{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T13:23:05Z","timestamp":1700745785935},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>A method is described for the quantitative estimation of C<jats:sub>19<\/jats:sub>-\u0394<jats:sup>16<\/jats:sup>-steroids in human urine. After extraction and preliminary purification by the method of <jats:italic>Brooksbank &amp; Haslewood<\/jats:italic> (1961), the steroids were analyzed as chloromethyl dimethylsilyl ethers by gas-liquid chromatography. The specificity, accuracy and precision of the method were found to be satisfactory and comparison with the colorimetric method of <jats:italic>Brooksbank &amp; Haslewood<\/jats:italic> (1961) for urinary 3\u03b1-hydroxy-5\u03b1-androst-16-ene showed a high correlation coefficient, r = 0.97 (<jats:italic>P<\/jats:italic> &lt; 0.001). Figures are given for the urinary excretion rate of 3\u03b1-hydroxy-5\u03b1-androst-16-ene of normal men and women and of some women suffering from hirsutism and from adrenocortical overactivity. The values are given for 3\u03b1-hydroxy-5\u03b2-androst-16-ene and 3\u03b2-hydroxyandrosta-5,16-diene in pools of urine from healthy men and women.<\/jats:p>","DOI":"10.1530\/acta.0.0630079","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T20:59:13Z","timestamp":1229374753000},"page":"79-90","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":12,"title":["THE ESTIMATION OF 3\u03b1-HYDROXY-5\u03b1-ANDROST-16-ENE AND OTHER C19-\u039416-STEROIDS IN URINE BY GAS-LIQUID CHROMATOGRAPHY"],"prefix":"10.1093","volume":"63","author":[{"given":"B. W. L.","family":"Brooksbank","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. B.","family":"Gower","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/63\/1\/acta_63_1_009.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/63\/1\/acta_63_1_009.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:10:27Z","timestamp":1676391027000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/63\/1\/79\/6894836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0630079","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,1]]}}}