{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T05:24:18Z","timestamp":1676438658106},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>A method of haemagglutination inhibition assay of HCS was developed utilizing formaldehyde and glutaraldehyde to conjugate the hormone to sheep red blood cells. This technique is sufficiently sensitive (8.7 ng\/ml) so that assay of HCS from early pregnancy serum (17 weeks of gestation) can be performed directly after 1:50 dilution. Results can be obtained within 3 h. HCS sensitized red blood cells were shown to be stable for several months.<\/jats:p>\n        <jats:p>HCS levels of 20 normal pregnancies were estimated throughout gestation. Forty-two sera from pregnant subjects were assayed by this technique and radioimmunoassay (RIA). The values obtained by the 2 methods had excellent correlation (r = 0.95). Reproducibility of the present method was \u00b1 15 %. Data of HCS serum levels obtained by the present technique and by RIA in high-risk pregnancies are compared and the conclusion is reached that any of these techniques can be used for the successful clinical assay of HCS.<\/jats:p>","DOI":"10.1530\/acta.0.0750584","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:19:36Z","timestamp":1229375976000},"page":"584-594","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":0,"title":["RAPID ESTIMATION OF HUMAN CHORIONIC SOMATOMAMMOTROPHIN (HCS) BY HAEMAGGLUTINATION INHIBITION AND COMPARISON OF RESULTS WITH RADIOIMMUNOASSAY"],"prefix":"10.1093","volume":"75","author":[{"given":"J.","family":"Spona","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"T\u00f6pert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/75\/3\/acta_75_3_019.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/75\/3\/acta_75_3_019.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:22:11Z","timestamp":1676391731000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/75\/3\/584\/6837913"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0750584","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,3]]}}}