{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:26:27Z","timestamp":1756995987218},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>A method for rapid screening of ACTH antibodies has been developed. It consists of a simplified study of the modification of the antigen-antibody equilibrium by adding standard ACTH.<\/jats:p>\n        <jats:p>Different immunization methods have been studied according to the type of antigen and its preparation, the rhythm of injections as well as the amount of ACTH injected.<\/jats:p>\n        <jats:p>Among these different methods, using 267 guinea pigs, only the injection of 25 guinea pigs with 30 IU of human ACTH adsorbed on 5 mg of vegetal charcoal made it possible to obtain 7 antibodies suitable for a specific and sensitive radioimmunoassay of ACTH in human plasma without previous extraction.<\/jats:p>\n        <jats:p>These antibodies react with the portion 17\u201324 of the ACTH molecule, and their equilibrium constants are distributed from 2.10<jats:sup>11<\/jats:sup> <jats:sc>m<\/jats:sc><jats:sup>\u22121<\/jats:sup> to 1.10<jats:sup>12<\/jats:sup> <jats:sc>m<\/jats:sc><jats:sup>\u22121<\/jats:sup>. Assays performed with one of these antibodies in the plasma of normal subjects and of patients suffering from adrenal-pituitary axis disorders are essentially identical with the values obtained by bioassay and are in good agreement with the results of radioimmunoassay performed by other investigators.<\/jats:p>","DOI":"10.1530\/acta.0.0750461","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:19:36Z","timestamp":1229375976000},"page":"461-477","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":11,"title":["PREPARATION AND EVALUATION OF ACTH ANTIBODIES"],"prefix":"10.1093","volume":"75","author":[{"given":"M. F.","family":"Proeschel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Courvalin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Donnadieu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Binoux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Girard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/75\/3\/acta_75_3_006.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/75\/3\/acta_75_3_006.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:22:06Z","timestamp":1676391726000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/75\/3\/461\/6837936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0750461","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,3]]}}}