{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:39:07Z","timestamp":1760852347083,"version":"3.33.0"},"reference-count":47,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":1871,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biophysical Journal"],"published-print":{"date-parts":[[2008,6]]},"DOI":"10.1529\/biophysj.107.117838","type":"journal-article","created":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T01:55:07Z","timestamp":1204336507000},"page":"4454-4463","source":"Crossref","is-referenced-by-count":17,"title":["Four-\u03b1-Helix Bundle with Designed Anesthetic Binding Pockets. Part I: Structural and Dynamical Analyses"],"prefix":"10.1016","volume":"94","author":[{"given":"Dejian","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole R.","family":"Brandon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanxing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasyl","family":"Bondarenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Canlas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas S.","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1529\/biophysj.107.117838_bib1","doi-asserted-by":"crossref","first-page":"16035","DOI":"10.1073\/pnas.252522299","article-title":"Large-scale molecular dynamics simulations of general anesthetic effects on the ion channel in the fully hydrated membrane: the implication of molecular mechanisms of general anesthesia","volume":"99","author":"Tang","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1529\/biophysj.107.117838_bib2","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1529\/biophysj.106.102780","article-title":"Dynamics of firefly luciferase inhibition by general anesthetics: Gaussian and anisotropic network analyses","volume":"93","author":"Szarecka","year":"2007","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.107.117838_bib3","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1529\/biophysj.105.063396","article-title":"Anesthetic interaction with ketosteroid isomerase: insights from molecular dynamics simulations","volume":"89","author":"Yonkunas","year":"2005","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.107.117838_bib4","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0378-4274(98)00205-7","article-title":"Unifying characteristics of sites of anesthetic action revealed by combined use of anesthetics and non-anesthetics","volume":"100\u2013101","author":"Xu","year":"1998","journal-title":"Toxicol. Lett."},{"key":"10.1529\/biophysj.107.117838_bib5","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1023\/A:1020182404940","article-title":"NMR studies of drug interaction with membranes and membrane-associated proteins","volume":"22","author":"Xu","year":"2002","journal-title":"Biosci. Rep."},{"key":"10.1529\/biophysj.107.117838_bib6","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1056\/NEJMra021261","article-title":"Mechanisms of actions of inhaled anesthetics","volume":"348","author":"Campagna","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"10.1529\/biophysj.107.117838_bib7","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0031-6997(24)01337-1","article-title":"Molecular interactions between inhaled anesthetics and proteins","volume":"49","author":"Eckenhoff","year":"1997","journal-title":"Pharmacol. Rev."},{"key":"10.1529\/biophysj.107.117838_bib8","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/367607a0","article-title":"Molecular and cellular mechanisms of general anaesthesia","volume":"367","author":"Franks","year":"1994","journal-title":"Nature"},{"key":"10.1529\/biophysj.107.117838_bib9","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nrn731","article-title":"Emerging structure of the nicotinic acetylcholine receptors","volume":"3","author":"Karlin","year":"2002","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1529\/biophysj.107.117838_bib10","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/38738","article-title":"Sites of alcohol and volatile anaesthetic action on GABA(A) and glycine receptors","volume":"389","author":"Mihic","year":"1997","journal-title":"Nature"},{"key":"10.1529\/biophysj.107.117838_bib11","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.jmb.2004.12.031","article-title":"Refined structure of the nicotinic acetylcholine receptor at 4A resolution","volume":"346","author":"Unwin","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.107.117838_bib12","doi-asserted-by":"crossref","first-page":"8790","DOI":"10.1021\/bi050256n","article-title":"Structure and dynamics of the second and third transmembrane domains of human glycine receptor","volume":"44","author":"Ma","year":"2005","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.107.117838_bib13","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1021\/cr0304121","article-title":"Structure determination of membrane proteins by NMR spectroscopy","volume":"104","author":"Opella","year":"2004","journal-title":"Chem. Rev."},{"key":"10.1529\/biophysj.107.117838_bib14","doi-asserted-by":"crossref","first-page":"13058","DOI":"10.1021\/bi0350396","article-title":"NMR structure and dynamics of the second transmembrane domain of the neuronal acetylcholine receptor \u03b2 2 subunit","volume":"42","author":"Yushmanov","year":"2003","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.107.117838_bib15","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1021\/bi026767g","article-title":"NMR structure and backbone dynamics of the extended second transmembrane domain of the human neuronal glycine receptor \u03b11 subunit","volume":"42","author":"Yushmanov","year":"2003","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.107.117838_bib16","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/S0006-3495(02)75166-7","article-title":"NMR structures of the second transmembrane domain of the human glycine receptor \u03b1 (1) subunit: model of pore architecture and channel gating","volume":"83","author":"Tang","year":"2002","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.107.117838_bib17","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1002\/prot.21462","article-title":"Dynamics of heteropentameric nicotinic acetylcholine receptor: implications of the gating mechanism","volume":"68","author":"Szarecka","year":"2007","journal-title":"Proteins"},{"key":"10.1529\/biophysj.107.117838_bib18","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1021\/bi9721290","article-title":"A designed cavity in the hydrophobic core of a four-\u03b1-helix bundle improves volatile anesthetic binding affinity","volume":"37","author":"Johansson","year":"1998","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.107.117838_bib19","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/S0006-3495(00)76656-2","article-title":"A designed four-\u03b1-helix bundle that binds the volatile general anesthetic halothane with high affinity","volume":"78","author":"Johansson","year":"2000","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.107.117838_bib20","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0014-5793(00)01792-0","article-title":"Molecular dynamics simulation of four-\u03b1-helix bundles that bind the anesthetic halothane","volume":"478","author":"Davies","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1529\/biophysj.107.117838_bib21","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1021\/bm049226a","article-title":"Expression and characterization of a four-\u03b1-helix bundle protein that binds the volatile general anesthetic halothane","volume":"6","author":"Pidikiti","year":"2005","journal-title":"Biomacromolecules"},{"key":"10.1529\/biophysj.107.117838_bib22","doi-asserted-by":"crossref","unstructured":"Cui, T., V. Bondarenko, D. Ma, C. Canlas, N. R. Brandon, J. S. Johansson, Y. Xu, and P. Tang. Four-\u03b1-helix bundle with designed anesthetic binding pockets. Part II: Halothane effects on structure and dynamics. Biophys. J. 94:4464-4472.","DOI":"10.1529\/biophysj.107.117853"},{"key":"10.1529\/biophysj.107.117838_bib23","doi-asserted-by":"crossref","first-page":"5984","DOI":"10.1021\/bi00185a040","article-title":"Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation","volume":"33","author":"Farrow","year":"1994","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.107.117838_bib24","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF00197809","article-title":"NMRPipe: a multidimensional spectral processing system based on UNIX pipes","volume":"6","author":"Delaglio","year":"1995","journal-title":"J. Biomol. NMR."},{"key":"10.1529\/biophysj.107.117838_bib25","first-page":"313","article-title":"Using NMRView to visualize and analyze the NMR spectra of macromolecules","volume":"278","author":"Johnson","year":"2004","journal-title":"Methods Mol. Biol."},{"key":"10.1529\/biophysj.107.117838_bib26","unstructured":"Goddard, T. D., and D. G. Kneller. SPARKY 3 University of California, San Francisco."},{"key":"10.1529\/biophysj.107.117838_bib27","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0022-2836(03)00061-5","article-title":"Solution NMR structure of ribosome-binding factor A (RbfA), a cold-shock adaptation protein from Escherichia coli","volume":"327","author":"Huang","year":"2003","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.107.117838_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1021975923026","article-title":"MONTE: an automated Monte Carlo based approach to nuclear magnetic resonance assignment of proteins","volume":"25","author":"Hitchens","year":"2003","journal-title":"J. Biomol. NMR."},{"key":"10.1529\/biophysj.107.117838_bib29","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1023\/A:1021912002051","article-title":"Data requirements for reliable chemical shift assignments in deuterated proteins","volume":"25","author":"Hitchens","year":"2003","journal-title":"J. Biomol. NMR."},{"key":"10.1529\/biophysj.107.117838_bib30","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1006\/jmbi.1997.1284","article-title":"Torsion angle dynamics for NMR structure calculation with the new program DYANA","volume":"273","author":"Guntert","year":"1997","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.107.117838_bib31","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1023\/A:1008392405740","article-title":"Protein backbone angle restraints from searching a database for chemical shift and sequence homology","volume":"13","author":"Cornilescu","year":"1999","journal-title":"J. Biomol. NMR."},{"key":"10.1529\/biophysj.107.117838_bib32","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1023\/A:1018334207887","article-title":"Temperature dependence of 1H chemical shifts in proteins","volume":"9","author":"Baxter","year":"1997","journal-title":"J. Biomol. NMR"},{"key":"10.1529\/biophysj.107.117838_bib33","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: visual molecular dynamics","volume":"14","author":"Humphrey","year":"1996","journal-title":"J. Mol. Graph."},{"key":"10.1529\/biophysj.107.117838_bib34","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0263-7855(96)00009-4","article-title":"MOLMOL: a program for display and analysis of macromolecular structures","volume":"14","author":"Koradi","year":"1996","journal-title":"J. Mol. Graph."},{"key":"10.1529\/biophysj.107.117838_bib35","doi-asserted-by":"crossref","first-page":"2674","DOI":"10.1021\/bi9526802","article-title":"Internal mobility in the partially folded DNA binding and dimerization domains of GAL4: NMR analysis of the N-H spectral density functions","volume":"35","author":"Lefevre","year":"1996","journal-title":"Biochemistry"},{"key":"10.1529\/biophysj.107.117838_bib36","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1023\/B:JNMR.0000015404.61574.65","article-title":"Temperature-dependent spectral density analysis applied to monitoring backbone dynamics of major urinary protein-I complexed with the pheromone 2- sec-butyl-4,5-dihydrothiazole","volume":"28","author":"Krizova","year":"2004","journal-title":"J. Biomol. NMR"},{"key":"10.1529\/biophysj.107.117838_bib37","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1021\/ja017461k","article-title":"An effective method for the discrimination of motional anisotropy and chemical exchange","volume":"124","author":"Kneller","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"10.1529\/biophysj.107.117838_bib38","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1006\/jmbi.1996.0771","article-title":"The main-chain dynamics of the dynamin pleckstrin homology (PH) domain in solution: analysis of 15N relaxation with monomer\/dimer equilibration","volume":"266","author":"Fushman","year":"1997","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.107.117838_bib39","doi-asserted-by":"crossref","first-page":"4546","DOI":"10.1021\/ja00381a009","article-title":"Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity","volume":"104","author":"Lipari","year":"1982","journal-title":"J. Am. Chem. Soc."},{"key":"10.1529\/biophysj.107.117838_bib40","doi-asserted-by":"crossref","first-page":"4559","DOI":"10.1021\/ja00381a010","article-title":"Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 2. Analysis of experimental results","volume":"104","author":"Lipari","year":"1982","journal-title":"J. Am. Chem. Soc."},{"key":"10.1529\/biophysj.107.117838_bib41","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1006\/jmbi.1994.0073","article-title":"Backbone dynamics of Escherichia coli ribonuclease HI: correlations with structure and function in an active enzyme","volume":"246","author":"Mandel","year":"1995","journal-title":"J. Mol. Biol."},{"key":"10.1529\/biophysj.107.117838_bib42","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1021\/ja983961a","article-title":"A relaxation-compensated Carr-Purcell-Meiboom-Gill sequence for characterizing chemical exchange by NMR spectroscopy","volume":"121","author":"Loria","year":"1999","journal-title":"J. Am. Chem. Soc."},{"key":"10.1529\/biophysj.107.117838_bib43","doi-asserted-by":"crossref","first-page":"11341","DOI":"10.1021\/ja011300z","article-title":"Slow dynamics in folded and unfolded states of an SH3 domain","volume":"123","author":"Tollinger","year":"2001","journal-title":"J. Am. Chem. Soc."},{"key":"10.1529\/biophysj.107.117838_bib44","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/S0006-3495(99)76928-6","article-title":"Distinctly different interactions of anesthetic and nonimmobilizer with transmembrane channel peptides","volume":"77","author":"Tang","year":"1999","journal-title":"Biophys. J."},{"key":"10.1529\/biophysj.107.117838_bib45","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/S0005-2736(96)00184-8","article-title":"Amphiphilic sites for general anesthetic action? Evidence from 129Xe-{1H} intermolecular nuclear Overhauser effects","volume":"1323","author":"Xu","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1529\/biophysj.107.117838_bib46","first-page":"1098","article-title":"NORMA: a tool for flexible fitting of high-resolution protein structures into low-resolution electron-microscopy-derived density maps","volume":"62","author":"Suhre","year":"2006","journal-title":"Acta Crystallogr."},{"key":"10.1529\/biophysj.107.117838_bib47","doi-asserted-by":"crossref","first-page":"W610","DOI":"10.1093\/nar\/gkh368","article-title":"ElNemo: a normal mode web server for protein movement analysis and the generation of templates for molecular replacement","volume":"32","author":"Suhre","year":"2004","journal-title":"Nucleic Acids Res."}],"container-title":["Biophysical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349508701000?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006349508701000?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T21:17:32Z","timestamp":1738099052000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006349508701000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["S0006349508701000"],"URL":"https:\/\/doi.org\/10.1529\/biophysj.107.117838","relation":{},"ISSN":["0006-3495"],"issn-type":[{"type":"print","value":"0006-3495"}],"subject":[],"published":{"date-parts":[[2008,6]]}}}