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Here, we present a double\u2010readout bioluminescence\u2010based two\u2010hybrid technology, termed LuTHy, which provides two quantitative scores in one experimental procedure when testing binary interactions. PPIs are first monitored in cells by quantification of bioluminescence resonance energy transfer (BRET) and, following cell lysis, are again quantitatively assessed by luminescence\u2010based co\u2010precipitation (LuC). The double\u2010readout procedure detects interactions with higher sensitivity than traditional single\u2010readout methods and is broadly applicable, for example, for detecting the effects of small molecules or disease\u2010causing mutations on PPIs. Applying LuTHy in a focused screen, we identified 42 interactions for the presynaptic chaperone CSP\u03b1, causative to adult\u2010onset neuronal ceroid lipofuscinosis (ANCL), a progressive neurodegenerative disease. Nearly 50% of PPIs were found to be affected when studying the effect of the disease\u2010causing missense mutations L115R and \u2206L116 in CSP\u03b1 with LuTHy. Our study presents a robust, sensitive research tool with high utility for investigating the molecular mechanisms by which disease\u2010associated mutations impair protein activity in biological systems.<\/jats:p>","DOI":"10.15252\/msb.20178071","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T05:38:32Z","timestamp":1531287512000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["LuTHy: a double\u2010readout bioluminescence\u2010based two\u2010hybrid technology for quantitative mapping of protein\u2013protein interactions in mammalian cells"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8141-6272","authenticated-orcid":false,"given":"Philipp","family":"Trepte","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Kruse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Kostova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Buntru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tempelmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Secker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-0191","authenticated-orcid":false,"given":"Lisa","family":"Diez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Schulz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konrad","family":"Klockmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Zenkner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Golusik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirstin","family":"Rau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigrid","family":"Schnoegl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig C","family":"Garner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8072-1630","authenticated-orcid":false,"given":"Erich E","family":"Wanker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,11]]},"reference":[{"key":"BFMSB178071_CR1","doi-asserted-by":"crossref","first-page":"13047","DOI":"10.1038\/ncomms13047","volume":"7","author":"A Arumughan","year":"2016","unstructured":"Arumughan A, Roske Y, Barth C, Forero LL, Bravo\u2010Rodriguez K, Redel A, Kostova S, McShane E, Opitz R, Faelber K, Rau K, Mielke T, Daumke O, Selbach M, Sanchez\u2010Garcia E, Rocks O, Pan\u00e1kov\u00e1 D, Heinemann U, Wanker EE (2016) Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers. 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