan K (2010) Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66: 486\u2013501","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFEMMM202317454_CR16","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/infdis\/jiv154","volume":"212","author":"DA Espinosa","year":"2015","unstructured":"Espinosa DA, Gutierrez GM, Rojas\u2010Lopez M, Noe AR, Shi L, Tse SW, Sinnis P, Zavala F (2015) Proteolytic cleavage of the Plasmodium falciparum circumsporozoite protein is a target of protective antibodies. J Infect Dis 212: 1111\u20131119","journal-title":"J Infect Dis"},{"key":"BFEMMM202317454_CR17","doi-asserted-by":"crossref","first-page":"775","DOI":"10.4269\/ajtmh.2012.11-0478","volume":"86","author":"K Gandhi","year":"2012","unstructured":"Gandhi K, Thera MA, Coulibaly D, Traore K, Guindo AB, Doumbo OK, Takala\u2010Harrison S, Plowe CV (2012) Next generation sequencing to detect variation in the Plasmodium falciparum circumsporozoite protein. Am J Trop Med Hyg 86: 775\u2013781","journal-title":"Am J Trop Med Hyg"},{"key":"BFEMMM202317454_CR18","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1016\/j.vaccine.2017.05.070","volume":"35","author":"CJ Genito","year":"2017","unstructured":"Genito CJ, Beck Z, Phares TW, Kalle F, Limbach KJ, Stefaniak ME, Patterson NB, Bergmann\u2010Leitner ES, Waters NC, Matyas GR et\u00a0al (2017) Liposomes containing monophosphoryl lipid A and QS\u201021 serve as an effective adjuvant for soluble circumsporozoite protein malaria vaccine FMP013. Vaccine 35: 3865\u20133874","journal-title":"Vaccine"},{"key":"BFEMMM202317454_CR19","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1093\/infdis\/171.6.1576","volume":"171","author":"DM Gordon","year":"1995","unstructured":"Gordon DM, McGovern TW, Krzych U, Cohen JC, Schneider I, LaChance R, Heppner DG, Yuan G, Hollingdale M, Slaoui M et\u00a0al (1995) Safety, immunogenicity, and efficacy of a recombinantly produced Plasmodium falciparum circumsporozoite protein\u2010hepatitis B surface antigen subunit vaccine. J Infect Dis 171: 1576\u20131585","journal-title":"J Infect Dis"},{"key":"BFEMMM202317454_CR20","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1093\/bioinformatics\/btv359","volume":"31","author":"NT Gupta","year":"2015","unstructured":"Gupta NT, Vander Heiden JA, Uduman M, Gadala\u2010Maria D, Yaari G, Kleinstein SH (2015) Change\u2010O: a toolkit for analyzing large\u2010scale B cell immunoglobulin repertoire sequencing data. Bioinformatics 31: 3356\u20133358","journal-title":"Bioinformatics"},{"key":"BFEMMM202317454_CR58","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001112","volume":"6","author":"C Harris","year":"2010","unstructured":"Harris C, Lambrechts L, Rousset F, Abate L, Nsango SE, Fontenille D, Morlais I, Cohuet A (2010) Polymorphisms in Anopheles gambiae immune genes associated with natural resistance to Plasmodium falciparum. PLoS Pathog 6: e1001112","journal-title":"PLoS Pathog"},{"key":"BFEMMM202317454_CR21","doi-asserted-by":"crossref","first-page":"3771","DOI":"10.1128\/IAI.02676-14","volume":"83","author":"R Herrera","year":"2015","unstructured":"Herrera R, Anderson C, Kumar K, Molina\u2010Cruz A, Nguyen V, Burkhardt M, Reiter K, Shimp R Jr, Howard RF, Srinivasan P et\u00a0al (2015) Reversible conformational change in the Plasmodium falciparum Circumsporozoite protein masks its adhesion domains. Infect Immun 83: 3771\u20133780","journal-title":"Infect Immun"},{"key":"BFEMMM202317454_CR22","doi-asserted-by":"crossref","first-page":"5781","DOI":"10.1016\/j.vaccine.2022.08.048","volume":"40","author":"JN Hutter","year":"2022","unstructured":"Hutter JN, Robben PM, Lee C, Hamer M, Moon JE, Merino K, Zhu L, Galli H, Quinn X, Brown DR et\u00a0al (2022) First\u2010in\u2010human assessment of safety and immunogenicity of low and high doses of Plasmodium falciparum malaria protein 013 (FMP013) administered intramuscularly with ALFQ adjuvant in healthy malaria\u2010naive adults. Vaccine 40: 5781\u20135790","journal-title":"Vaccine"},{"key":"BFEMMM202317454_CR59","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s12859-016-0920-1","volume":"17","author":"K Imkeller","year":"2016","unstructured":"Imkeller K, Arndt PF, Wardemann H, Busse CE (2016) sciReptor: analysis of\u00a0single-cell level immunogobulin repertoires. BMC Bioinformatics 17: 67","journal-title":"BMC Bioinformatics"},{"key":"BFEMMM202317454_CR23","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1126\/science.aar5304","volume":"360","author":"K Imkeller","year":"2018","unstructured":"Imkeller K, Scally SW, Bosch A, Marti GP, Costa G, Triller G, Murugan R, Renna V, Jumaa H, Kremsner PG et\u00a0al (2018) Antihomotypic affinity maturation improves human B cell responses against a repetitive epitope. Science 360: 1358\u20131362","journal-title":"Science"},{"key":"BFEMMM202317454_CR24","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"W Kabsch","year":"2010","unstructured":"Kabsch W (2010) XDS. Acta Crystallogr D Biol Crystallogr 66: 125\u2013132","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFEMMM202317454_CR25","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/nm.4512","volume":"24","author":"NK Kisalu","year":"2018","unstructured":"Kisalu NK, Idris AH, Weidle C, Flores\u2010Garcia Y, Flynn BJ, Sack BK, Murphy S, Schon A, Freire E, Francica JR et\u00a0al (2018) A human monoclonal antibody prevents malaria infection by targeting a new site of vulnerability on the parasite. Nat Med 24: 408\u2013416","journal-title":"Nat Med"},{"key":"BFEMMM202317454_CR60","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/s41541-023-00653-7","volume":"8","author":"J Ludwig","year":"2023","unstructured":"Ludwig J, Scally SW, Costa G, Hoffmann S, Murugan R, Lossin J, Prieto K, Obraztcova A, Lobeto N, Franke-Fayard B et\u00a0al (2023) Glycosylated nanoparticle-based PfCSP vaccine confers long-lasting antibody responses and sterile protection in mouse malaria model. NPJ Vaccines 8: 52","journal-title":"NPJ Vaccines"},{"key":"BFEMMM202317454_CR26","doi-asserted-by":"crossref","DOI":"10.1002\/pep2.24067","volume":"110","author":"CA MacRaild","year":"2018","unstructured":"MacRaild CA, Seow J, Das SC, Norton RS (2018) Disordered epitopes as peptide vaccines. Pept Sci 110: e24067","journal-title":"Pept Sci"},{"key":"BFEMMM202317454_CR27","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"AJ McCoy","year":"2007","unstructured":"McCoy AJ, Grosse\u2010Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ (2007) Phaser crystallographic software. J Appl Crystallogr 40: 658\u2013674","journal-title":"J Appl Crystallogr"},{"key":"BFEMMM202317454_CR28","doi-asserted-by":"crossref","first-page":"11889","DOI":"10.1073\/pnas.93.21.11889","volume":"93","author":"TF McCutchan","year":"1996","unstructured":"McCutchan TF, Kissinger JC, Touray MG, Rogers MJ, Li J, Sullivan M, Braga EM, Krettli AU, Miller LH (1996) Comparison of circumsporozoite proteins from avian and mammalian malarias: biological and phylogenetic implications. Proc Natl Acad Sci U S A 93: 11889\u201311894","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BFEMMM202317454_CR29","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1084\/jem.20031550","volume":"199","author":"E Meffre","year":"2004","unstructured":"Meffre E, Schaefer A, Wardemann H, Wilson P, Davis E, Nussenzweig MC (2004) Surrogate light chain expressing human peripheral B cells produce self\u2010reactive antibodies. J Exp Med 199: 145\u2013150","journal-title":"J Exp Med"},{"key":"BFEMMM202317454_CR30","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/nature21060","volume":"542","author":"B Mordmuller","year":"2017","unstructured":"Mordmuller B, Surat G, Lagler H, Chakravarty S, Ishizuka AS, Lalremruata A, Gmeiner M, Campo JJ, Esen M, Ruben AJ et\u00a0al (2017) Sterile protection against human malaria by chemoattenuated PfSPZ vaccine. Nature 542: 445\u2013449","journal-title":"Nature"},{"key":"BFEMMM202317454_CR31","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/s41541-022-00510-z","volume":"7","author":"B Mordmuller","year":"2022","unstructured":"Mordmuller B, Sulyok Z, Sulyok M, Molnar Z, Lalremruata A, Calle CL, Bayon PG, Esen M, Gmeiner M, Held J et\u00a0al (2022) A PfSPZ vaccine immunization regimen equally protective against homologous and heterologous controlled human malaria infection. NPJ Vaccines 7: 100","journal-title":"NPJ Vaccines"},{"key":"BFEMMM202317454_CR32","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.01456","volume":"2","author":"A Morin","year":"2013","unstructured":"Morin A, Eisenbraun B, Key J, Sanschagrin PC, Timony MA, Ottaviano M, Sliz P (2013) Collaboration gets the most out of software. Elife 2: e01456","journal-title":"Elife"},{"key":"BFEMMM202317454_CR33","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.aap8029","volume":"3","author":"R Murugan","year":"2018","unstructured":"Murugan R, Buchauer L, Triller G, Kreschel C, Costa G, Pidelaserra Marti G, Imkeller K, Busse CE, Chakravarty S, Sim BKL et\u00a0al (2018) Clonal selection drives protective memory B cell responses in controlled human malaria infection. Sci Immunol 3: eaap8029","journal-title":"Sci Immunol"},{"key":"BFEMMM202317454_CR34","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1038\/s41591-020-0881-9","volume":"26","author":"R Murugan","year":"2020","unstructured":"Murugan R, Scally SW, Costa G, Mustafa G, Thai E, Decker T, Bosch A, Prieto K, Levashina EA, Julien JP et\u00a0al (2020) Evolution of protective human antibodies against Plasmodium falciparum circumsporozoite protein repeat motifs. Nat Med 26: 1135\u20131145","journal-title":"Nat Med"},{"key":"BFEMMM202317454_CR35","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1084\/jem.156.1.20","volume":"156","author":"EH Nardin","year":"1982","unstructured":"Nardin EH, Nussenzweig V, Nussenzweig RS, Collins WE, Harinasuta KT, Tapchaisri P, Chomcharn Y (1982) Circumsporozoite proteins of human malaria parasites Plasmodium falciparum and Plasmodium vivax. J Exp Med 156: 20\u201330","journal-title":"J Exp Med"},{"key":"BFEMMM202317454_CR36","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/S1473-3099(10)70262-0","volume":"11","author":"A Olotu","year":"2011","unstructured":"Olotu A, Lusingu J, Leach A, Lievens M, Vekemans J, Msham S, Lang T, Gould J, Dubois MC, Jongert E et\u00a0al (2011) Efficacy of RTS,S\/AS01E malaria vaccine and exploratory analysis on anti\u2010circumsporozoite antibody titres and protection in children aged 5\u201317 months in Kenya and Tanzania: a randomised controlled trial. Lancet Infect Dis 11: 102\u2013109","journal-title":"Lancet Infect Dis"},{"key":"BFEMMM202317454_CR37","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1056\/NEJMoa1515257","volume":"374","author":"A Olotu","year":"2016","unstructured":"Olotu A, Fegan G, Wambua J, Nyangweso G, Leach A, Lievens M, Kaslow DC, Njuguna P, Marsh K, Bejon P (2016) Seven\u2010year efficacy of RTS,S\/AS01 malaria vaccine among young African children. N Engl J Med 374: 2519\u20132529","journal-title":"N Engl J Med"},{"key":"BFEMMM202317454_CR38","doi-asserted-by":"crossref","first-page":"E10438","DOI":"10.1073\/pnas.1715812114","volume":"114","author":"D Oyen","year":"2017","unstructured":"Oyen D, Torres JL, Wille\u2010Reece U, Ockenhouse CF, Emerling D, Glanville J, Volkmuth W, Flores\u2010Garcia Y, Zavala F, Ward AB et\u00a0al (2017) Structural basis for antibody recognition of the NANP repeats in Plasmodium falciparum circumsporozoite protein. Proc Natl Acad Sci U S A 114: E10438\u2013E10445","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BFEMMM202317454_CR39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1002255","volume":"13","author":"J Pompon","year":"2015","unstructured":"Pompon J, Levashina EA (2015) A new role of the mosquito complement\u2010like Cascade in male fertility in Anopheles gambiae. PLoS Biol 13: e1002255","journal-title":"PLoS Biol"},{"key":"BFEMMM202317454_CR40","doi-asserted-by":"crossref","first-page":"708","DOI":"10.4269\/ajtmh.2006.74.708","volume":"74","author":"J Sattabongkot","year":"2006","unstructured":"Sattabongkot J, Yimamnuaychoke N, Leelaudomlipi S, Rasameesoraj M, Jenwithisuk R, Coleman RE, Udomsangpetch R, Cui L, Brewer TG (2006) Establishment of a human hepatocyte line that supports in vitro development of the exo\u2010erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax. Am J Trop Med Hyg 74: 708\u2013715","journal-title":"Am J Trop Med Hyg"},{"key":"BFEMMM202317454_CR41","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1084\/jem.20170869","volume":"215","author":"SW Scally","year":"2018","unstructured":"Scally SW, Murugan R, Bosch A, Triller G, Costa G, Mordmuller B, Kremsner PG, Sim BKL, Hoffman SL, Levashina EA et\u00a0al (2018) Rare PfCSP C\u2010terminal antibodies induced by live sporozoite vaccination are ineffective against malaria infection. J Exp Med 215: 63\u201375","journal-title":"J Exp Med"},{"key":"BFEMMM202317454_CR42","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1056\/NEJM199701093360202","volume":"336","author":"JA Stoute","year":"1997","unstructured":"Stoute JA, Slaoui M, Heppner DG, Momin P, Kester KE, Desmons P, Wellde BT, Garcon N, Krzych U, Marchand M (1997) A preliminary evaluation of a recombinant circumsporozoite protein vaccine against Plasmodium falciparum malaria. RTS,S Malaria Vaccine Evaluation Group. N Engl J Med 336: 86\u201391","journal-title":"N Engl J Med"},{"key":"BFEMMM202317454_CR43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005606","volume":"12","author":"KE Swearingen","year":"2016","unstructured":"Swearingen KE, Lindner SE, Shi L, Shears MJ, Harupa A, Hopp CS, Vaughan AM, Springer TA, Moritz RL, Kappe SH et\u00a0al (2016) Interrogating the Plasmodium Sporozoite surface: identification of surface\u2010exposed proteins and demonstration of glycosylation on CSP and TRAP by mass spectrometry\u2010based proteomics. PLoS Pathog 12: e1005606","journal-title":"PLoS Pathog"},{"key":"BFEMMM202317454_CR44","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/nm.4513","volume":"24","author":"J Tan","year":"2018","unstructured":"Tan J, Sack BK, Oyen D, Zenklusen I, Piccoli L, Barbieri S, Foglierini M, Fregni CS, Marcandalli J, Jongo S et\u00a0al (2018) A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein. Nat Med 24: 401\u2013407","journal-title":"Nat Med"},{"key":"BFEMMM202317454_CR45","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20200061","volume":"217","author":"E Thai","year":"2020","unstructured":"Thai E, Costa G, Weyrich A, Murugan R, Oyen D, Flores\u2010Garcia Y, Prieto K, Bosch A, Valleriani A, Wu NC et\u00a0al (2020) A high\u2010affinity antibody against the CSP N\u2010terminal domain lacks Plasmodium falciparum inhibitory activity. J Exp Med 217: e20200061","journal-title":"J Exp Med"},{"key":"BFEMMM202317454_CR46","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.immuni.2017.11.007","volume":"47","author":"G Triller","year":"2017","unstructured":"Triller G, Scally SW, Costa G, Pissarev M, Kreschel C, Bosch A, Marois E, Sack BK, Murugan R, Salman AM et\u00a0al (2017) Natural parasite exposure induces protective human anti\u2010malarial antibodies. Immunity 47: 1197\u20131209.e10","journal-title":"Immunity"},{"key":"BFEMMM202317454_CR47","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abm9644","volume":"7","author":"I Wahl","year":"2022","unstructured":"Wahl I, Obraztsova AS, Puchan J, Hundsdorfer R, Chakravarty S, Sim BKL, Hoffman SL, Kremsner PG, Mordmuller B, Wardemann H (2022) Clonal evolution and TCR specificity of the human TFH cell response to Plasmodium falciparum CSP. Sci Immunol 7: eabm9644","journal-title":"Sci Immunol"},{"key":"BFEMMM202317454_CR48","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1111\/j.1462-5822.2005.00579.x","volume":"7","author":"Q Wang","year":"2005","unstructured":"Wang Q, Fujioka H, Nussenzweig V (2005) Mutational analysis of the GPI\u2010anchor addition sequence from the circumsporozoite protein of Plasmodium. Cell Microbiol 7: 1616\u20131626","journal-title":"Cell Microbiol"},{"key":"BFEMMM202317454_CR49","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.immuni.2020.08.014","volume":"53","author":"LT Wang","year":"2020","unstructured":"Wang LT, Pereira LS, Flores\u2010Garcia Y, O'Connor J, Flynn BJ, Schon A, Hurlburt NK, Dillon M, Yang ASP, Fabra\u2010Garcia A et\u00a0al (2020) A potent anti\u2010malarial human monoclonal antibody targets circumsporozoite protein minor repeats and neutralizes sporozoites in the liver. Immunity 53: 733\u2013744.e8","journal-title":"Immunity"},{"key":"BFEMMM202317454_CR50","volume":"17","author":"LT Wang","year":"2021","unstructured":"Wang LT, Pereira LS, Kiyuka PK, Schon A, Kisalu NK, Vistein R, Dillon M, Bonilla BG, Molina\u2010Cruz A, Barillas\u2010Mury C et\u00a0al (2021) Protective effects\u00a0of combining monoclonal antibodies and vaccines against the Plasmodium falciparum circumsporozoite protein. PLoS Pathog 17: e1010133","journal-title":"PLoS Pathog"},{"key":"BFEMMM202317454_CR51","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-4939-9151-8_5","volume":"1956","author":"H Wardemann","year":"2019","unstructured":"Wardemann H, Busse CE (2019) Expression cloning of antibodies from single human B cells. Methods Mol Biol 1956: 105\u2013125","journal-title":"Methods Mol Biol"},{"key":"BFEMMM202317454_CR52","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1126\/science.1086907","volume":"301","author":"H Wardemann","year":"2003","unstructured":"Wardemann H, Yurasov S, Schaefer A, Young JW, Meffre E, Nussenzweig MC (2003) Predominant autoantibody production by early human B cell precursors. Science 301: 1374\u20131377","journal-title":"Science"},{"key":"BFEMMM202317454_CR53","first-page":"2021","volume-title":"World malaria report 2021","author":"WHO","year":"2021","unstructured":"WHO (2021) World malaria report 2021, p 2021. Geneva: World Health Organization"},{"key":"BFEMMM202317454_CR54","doi-asserted-by":"crossref","first-page":"358","DOI":"10.3389\/fimmu.2013.00358","volume":"4","author":"G Yaari","year":"2013","unstructured":"Yaari G, Vander Heiden JA, Uduman M, Gadala\u2010Maria D, Gupta N, Stern JN, O'Connor KC, Hafler DA, Laserson U, Vigneault F et\u00a0al (2013) Models of somatic hypermutation targeting and substitution based on synonymous mutations from high\u2010throughput immunoglobulin sequencing data. Front Immunol 4: 358","journal-title":"Front Immunol"},{"key":"BFEMMM202317454_CR55","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1084\/jem.157.6.1947","volume":"157","author":"F Zavala","year":"1983","unstructured":"Zavala F, Cochrane AH, Nardin EH, Nussenzweig RS, Nussenzweig V (1983) Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes. J Exp Med 157: 1947\u20131957","journal-title":"J Exp Med"},{"key":"BFEMMM202317454_CR56","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1126\/science.2409595","volume":"228","author":"F Zavala","year":"1985","unstructured":"Zavala F, Tam JP, Hollingdale MR, Cochrane AH, Quakyi I, Nussenzweig RS, Nussenzweig V (1985) Rationale for development of a synthetic vaccine against Plasmodium falciparum malaria. Science 228: 1436\u20131440","journal-title":"Science"}],"container-title":["EMBO Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/emmm.202317454.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.15252\/emmm.202317454\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.15252\/emmm.202317454","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.15252\/emmm.202317454.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T06:48:27Z","timestamp":1779086907000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.15252\/emmm.202317454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,21]]},"references-count":60,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,7]]}},"alternative-id":["BFEMMM202317454"],"URL":"https:\/\/doi.org\/10.15252\/emmm.202317454","relation":{},"ISSN":["1757-4676","1757-4684"],"issn-type":[{"value":"1757-4676","type":"print"},{"value":"1757-4684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,21]]},"assertion":[{"value":"19 January 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 March 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 March 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 April 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"EMMM202317454"}}