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The evolution route is particularly attractive when the organisms are either difficult to engineer or the genetic basis of the phenotype is complex. However, many desired traits, like metabolite secretion, have been inaccessible to adaptive selection due to their trade\u2010off with cell growth. Here, we utilize genome\u2010scale metabolic models to design nutrient environments for selecting lineages with enhanced metabolite secretion. To overcome the growth\u2010secretion trade\u2010off, we identify environments wherein growth becomes correlated with a secondary trait termed tacking trait. The latter is selected to be coupled with the desired trait in the application environment where the trait manifestation is required. Thus, adaptive evolution in the model\u2010designed selection environment and subsequent return to the application environment is predicted to enhance the desired trait. We experimentally validate this strategy by evolving\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    for increased secretion of aroma compounds, and confirm the predicted flux\u2010rerouting using genomic, transcriptomic, and proteomic analyses. Overall, model\u2010designed selection environments open new opportunities for predictive evolution.\n                  <\/jats:p>","DOI":"10.15252\/msb.202210980","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T12:06:09Z","timestamp":1665057969000},"page":"1-18","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Predictive evolution of metabolic phenotypes using model\u2010designed environments"],"prefix":"10.1038","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1075-7448","authenticated-orcid":false,"given":"Paula","family":"Jouhten","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Konstantinidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipa","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergej","family":"Andrejev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Grkovska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Payam","family":"Ghiachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"Beltran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9125-326X","authenticated-orcid":false,"given":"Eivind","family":"Almaas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0763-1679","authenticated-orcid":false,"given":"Albert","family":"Mas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6144-2740","authenticated-orcid":false,"given":"Jonas","family":"Warringer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0130-6111","authenticated-orcid":false,"given":"Pilar","family":"Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6166-8640","authenticated-orcid":false,"given":"Kiran R","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,6]]},"reference":[{"key":"BFMSB202210980_CR1","first-page":"1183","volume":"276","author":"AF Agrawal","year":"2009","unstructured":"Agrawal AF, Stinchcombe JR (2009) How much do genetic covariances alter the rate of adaptation? 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