{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:49:39Z","timestamp":1762112979120,"version":"build-2065373602"},"reference-count":37,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":2663,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002348","name":"Bernstein Center for Computational Neuroscience T\u00fcbingen","doi-asserted-by":"publisher","award":["01GQ0432"],"award-info":[{"award-number":["01GQ0432"]}],"id":[{"id":"10.13039\/501100002348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2006,4]]},"DOI":"10.1529\/biophysj.105.070854","type":"journal-article","created":{"date-parts":[[2005,12,30]],"date-time":"2005-12-30T20:13:28Z","timestamp":1135973608000},"page":"2534-2547","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"title":["In Situ Background Estimation in Quantitative Fluorescence Imaging"],"prefix":"10.1016","volume":"90","author":[{"given":"Tsai-Wen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei-Jung","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Brunner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Detlev","family":"Schild","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1529\/biophysj.105.070854_bib1","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1038\/nature02078","article-title":"Spontaneously emerging cortical representations of visual attributes","volume":"425","author":"Kenet","year":"2003","journal-title":"Nature"},{"key":"10.1529\/biophysj.105.070854_bib2","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1126\/science.1093173","article-title":"Synfire chains and cortical songs: temporal modules of cortical activity","volume":"304","author":"Ikegaya","year":"2004","journal-title":"Science"},{"key":"10.1529\/biophysj.105.070854_bib3","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nature03274","article-title":"Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex","volume":"433","author":"Ohki","year":"2005","journal-title":"Nature"},{"key":"10.1529\/biophysj.105.070854_bib4","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1038\/290527a0","article-title":"A nondisruptive technique for loading calcium buffers and indicators into cells","volume":"290","author":"Tsien","year":"1981","journal-title":"Nature"},{"year":"2002","series-title":"Handbook of Biological Fluorescent Probes and Research Products","author":"Haugland","key":"10.1529\/biophysj.105.070854_bib5"},{"key":"10.1529\/biophysj.105.070854_bib6","doi-asserted-by":"crossref","first-page":"7319","DOI":"10.1073\/pnas.1232232100","article-title":"In vivo two-photon calcium imaging of neuronal networks","volume":"100","author":"Stosiek","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1529\/biophysj.105.070854_bib7","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/S0896-6273(01)00518-9","article-title":"Dynamics of spontaneous activity in neocortical slices","volume":"32","author":"Mao","year":"2001","journal-title":"Neuron"},{"key":"10.1529\/biophysj.105.070854_bib8","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/74823","article-title":"Large-scale oscillatory calcium waves in the immature cortex","volume":"3","author":"Garaschuk","year":"2000","journal-title":"Nat. Neurosci."},{"key":"10.1529\/biophysj.105.070854_bib9","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/j.neuron.2005.01.047","article-title":"Functional imaging reveals rapid development of visual response properties in the zebrafish tectum","volume":"45","author":"Niell","year":"2005","journal-title":"Neuron"},{"key":"10.1529\/biophysj.105.070854_bib10","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s004240050094","article-title":"[Ca2+]i recordings from neural cells in acutely isolated cerebellar slices employing differential loading of the membrane permeant form of the calcium indicator FURA-2","volume":"431","author":"Kirischuk","year":"1996","journal-title":"Pflugers Arch. Eur. J. Physiol."},{"key":"10.1529\/biophysj.105.070854_bib11","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0006-8993(95)01209-5","article-title":"Autofluorescence as a confound in the determination of calcium levels in hippocampal slices using FURA-2\/AM dye","volume":"706","author":"Brooke","year":"1996","journal-title":"Brain Res."},{"key":"10.1529\/biophysj.105.070854_bib12","first-page":"15","article-title":"Imaging calcium concentration dynamics in small neuronal compartments","volume":"219","author":"Yasuda","year":"2004","journal-title":"Sci. STKE"},{"key":"10.1529\/biophysj.105.070854_bib13","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0143-4160(90)90059-4","article-title":"Inhibition of FURA-2 sequestration and secretion with organic anion transport blockers","volume":"11","author":"Virgilio","year":"1990","journal-title":"Cell Calcium"},{"key":"10.1529\/biophysj.105.070854_bib14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0091-679X(08)61114-0","article-title":"Practical aspects of measuring [Ca2+] with fluorescent indicators","volume":"40","author":"Kao","year":"1994","journal-title":"Methods Cell Biol."},{"key":"10.1529\/biophysj.105.070854_bib15","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J. Biol. Chem."},{"key":"10.1529\/biophysj.105.070854_bib16","doi-asserted-by":"crossref","first-page":"8171","DOI":"10.1016\/S0021-9258(18)83165-9","article-title":"Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores","volume":"264","author":"Minta","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1529\/biophysj.105.070854_bib17","doi-asserted-by":"crossref","first-page":"2655","DOI":"10.1016\/S0006-3495(00)76809-3","article-title":"Estimating intracellular calcium concentrations and buffering without wavelength ratioing","volume":"78","author":"Maravall","year":"2000","journal-title":"Biophys. J."},{"year":"2000","series-title":"Statistics and Data Analysis","author":"Tamhane","key":"10.1529\/biophysj.105.070854_bib18"},{"key":"10.1529\/biophysj.105.070854_bib19","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1109\/78.599949","article-title":"ML estimation of time and frequency offset in OFDM systems","volume":"45","author":"van de Beek","year":"1997","journal-title":"IEEE Trans. Signal Process."},{"key":"10.1529\/biophysj.105.070854_bib20","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1109\/89.496215","article-title":"A maximum-likelihood approach to stochastic matching for robust speech recognition","volume":"4","author":"Sankar","year":"1996","journal-title":"IEEE Trans. Speech Audio Process."},{"key":"10.1529\/biophysj.105.070854_bib21","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1089\/10665270050514945","article-title":"Testing for differentially expressed genes by maximum-likelihood analysis of microarray data","volume":"7","author":"Ideker","year":"2000","journal-title":"J. Comput. Biol."},{"key":"10.1529\/biophysj.105.070854_bib22","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1109\/TBME.2004.826675","article-title":"Model-based neural decoding of reaching movements: a maximum likelihood approach","volume":"51","author":"Kemere","year":"2004","journal-title":"IEEE Trans. Biomed. Eng."},{"year":"1956","series-title":"Normal Table of Xenopus laevis (Daudin)","author":"Nieuwkoop","key":"10.1529\/biophysj.105.070854_bib23"},{"key":"10.1529\/biophysj.105.070854_bib24","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1046\/j.1460-9568.2003.02448.x","article-title":"Neuronal representation of odourants in the olfactory bulb of Xenopus laevis tadpoles","volume":"17","author":"Czesnik","year":"2003","journal-title":"Eur. J. Neurosci."},{"key":"10.1529\/biophysj.105.070854_bib25","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/BF02511243","article-title":"Microcontrollers as inexpensive pulse generators and parallel processors in electrophysiological experiments","volume":"34","author":"Schild","year":"1996","journal-title":"Med. Biol. Eng. Comput."},{"key":"10.1529\/biophysj.105.070854_bib26","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1113\/jphysiol.2002.031914","article-title":"cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons","volume":"545","author":"Manzini","year":"2002","journal-title":"J. Physiol. Lond."},{"key":"10.1529\/biophysj.105.070854_bib27","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1113\/jphysiol.1995.sp020881","article-title":"Different spatial patterns of [Ca2+] increase caused by N- and L-type Ca2+ channel activation in frog olfactory bulb neurones","volume":"487","author":"Bischofberger","year":"1995","journal-title":"J. Physiol. (Lond.)"},{"key":"10.1529\/biophysj.105.070854_bib28","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1016\/S0006-3495(96)79653-4","article-title":"Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons","volume":"70","author":"Helmchen","year":"1996","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.070854_bib29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1109\/83.650848","article-title":"A pyramid approach to subpixel registration based on intensity","volume":"7","author":"Th\u00e9venaz","year":"1998","journal-title":"IEEE Trans. Image Process."},{"year":"1995","series-title":"Lessons in Estimation Theory for Signal Processing, Communications, and Control","author":"Mendel","key":"10.1529\/biophysj.105.070854_bib30"},{"year":"1994","series-title":"Communication Systems","author":"Haykin","key":"10.1529\/biophysj.105.070854_bib31"},{"key":"10.1529\/biophysj.105.070854_bib32","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0896-6273(00)00056-8","article-title":"A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons","volume":"27","author":"Kuner","year":"2000","journal-title":"Neuron"},{"key":"10.1529\/biophysj.105.070854_bib33","doi-asserted-by":"crossref","first-page":"6047","DOI":"10.1074\/jbc.275.9.6047","article-title":"Mechanism and cellular applications of a green fluorescent protein-based halide sensor","volume":"275","author":"Jayaraman","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1529\/biophysj.105.070854_bib34","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.1021\/ol051603o","article-title":"A highly potassium-selective ratiometric fluorescent indicator based on BODIPY azacrown ether excitable with visible light","volume":"7","author":"Baruah","year":"2005","journal-title":"Org. Lett."},{"key":"10.1529\/biophysj.105.070854_bib35","doi-asserted-by":"crossref","first-page":"15477","DOI":"10.1021\/bi026609p","article-title":"Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application","volume":"41","author":"Hanson","year":"2002","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.105.070854_bib36","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ceca.2005.06.026","article-title":"Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins","volume":"38","author":"Demaurex","year":"2005","journal-title":"Cell Calcium."},{"key":"10.1529\/biophysj.105.070854_bib37","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.ceca.2004.10.008","article-title":"Optical single-channel recording by imaging Ca2+ flux through individual ion channels: theoretical considerations and limits to resolution","volume":"37","author":"Shuai","year":"2005","journal-title":"Cell Calcium."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349506724353?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349506724353?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:20:10Z","timestamp":1762100410000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349506724353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4]]},"references-count":37,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["S0006349506724353"],"URL":"https:\/\/doi.org\/10.1529\/biophysj.105.070854","relation":{},"ISSN":["0006-3495"],"issn-type":[{"type":"print","value":"0006-3495"}],"subject":[],"published":{"date-parts":[[2006,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In Situ Background Estimation in Quantitative Fluorescence Imaging","name":"articletitle","label":"Article Title"},{"value":"Biophysical Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1529\/biophysj.105.070854","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 The Biophysical Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}