{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T15:42:14Z","timestamp":1774971734422,"version":"3.50.1"},"reference-count":44,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":2357,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2007,2]]},"DOI":"10.1529\/biophysj.105.078592","type":"journal-article","created":{"date-parts":[[2006,11,18]],"date-time":"2006-11-18T03:55:32Z","timestamp":1163822132000},"page":"1254-1262","source":"Crossref","is-referenced-by-count":19,"title":["Coadsorption of Human Milk Lactoferrin into the Dipalmitoylglycerolphosphatidylcholine Phospholipid Monolayer Spread at the Air\/Water Interface"],"prefix":"10.1016","volume":"92","author":[{"given":"Fausto","family":"Miano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiubo","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian R.","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeff","family":"Penfold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1529\/biophysj.105.078592_bib1","first-page":"291","article-title":"The mucocutaneous junction of the lid margin and the distribution of the tear fluid","volume":"66","author":"Wolff","year":"1946","journal-title":"Trans Ophthalm. Soc UK"},{"key":"10.1529\/biophysj.105.078592_bib2","series-title":"The Tear Film: Structure, Function and Clinical Examination","author":"Korb","year":"2002"},{"key":"10.1529\/biophysj.105.078592_bib3","first-page":"1119","article-title":"Protein levels in nonstimulated and stimulated tears of normal human subjects","volume":"31","author":"Fullard","year":"1990","journal-title":"Invest Ophth. Vis. Sci."},{"key":"10.1529\/biophysj.105.078592_bib4","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1136\/bjo.82.4.453","article-title":"Tear lipocalin and the eye\u2019s front line of defence","volume":"82","author":"Gachon","year":"1998","journal-title":"Br. J. Ophthalmol."},{"key":"10.1529\/biophysj.105.078592_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-024922-0.50005-9","article-title":"The lipid secretion of the meibomian glands","volume":"22","author":"Tiffany","year":"1987","journal-title":"Adv. Lipid Res."},{"key":"10.1529\/biophysj.105.078592_bib6","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1097\/00006324-199701000-00014","article-title":"Importance of the lipid layer in the human tear film stability and evaporation","volume":"74","author":"Craig","year":"1997","journal-title":"Optom. Vis. Sci."},{"issue":"2","key":"10.1529\/biophysj.105.078592_bib7","first-page":"533","article-title":"Tear evaporation dynamics in normal subjects and subjects with obstructive meibomian gland dysfunction","volume":"44","author":"Goto","year":"2003","journal-title":"IOVS"},{"key":"10.1529\/biophysj.105.078592_bib8","doi-asserted-by":"crossref","first-page":"S2461","DOI":"10.1088\/0953-8984\/16\/26\/019","article-title":"Effect of meibomian lipid layer on evaporation of tears","volume":"16","author":"Miano","year":"2004","journal-title":"J. Phys. Condens. Matter."},{"key":"10.1529\/biophysj.105.078592_bib9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF00919408","article-title":"Biophysical behaviour of the infant meibomian lipid layer","volume":"18","author":"Kaercher","year":"1994","journal-title":"Int. Ophthalmol."},{"key":"10.1529\/biophysj.105.078592_bib10","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1023\/A:1017987608937","article-title":"The lipid layer: the outer surface of the ocular surface tear film","volume":"21","author":"McCulley","year":"2001","journal-title":"Biosci. Rep."},{"key":"10.1529\/biophysj.105.078592_bib11","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.colsurfb.2005.05.011","article-title":"Insertion of tear proteins into a meibomian lipids film","volume":"44","author":"Miano","year":"2005","journal-title":"Colloid Surf. B"},{"key":"10.1529\/biophysj.105.078592_bib12","first-page":"3100","article-title":"Tear lipocalins: potential lipid scavenger for the corneal surface","volume":"40","author":"Glasgow","year":"1999","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1529\/biophysj.105.078592_bib13","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0006-3495(92)81582-5","article-title":"Pulmonary surfactant protein SP-C causes packing rearrangements of dipalmitoylphosphatidylcholine in spread monolayers","volume":"63","author":"Perez-Gil","year":"1992","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib14","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/S0006-3495(98)77828-2","article-title":"Differential partitioning of pulmonary surfactant protein SP-A into regions of monolayers of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylcholine\/dipalmitoylphosphatidyl glycerol","volume":"74","author":"Ruano","year":"1998","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib15","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1016\/S0006-3495(00)76504-0","article-title":"Pulmonary surfactant protein A interacts with gel-like regions in monolayers of pulmonary surfactant lipid extract","volume":"79","author":"Worthman","year":"2000","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib16","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/0021-9797(74)90355-5","article-title":"Surface chemistry of tear component analogs","volume":"49","author":"Holly","year":"1974","journal-title":"J. Coll. Interface Sci."},{"key":"10.1529\/biophysj.105.078592_bib17","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/978-1-4615-0717-8_58","article-title":"Interface properties of simplified tear-like fluids in relation to lipid and aqueous layer composition","volume":"506","author":"Miano","year":"2002","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1529\/biophysj.105.078592_bib18","unstructured":"Borchman, D., L. Huang, D. Tang, G. R. John, and M. C. Yappert. 2002. The composition, structure and protein interactions of human tear film lipid. 2002 Investigative Ophthalmology & Visual Science Meeting Abstracts. Investigative Ophthalmology & Visual Science. Supplement, 43, Abstract, 81."},{"key":"10.1529\/biophysj.105.078592_bib19","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1097\/00003226-200205000-00004","article-title":"Tolerability and safety of two new preservative-free tear film substitutes","volume":"21","author":"Dinslage","year":"2002","journal-title":"Cornea"},{"key":"10.1529\/biophysj.105.078592_bib20","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.1529\/biophysj.105.061952","article-title":"Peripheral protein adsorption to lipid-water interfaces: the free area theory","volume":"89","author":"Sugar","year":"2005","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib21","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/S0001-8686(00)00034-8","article-title":"Penetration of dissolved amphiphiles into two-dimensional aggregating lipid monolayers","volume":"86","author":"Vollhardt","year":"2000","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1529\/biophysj.105.078592_bib22","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0927-7757(01)00879-2","article-title":"Morphological investigation of mixed protein\/phospholipids monolayers","volume":"201","author":"Zhang","year":"2002","journal-title":"Coll. Surf. A: Physicochem. Eng. Asp."},{"key":"10.1529\/biophysj.105.078592_bib23","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/0167-4838(92)90346-F","article-title":"Identification of the bactericidal domain of lactoferrin","volume":"1121","author":"Bellamy","year":"1992","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1529\/biophysj.105.078592_bib24","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0002-9394(03)00203-4","article-title":"Abnormal protein profiles in tears with dry eye syndrome","volume":"136","author":"Ohashi","year":"2003","journal-title":"Am. J. Ophthalmol."},{"key":"10.1529\/biophysj.105.078592_bib25","first-page":"863","article-title":"SELDI-TOF-MS protein chip array profiling of tears from patients with dry eye","volume":"46","author":"Dalmasso","year":"2005","journal-title":"IOVS"},{"key":"10.1529\/biophysj.105.078592_bib26","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1021\/la047162j","article-title":"Surface-induced unfolding of human lactoferrin","volume":"21","author":"Lu","year":"2005","journal-title":"Langmuir"},{"key":"10.1529\/biophysj.105.078592_bib27","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1006\/jcis.1998.5680","article-title":"The denaturation of lysozyme layers adsorbed at the hydrophobic solid\/liquid interface studied by neutron reflection","volume":"206","author":"Lu","year":"1998","journal-title":"J. Colloid Interface Sci."},{"key":"10.1529\/biophysj.105.078592_bib28","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1021\/la00018a051","article-title":"Structure of mixed monolayers of dipalmitoylglycerophosphocholine and polyethylene glycol monododecyl ether at the air\/water interface determined by neutron reflection and film balance techniques","volume":"10","author":"Naumann","year":"1994","journal-title":"Langmuir"},{"key":"10.1529\/biophysj.105.078592_bib29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0927-7757(99)00567-1","article-title":"Effect of protein penetration into phospholipid monolayers: morphology and structure","volume":"171","author":"Zhao","year":"2000","journal-title":"Colloid Surf. A Physicochem. Eng. Asp."},{"key":"10.1529\/biophysj.105.078592_bib30","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0001-8686(99)00019-6","article-title":"Surfactant layers at the air\/water interface: structure and composition","volume":"84","author":"Lu","year":"2000","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1529\/biophysj.105.078592_bib31","doi-asserted-by":"crossref","first-page":"2511","DOI":"10.1016\/S0006-3495(02)75594-X","article-title":"Constant normal pressure, constant surface tension, and constant temperature molecular dynamics simulation of hydrated 1,2-dilignoceroylphosphatidylcholine monolayer","volume":"82","author":"Sun","year":"2002","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib32","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1209\/0295-5075\/4\/6\/010","article-title":"Phospholipid monolayer density distribution perpendicular to the water surface. A synchrotron x-ray reflectivity study","volume":"4","author":"Helm","year":"1987","journal-title":"Europhys. Lett."},{"key":"10.1529\/biophysj.105.078592_bib33","doi-asserted-by":"crossref","first-page":"4575","DOI":"10.1021\/bi00666a005","article-title":"Nature of thermal pretransition of synthetic phospholipids: dimyristoyl- and dipalmitoyllecithin","volume":"15","author":"Janiak","year":"1976","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.105.078592_bib34","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/0022-2836(73)90303-3","article-title":"Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases","volume":"75","author":"Tardieu","year":"1973","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.105.078592_bib35","doi-asserted-by":"crossref","first-page":"8374","DOI":"10.1021\/bi00083a042","article-title":"Contributions of hydration and steric (entropic) pressures to the interactions between phosphatidylcholine bilayers: experiments with the subgel phase","volume":"32","author":"McIntosh","year":"1993","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.105.078592_bib36","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/281499a0","article-title":"The molecular structure of lecithin dihydrate","volume":"281","author":"Pearson","year":"1979","journal-title":"Nature"},{"key":"10.1529\/biophysj.105.078592_bib37","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1016\/S0006-3495(98)77900-7","article-title":"Hydration of the dienic lipid octadecadienoylphosphatidylcholine in the lamellar phase: an infrared linear dichroism and x-ray study on headgroup orientation, water ordering, and bilayer dimensions","volume":"74","author":"Binder","year":"1998","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib38","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/S0006-3495(91)82341-4","article-title":"Orientation of specifically 13CO labelled phosphatidylcholine multilayers from polarized attenuated total reflection FT-IR spectroscopy","volume":"59","author":"Hubner","year":"1991","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib39","doi-asserted-by":"crossref","first-page":"9091","DOI":"10.1021\/la051500e","article-title":"Structure of a gel phase lipid bilayer prepared by the Langmuir-Blodgett\/Langmuir-Schaefer method characterised by sum-frequency vibrational spectroscopy","volume":"21","author":"Liu","year":"2005","journal-title":"Langmuir"},{"key":"10.1529\/biophysj.105.078592_bib40","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/BF00213578","article-title":"Conformational changes of the lecithin headgroup in monolayers at the air\/water interface","volume":"23","author":"Brumm","year":"1994","journal-title":"Eur. Biophys. J."},{"key":"10.1529\/biophysj.105.078592_bib41","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0005-2736(87)90358-0","article-title":"Conformation of packing properties of lipids: the crystal structure of sodium DMPG","volume":"896","author":"Pascher","year":"1987","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1529\/biophysj.105.078592_bib42","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/0022-2836(79)90479-0","article-title":"Neutron diffraction studies on phosphatidylcholine model membranes: I. Head group conformation","volume":"134","author":"B\u00fcldt","year":"1979","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.105.078592_bib43","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1076\/ceyr.19.1.4.5341","article-title":"Components responsible for the surface tension of human tears","volume":"19","author":"Nagyova","year":"1999","journal-title":"Curr. Eye Res."},{"key":"10.1529\/biophysj.105.078592_bib44","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.ophtha.2003.07.008","article-title":"Sequential changes of lipid tear film after the instillation of a single drop of a new emulsion eye drop in dry eye patients","volume":"111","author":"Di Pascuale","year":"2004","journal-title":"Ophthalmology"}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349507709360?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349507709360?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T10:45:22Z","timestamp":1549622722000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349507709360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["S0006349507709360"],"URL":"https:\/\/doi.org\/10.1529\/biophysj.105.078592","relation":{},"ISSN":["0006-3495"],"issn-type":[{"value":"0006-3495","type":"print"}],"subject":[],"published":{"date-parts":[[2007,2]]}}}